Expression and function of exportin 6 in full-grown and growing porcine oocytes
Exportin 6, which functions specifically in the nuclear export of actin family proteins, has been reported to be absent in immature Xenopus oocytes, which have a huge nucleus containing a large amount of actin. In mammalian oocytes, however, the presence and the function of exportin 6 remain uninves...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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The Society for Reproduction and Development
2019-06-01
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| Series: | The Journal of Reproduction and Development |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jrd/65/5/65_2019-040/_pdf/-char/en |
| _version_ | 1797614763857936384 |
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| author | Asuka ONUMA Yoshie A. FUJIOKA Wataru FUJII Koji SUGIURA Kunihiko NAITO |
| author_facet | Asuka ONUMA Yoshie A. FUJIOKA Wataru FUJII Koji SUGIURA Kunihiko NAITO |
| author_sort | Asuka ONUMA |
| collection | DOAJ |
| description | Exportin 6, which functions specifically in the nuclear export of actin family proteins, has been reported to be absent in immature Xenopus oocytes, which have a huge nucleus containing a large amount of actin. In mammalian oocytes, however, the presence and the function of exportin 6 remain uninvestigated. In this study, we assessed the expression and effects of exportin 6 on meiotic resumption in porcine oocytes after cloning porcine exportin 6 cDNA and carrying out overexpression and expression inhibition by mRNA and antisense RNA injection, respectively. We found for the first time that exportin 6 was expressed in mammalian full-grown germinal-vesicle-stage oocytes and was involved in the nuclear export of actin. In contrast, exportin 6 was absent from the growing oocytes, which are meiotically incompetent and maintain the germinal-vesicle structure in the long term; the regulatory mechanism appeared to be active degradation. We examined the effects of exportin 6 on meiotic resumption of porcine oocytes and noted that its expression did not affect the onset time but increased the rate of germinal vesicle breakdown at 24 h via regulation of the nuclear actin level, which directly influences the physical strength of the germinal-vesicle membrane. Our results suggest that exportin 6 affects the nuclear transport of actin and meiotic resumption in mammalian oocytes. |
| first_indexed | 2024-03-11T07:17:00Z |
| format | Article |
| id | doaj.art-cdd65bf23d7c4da193cfe1a052dfbacb |
| institution | Directory Open Access Journal |
| issn | 0916-8818 1348-4400 |
| language | English |
| last_indexed | 2024-03-11T07:17:00Z |
| publishDate | 2019-06-01 |
| publisher | The Society for Reproduction and Development |
| record_format | Article |
| series | The Journal of Reproduction and Development |
| spelling | doaj.art-cdd65bf23d7c4da193cfe1a052dfbacb2023-11-17T08:14:38ZengThe Society for Reproduction and DevelopmentThe Journal of Reproduction and Development0916-88181348-44002019-06-0165540741210.1262/jrd.2019-040jrdExpression and function of exportin 6 in full-grown and growing porcine oocytesAsuka ONUMA0Yoshie A. FUJIOKA1Wataru FUJII2Koji SUGIURA3Kunihiko NAITO4Department of Animal Resource Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, JapanDepartment of Animal Resource Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, JapanDepartment of Animal Resource Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, JapanDepartment of Animal Resource Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, JapanDepartment of Animal Resource Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, JapanExportin 6, which functions specifically in the nuclear export of actin family proteins, has been reported to be absent in immature Xenopus oocytes, which have a huge nucleus containing a large amount of actin. In mammalian oocytes, however, the presence and the function of exportin 6 remain uninvestigated. In this study, we assessed the expression and effects of exportin 6 on meiotic resumption in porcine oocytes after cloning porcine exportin 6 cDNA and carrying out overexpression and expression inhibition by mRNA and antisense RNA injection, respectively. We found for the first time that exportin 6 was expressed in mammalian full-grown germinal-vesicle-stage oocytes and was involved in the nuclear export of actin. In contrast, exportin 6 was absent from the growing oocytes, which are meiotically incompetent and maintain the germinal-vesicle structure in the long term; the regulatory mechanism appeared to be active degradation. We examined the effects of exportin 6 on meiotic resumption of porcine oocytes and noted that its expression did not affect the onset time but increased the rate of germinal vesicle breakdown at 24 h via regulation of the nuclear actin level, which directly influences the physical strength of the germinal-vesicle membrane. Our results suggest that exportin 6 affects the nuclear transport of actin and meiotic resumption in mammalian oocytes.https://www.jstage.jst.go.jp/article/jrd/65/5/65_2019-040/_pdf/-char/enactinexportin 6meiotic resumptionnuclear exportpig oocyte |
| spellingShingle | Asuka ONUMA Yoshie A. FUJIOKA Wataru FUJII Koji SUGIURA Kunihiko NAITO Expression and function of exportin 6 in full-grown and growing porcine oocytes The Journal of Reproduction and Development actin exportin 6 meiotic resumption nuclear export pig oocyte |
| title | Expression and function of exportin 6 in full-grown and growing porcine oocytes |
| title_full | Expression and function of exportin 6 in full-grown and growing porcine oocytes |
| title_fullStr | Expression and function of exportin 6 in full-grown and growing porcine oocytes |
| title_full_unstemmed | Expression and function of exportin 6 in full-grown and growing porcine oocytes |
| title_short | Expression and function of exportin 6 in full-grown and growing porcine oocytes |
| title_sort | expression and function of exportin 6 in full grown and growing porcine oocytes |
| topic | actin exportin 6 meiotic resumption nuclear export pig oocyte |
| url | https://www.jstage.jst.go.jp/article/jrd/65/5/65_2019-040/_pdf/-char/en |
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