Suprabasin-derived bioactive peptides identified by plasma peptidomics
Abstract Identification of low-abundance, low-molecular-weight native peptides using non-tryptic plasma has long remained an unmet challenge, leaving potential bioactive/biomarker peptides undiscovered. We have succeeded in efficiently removing high-abundance plasma proteins to enrich and comprehens...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-020-79353-4 |
| _version_ | 1818734053975654400 |
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| author | Tomomi Taguchi Yoshio Kodera Kazuhito Oba Tatsuya Saito Yuzuru Nakagawa Yusuke Kawashima Masayoshi Shichiri |
| author_facet | Tomomi Taguchi Yoshio Kodera Kazuhito Oba Tatsuya Saito Yuzuru Nakagawa Yusuke Kawashima Masayoshi Shichiri |
| author_sort | Tomomi Taguchi |
| collection | DOAJ |
| description | Abstract Identification of low-abundance, low-molecular-weight native peptides using non-tryptic plasma has long remained an unmet challenge, leaving potential bioactive/biomarker peptides undiscovered. We have succeeded in efficiently removing high-abundance plasma proteins to enrich and comprehensively identify low-molecular-weight native peptides using mass spectrometry. Native peptide sequences were chemically synthesized and subsequent functional analyses resulted in the discovery of three novel bioactive polypeptides derived from an epidermal differentiation marker protein, suprabasin. SBSN_HUMAN[279–295] potently suppressed food/water intake and induced locomotor activity when injected intraperitoneally, while SBSN_HUMAN[225–237] and SBSN_HUMAN[243–259] stimulated the expression of proinflammatory cytokines via activation of NF-κB signaling in vascular cells. SBSN_HUMAN[225–237] and SBSN_HUMAN[279–295] immunoreactivities were present in almost all human organs analyzed, while immunoreactive SBSN_HUMAN[243–259] was abundant in the liver and pancreas. Human macrophages expressed the three suprabasin-derived peptides. This study illustrates a new approach for discovering unknown bioactive peptides in plasma via the generation of peptide libraries using a novel peptidomic strategy. |
| first_indexed | 2024-12-17T23:59:15Z |
| format | Article |
| id | doaj.art-cdc5cf7e975746e1bf9c6d7737b91281 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-17T23:59:15Z |
| publishDate | 2021-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-cdc5cf7e975746e1bf9c6d7737b912812022-12-21T21:27:59ZengNature PortfolioScientific Reports2045-23222021-01-0111111710.1038/s41598-020-79353-4Suprabasin-derived bioactive peptides identified by plasma peptidomicsTomomi Taguchi0Yoshio Kodera1Kazuhito Oba2Tatsuya Saito3Yuzuru Nakagawa4Yusuke Kawashima5Masayoshi Shichiri6Department of Endocrinology, Diabetes and Metabolism, Kitasato University School of MedicineDepartment of Physics, Kitasato University School of ScienceDepartment of Endocrinology, Diabetes and Metabolism, Kitasato University School of MedicineDepartment of Endocrinology, Diabetes and Metabolism, Kitasato University School of MedicineDepartment of Physics, Kitasato University School of ScienceDepartment of Physics, Kitasato University School of ScienceDepartment of Endocrinology, Diabetes and Metabolism, Kitasato University School of MedicineAbstract Identification of low-abundance, low-molecular-weight native peptides using non-tryptic plasma has long remained an unmet challenge, leaving potential bioactive/biomarker peptides undiscovered. We have succeeded in efficiently removing high-abundance plasma proteins to enrich and comprehensively identify low-molecular-weight native peptides using mass spectrometry. Native peptide sequences were chemically synthesized and subsequent functional analyses resulted in the discovery of three novel bioactive polypeptides derived from an epidermal differentiation marker protein, suprabasin. SBSN_HUMAN[279–295] potently suppressed food/water intake and induced locomotor activity when injected intraperitoneally, while SBSN_HUMAN[225–237] and SBSN_HUMAN[243–259] stimulated the expression of proinflammatory cytokines via activation of NF-κB signaling in vascular cells. SBSN_HUMAN[225–237] and SBSN_HUMAN[279–295] immunoreactivities were present in almost all human organs analyzed, while immunoreactive SBSN_HUMAN[243–259] was abundant in the liver and pancreas. Human macrophages expressed the three suprabasin-derived peptides. This study illustrates a new approach for discovering unknown bioactive peptides in plasma via the generation of peptide libraries using a novel peptidomic strategy.https://doi.org/10.1038/s41598-020-79353-4 |
| spellingShingle | Tomomi Taguchi Yoshio Kodera Kazuhito Oba Tatsuya Saito Yuzuru Nakagawa Yusuke Kawashima Masayoshi Shichiri Suprabasin-derived bioactive peptides identified by plasma peptidomics Scientific Reports |
| title | Suprabasin-derived bioactive peptides identified by plasma peptidomics |
| title_full | Suprabasin-derived bioactive peptides identified by plasma peptidomics |
| title_fullStr | Suprabasin-derived bioactive peptides identified by plasma peptidomics |
| title_full_unstemmed | Suprabasin-derived bioactive peptides identified by plasma peptidomics |
| title_short | Suprabasin-derived bioactive peptides identified by plasma peptidomics |
| title_sort | suprabasin derived bioactive peptides identified by plasma peptidomics |
| url | https://doi.org/10.1038/s41598-020-79353-4 |
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