| Summary: | As a chemical platform, 2,3-Butanediol (2,3-BD) has been widely applied in various industrial fields. In this study, to enhance the production of 2,3-BD by <i>Saccharomyces cerevisiae</i> L7, Plackett–Burman (PB) multifactorial design, the steepest climb test and central composite design (CCD) were employed to optimize the culturing conditions of <i>S. cerevisiae</i> L7. The results show that acetic acid, peptone and glucose were contributing factors for 2,3-BD production. Subsequently, a satisfactory production of 2,3-BD (13.52 ± 0.12 g/L) was reached under optimal conditions, which was 3.12 times higher than before optimization. Furthermore, fed-batch fermentation was carried out under optimized culture conditions, and a higher production and yield efficiency of 2,3-BD were achieved (21.83 ± 0.56 g/L and 0.15 ± 0.01 g/g, respectively) when glucose (20 g/L) and acetic acid (0.2 g/L) were added at 12, 24, 36, 48 and 60 h. Therefore, the production and yield efficiency of 2,3-BD were higher than those without fed-batch fermentation (61.46% and 58.51%, respectively). These results provide good support and a technical foundation for the large-scale industrial production of 2,3-BD by <i>Saccharomyces cerevisiae</i>.
|
|---|