CeRNA Expression Profiling Identifies KIT-Related circRNA-miRNA-mRNA Networks in Gastrointestinal Stromal Tumour
Gastrointestinal stromal tumours (GISTs) are the most common human sarcomas and are typically located in the stomach or small intestine. Although circular RNAs (circRNAs) reportedly play vital roles in tumour oncogenesis and progression, the molecular basis of the aggressive tumour biology of these...
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| Format: | Article |
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Frontiers Media S.A.
2019-09-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fgene.2019.00825/full |
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| author | Ning Jia Ning Jia Hanxing Tong Yong Zhang Hiroshi Katayama Yuan Wang Weiqi Lu Sumei Zhang Jin Wang |
| author_facet | Ning Jia Ning Jia Hanxing Tong Yong Zhang Hiroshi Katayama Yuan Wang Weiqi Lu Sumei Zhang Jin Wang |
| author_sort | Ning Jia |
| collection | DOAJ |
| description | Gastrointestinal stromal tumours (GISTs) are the most common human sarcomas and are typically located in the stomach or small intestine. Although circular RNAs (circRNAs) reportedly play vital roles in tumour oncogenesis and progression, the molecular basis of the aggressive tumour biology of these circRNAs in GISTs remains unclear. In this study, we applied SBC ceRNA microarrays to screen for tumour-specific circRNA profiles in GISTs and identified that a total of 5,770 circRNAs and 1,815 mRNAs were differentially expressed in GISTs. Three significantly differential circRNAs (circ_0069765, circ_0084097, and circ_0079471) and their host genes (KIT, PLAT, and ETV1) were also verified in 68 pairs of GISTs and adjacent normal gastrointestinal tissues by qRT-PCR. A GIST-specific circRNA-miRNA-mRNA regulatory network analysis demonstrated that the specific KIT-related regulatory networks involved the three circRNAs, the circRNA host genes and three miRNAs (miR-142-5p, miR-144-3p and miR-485-3p), which may be key regulators of GISTs that could serve as molecular biomarkers and potential therapeutic targets for this malignant disease. |
| first_indexed | 2024-04-14T02:42:48Z |
| format | Article |
| id | doaj.art-cdce6c2f3a6b4361a32c56f312c07edb |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-04-14T02:42:48Z |
| publishDate | 2019-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-cdce6c2f3a6b4361a32c56f312c07edb2022-12-22T02:16:47ZengFrontiers Media S.A.Frontiers in Genetics1664-80212019-09-011010.3389/fgene.2019.00825442660CeRNA Expression Profiling Identifies KIT-Related circRNA-miRNA-mRNA Networks in Gastrointestinal Stromal TumourNing Jia0Ning Jia1Hanxing Tong2Yong Zhang3Hiroshi Katayama4Yuan Wang5Weiqi Lu6Sumei Zhang7Jin Wang8Laboratory of Molecular Biology and Department of Biochemistry, Anhui Medical University, Hefei, ChinaShanghai Public Health Clinical Center, Fudan University, Shanghai, ChinaDepartment of General Surgery, Zhongshan Hospital, Fudan University, Shanghai, ChinaDepartment of General Surgery, Zhongshan Hospital, Fudan University, Shanghai, ChinaDepartment of Molecular Oncology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, JapanLaboratory of Molecular Biology and Department of Biochemistry, Anhui Medical University, Hefei, ChinaDepartment of General Surgery, Zhongshan Hospital, Fudan University, Shanghai, ChinaLaboratory of Molecular Biology and Department of Biochemistry, Anhui Medical University, Hefei, ChinaShanghai Public Health Clinical Center, Fudan University, Shanghai, ChinaGastrointestinal stromal tumours (GISTs) are the most common human sarcomas and are typically located in the stomach or small intestine. Although circular RNAs (circRNAs) reportedly play vital roles in tumour oncogenesis and progression, the molecular basis of the aggressive tumour biology of these circRNAs in GISTs remains unclear. In this study, we applied SBC ceRNA microarrays to screen for tumour-specific circRNA profiles in GISTs and identified that a total of 5,770 circRNAs and 1,815 mRNAs were differentially expressed in GISTs. Three significantly differential circRNAs (circ_0069765, circ_0084097, and circ_0079471) and their host genes (KIT, PLAT, and ETV1) were also verified in 68 pairs of GISTs and adjacent normal gastrointestinal tissues by qRT-PCR. A GIST-specific circRNA-miRNA-mRNA regulatory network analysis demonstrated that the specific KIT-related regulatory networks involved the three circRNAs, the circRNA host genes and three miRNAs (miR-142-5p, miR-144-3p and miR-485-3p), which may be key regulators of GISTs that could serve as molecular biomarkers and potential therapeutic targets for this malignant disease.https://www.frontiersin.org/article/10.3389/fgene.2019.00825/fullcircRNAsKITPLATETV1regulatory networks analysis |
| spellingShingle | Ning Jia Ning Jia Hanxing Tong Yong Zhang Hiroshi Katayama Yuan Wang Weiqi Lu Sumei Zhang Jin Wang CeRNA Expression Profiling Identifies KIT-Related circRNA-miRNA-mRNA Networks in Gastrointestinal Stromal Tumour Frontiers in Genetics circRNAs KIT PLAT ETV1 regulatory networks analysis |
| title | CeRNA Expression Profiling Identifies KIT-Related circRNA-miRNA-mRNA Networks in Gastrointestinal Stromal Tumour |
| title_full | CeRNA Expression Profiling Identifies KIT-Related circRNA-miRNA-mRNA Networks in Gastrointestinal Stromal Tumour |
| title_fullStr | CeRNA Expression Profiling Identifies KIT-Related circRNA-miRNA-mRNA Networks in Gastrointestinal Stromal Tumour |
| title_full_unstemmed | CeRNA Expression Profiling Identifies KIT-Related circRNA-miRNA-mRNA Networks in Gastrointestinal Stromal Tumour |
| title_short | CeRNA Expression Profiling Identifies KIT-Related circRNA-miRNA-mRNA Networks in Gastrointestinal Stromal Tumour |
| title_sort | cerna expression profiling identifies kit related circrna mirna mrna networks in gastrointestinal stromal tumour |
| topic | circRNAs KIT PLAT ETV1 regulatory networks analysis |
| url | https://www.frontiersin.org/article/10.3389/fgene.2019.00825/full |
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