Production of 2,3-Butanediol by <i>S. cerevisiae</i> L7 in Fed-Batch Fermentation with Optimized Culture Conditions
As a chemical platform, 2,3-Butanediol (2,3-BD) has been widely applied in various industrial fields. In this study, to enhance the production of 2,3-BD by <i>Saccharomyces cerevisiae</i> L7, Plackett–Burman (PB) multifactorial design, the steepest climb test and central composite design...
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| Format: | Article |
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MDPI AG
2023-07-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/9/7/694 |
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| author | Guoxu Ao Shanshan Sun Lei Liu Yuhao Guo Xiujun Tu Jingping Ge Wenxiang Ping |
| author_facet | Guoxu Ao Shanshan Sun Lei Liu Yuhao Guo Xiujun Tu Jingping Ge Wenxiang Ping |
| author_sort | Guoxu Ao |
| collection | DOAJ |
| description | As a chemical platform, 2,3-Butanediol (2,3-BD) has been widely applied in various industrial fields. In this study, to enhance the production of 2,3-BD by <i>Saccharomyces cerevisiae</i> L7, Plackett–Burman (PB) multifactorial design, the steepest climb test and central composite design (CCD) were employed to optimize the culturing conditions of <i>S. cerevisiae</i> L7. The results show that acetic acid, peptone and glucose were contributing factors for 2,3-BD production. Subsequently, a satisfactory production of 2,3-BD (13.52 ± 0.12 g/L) was reached under optimal conditions, which was 3.12 times higher than before optimization. Furthermore, fed-batch fermentation was carried out under optimized culture conditions, and a higher production and yield efficiency of 2,3-BD were achieved (21.83 ± 0.56 g/L and 0.15 ± 0.01 g/g, respectively) when glucose (20 g/L) and acetic acid (0.2 g/L) were added at 12, 24, 36, 48 and 60 h. Therefore, the production and yield efficiency of 2,3-BD were higher than those without fed-batch fermentation (61.46% and 58.51%, respectively). These results provide good support and a technical foundation for the large-scale industrial production of 2,3-BD by <i>Saccharomyces cerevisiae</i>. |
| first_indexed | 2024-03-11T01:06:04Z |
| format | Article |
| id | doaj.art-cdc747331fe04021a5b28fbd15b26dfe |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-11T01:06:04Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-cdc747331fe04021a5b28fbd15b26dfe2023-11-18T19:17:07ZengMDPI AGFermentation2311-56372023-07-019769410.3390/fermentation9070694Production of 2,3-Butanediol by <i>S. cerevisiae</i> L7 in Fed-Batch Fermentation with Optimized Culture ConditionsGuoxu Ao0Shanshan Sun1Lei Liu2Yuhao Guo3Xiujun Tu4Jingping Ge5Wenxiang Ping6Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education & Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold Region & Key Laboratory of Molecular Biology, College of Heilongjiang Province & School of Life Sciences, Heilongjiang University, Harbin 150080, ChinaEngineering Research Center of Agricultural Microbiology Technology, Ministry of Education & Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold Region & Key Laboratory of Molecular Biology, College of Heilongjiang Province & School of Life Sciences, Heilongjiang University, Harbin 150080, ChinaEngineering Research Center of Agricultural Microbiology Technology, Ministry of Education & Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold Region & Key Laboratory of Molecular Biology, College of Heilongjiang Province & School of Life Sciences, Heilongjiang University, Harbin 150080, ChinaEngineering Research Center of Agricultural Microbiology Technology, Ministry of Education & Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold Region & Key Laboratory of Molecular Biology, College of Heilongjiang Province & School of Life Sciences, Heilongjiang University, Harbin 150080, ChinaEngineering Research Center of Agricultural Microbiology Technology, Ministry of Education & Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold Region & Key Laboratory of Molecular Biology, College of Heilongjiang Province & School of Life Sciences, Heilongjiang University, Harbin 150080, ChinaEngineering Research Center of Agricultural Microbiology Technology, Ministry of Education & Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold Region & Key Laboratory of Molecular Biology, College of Heilongjiang Province & School of Life Sciences, Heilongjiang University, Harbin 150080, ChinaEngineering Research Center of Agricultural Microbiology Technology, Ministry of Education & Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold Region & Key Laboratory of Molecular Biology, College of Heilongjiang Province & School of Life Sciences, Heilongjiang University, Harbin 150080, ChinaAs a chemical platform, 2,3-Butanediol (2,3-BD) has been widely applied in various industrial fields. In this study, to enhance the production of 2,3-BD by <i>Saccharomyces cerevisiae</i> L7, Plackett–Burman (PB) multifactorial design, the steepest climb test and central composite design (CCD) were employed to optimize the culturing conditions of <i>S. cerevisiae</i> L7. The results show that acetic acid, peptone and glucose were contributing factors for 2,3-BD production. Subsequently, a satisfactory production of 2,3-BD (13.52 ± 0.12 g/L) was reached under optimal conditions, which was 3.12 times higher than before optimization. Furthermore, fed-batch fermentation was carried out under optimized culture conditions, and a higher production and yield efficiency of 2,3-BD were achieved (21.83 ± 0.56 g/L and 0.15 ± 0.01 g/g, respectively) when glucose (20 g/L) and acetic acid (0.2 g/L) were added at 12, 24, 36, 48 and 60 h. Therefore, the production and yield efficiency of 2,3-BD were higher than those without fed-batch fermentation (61.46% and 58.51%, respectively). These results provide good support and a technical foundation for the large-scale industrial production of 2,3-BD by <i>Saccharomyces cerevisiae</i>.https://www.mdpi.com/2311-5637/9/7/6942,3-BD<i>Saccharomyces cerevisiae</i>Plackett–Burman designcentral composite designfed-batch fermentation |
| spellingShingle | Guoxu Ao Shanshan Sun Lei Liu Yuhao Guo Xiujun Tu Jingping Ge Wenxiang Ping Production of 2,3-Butanediol by <i>S. cerevisiae</i> L7 in Fed-Batch Fermentation with Optimized Culture Conditions Fermentation 2,3-BD <i>Saccharomyces cerevisiae</i> Plackett–Burman design central composite design fed-batch fermentation |
| title | Production of 2,3-Butanediol by <i>S. cerevisiae</i> L7 in Fed-Batch Fermentation with Optimized Culture Conditions |
| title_full | Production of 2,3-Butanediol by <i>S. cerevisiae</i> L7 in Fed-Batch Fermentation with Optimized Culture Conditions |
| title_fullStr | Production of 2,3-Butanediol by <i>S. cerevisiae</i> L7 in Fed-Batch Fermentation with Optimized Culture Conditions |
| title_full_unstemmed | Production of 2,3-Butanediol by <i>S. cerevisiae</i> L7 in Fed-Batch Fermentation with Optimized Culture Conditions |
| title_short | Production of 2,3-Butanediol by <i>S. cerevisiae</i> L7 in Fed-Batch Fermentation with Optimized Culture Conditions |
| title_sort | production of 2 3 butanediol by i s cerevisiae i l7 in fed batch fermentation with optimized culture conditions |
| topic | 2,3-BD <i>Saccharomyces cerevisiae</i> Plackett–Burman design central composite design fed-batch fermentation |
| url | https://www.mdpi.com/2311-5637/9/7/694 |
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