Chemical Composition, Antioxidant and Cytotoxicity Activities of Leaves, Bark, Twigs and Oleo-Resin of <i>Dipterocarpus alatus</i>
<i>Dipterocarpus alatus</i> (Dipterocarpaceae) is a medicinal plant whose use is well known for the treatment of genito-urinary diseases. However, there is no report of its cytotoxic potential. In this study, the chemical composition, antioxidant and cytotoxic activities of extracts of t...
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MDPI AG
2019-08-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/24/17/3083 |
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| author | Chawalit Yongram Bunleu Sungthong Ploenthip Puthongking Natthida Weerapreeyakul |
| author_facet | Chawalit Yongram Bunleu Sungthong Ploenthip Puthongking Natthida Weerapreeyakul |
| author_sort | Chawalit Yongram |
| collection | DOAJ |
| description | <i>Dipterocarpus alatus</i> (Dipterocarpaceae) is a medicinal plant whose use is well known for the treatment of genito-urinary diseases. However, there is no report of its cytotoxic potential. In this study, the chemical composition, antioxidant and cytotoxic activities of extracts of the leaves, bark, twigs and oleo-resin from <i>D. alatus</i> are investigated. Cytotoxicity was measured by the neutral red (NR) assay against HCT116, SKLU1, SK-MEL2, SiHa and U937 cancer cell lines and antioxidant capacity was evaluated by DPPH, ABTS radical scavenging, and ferric reducing antioxidant power (FRAP) assays. The chemical composition was analyzed by gas chromatography−mass spectrometry (GC-MS). Leaf, bark and twig extracts exhibited stronger antioxidant activity than oleo-resin, with bark extract showing the highest antioxidant activity and the highest total phenolic content. All samples showed more cytotoxic activity against the U937 cell line than HCT116, SKLU1, SK-MEL2 and SiHa cells with oleo-resin being more cytotoxic than melphalan against U937 cells. Chemical composition analysis of oleo-resin by GC-MS showed that the major components were sesquiterpenes, namely α-gurjunene (30.31%), (-)-isoledene (13.69%), alloaromadendrene (3.28%), β-caryophyllene (3.14%), γ-gurjunene (3.14%) and spathulenol (1.11%). The cytotoxic activity of oleo-resin can be attributed to the sesquiterpene content, whereas the cytotoxic and antioxidant activities of leaf, bark and twig extracts correlated to total phenolic content. |
| first_indexed | 2024-12-21T03:03:25Z |
| format | Article |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-21T03:03:25Z |
| publishDate | 2019-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-cdd068839d8345daa99428f8642ba6852022-12-21T19:18:08ZengMDPI AGMolecules1420-30492019-08-012417308310.3390/molecules24173083molecules24173083Chemical Composition, Antioxidant and Cytotoxicity Activities of Leaves, Bark, Twigs and Oleo-Resin of <i>Dipterocarpus alatus</i>Chawalit Yongram0Bunleu Sungthong1Ploenthip Puthongking2Natthida Weerapreeyakul3Biomedical Science Program, Graduate School, Khon Kaen University, Khon Kaen 40002, ThailandPharmaceutical Chemistry and Natural Products Research Unit, Faculty of Pharmacy, Mahasarakham University, Mahasarakham 44150, ThailandDivision of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, ThailandDivision of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand<i>Dipterocarpus alatus</i> (Dipterocarpaceae) is a medicinal plant whose use is well known for the treatment of genito-urinary diseases. However, there is no report of its cytotoxic potential. In this study, the chemical composition, antioxidant and cytotoxic activities of extracts of the leaves, bark, twigs and oleo-resin from <i>D. alatus</i> are investigated. Cytotoxicity was measured by the neutral red (NR) assay against HCT116, SKLU1, SK-MEL2, SiHa and U937 cancer cell lines and antioxidant capacity was evaluated by DPPH, ABTS radical scavenging, and ferric reducing antioxidant power (FRAP) assays. The chemical composition was analyzed by gas chromatography−mass spectrometry (GC-MS). Leaf, bark and twig extracts exhibited stronger antioxidant activity than oleo-resin, with bark extract showing the highest antioxidant activity and the highest total phenolic content. All samples showed more cytotoxic activity against the U937 cell line than HCT116, SKLU1, SK-MEL2 and SiHa cells with oleo-resin being more cytotoxic than melphalan against U937 cells. Chemical composition analysis of oleo-resin by GC-MS showed that the major components were sesquiterpenes, namely α-gurjunene (30.31%), (-)-isoledene (13.69%), alloaromadendrene (3.28%), β-caryophyllene (3.14%), γ-gurjunene (3.14%) and spathulenol (1.11%). The cytotoxic activity of oleo-resin can be attributed to the sesquiterpene content, whereas the cytotoxic and antioxidant activities of leaf, bark and twig extracts correlated to total phenolic content.https://www.mdpi.com/1420-3049/24/17/3083anticancerantioxidantpolyphenolsesquiterpenesGC-MS analysis<i>Dipterocarpus</i> |
| spellingShingle | Chawalit Yongram Bunleu Sungthong Ploenthip Puthongking Natthida Weerapreeyakul Chemical Composition, Antioxidant and Cytotoxicity Activities of Leaves, Bark, Twigs and Oleo-Resin of <i>Dipterocarpus alatus</i> Molecules anticancer antioxidant polyphenol sesquiterpenes GC-MS analysis <i>Dipterocarpus</i> |
| title | Chemical Composition, Antioxidant and Cytotoxicity Activities of Leaves, Bark, Twigs and Oleo-Resin of <i>Dipterocarpus alatus</i> |
| title_full | Chemical Composition, Antioxidant and Cytotoxicity Activities of Leaves, Bark, Twigs and Oleo-Resin of <i>Dipterocarpus alatus</i> |
| title_fullStr | Chemical Composition, Antioxidant and Cytotoxicity Activities of Leaves, Bark, Twigs and Oleo-Resin of <i>Dipterocarpus alatus</i> |
| title_full_unstemmed | Chemical Composition, Antioxidant and Cytotoxicity Activities of Leaves, Bark, Twigs and Oleo-Resin of <i>Dipterocarpus alatus</i> |
| title_short | Chemical Composition, Antioxidant and Cytotoxicity Activities of Leaves, Bark, Twigs and Oleo-Resin of <i>Dipterocarpus alatus</i> |
| title_sort | chemical composition antioxidant and cytotoxicity activities of leaves bark twigs and oleo resin of i dipterocarpus alatus i |
| topic | anticancer antioxidant polyphenol sesquiterpenes GC-MS analysis <i>Dipterocarpus</i> |
| url | https://www.mdpi.com/1420-3049/24/17/3083 |
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