The protective effects of S14G-humanin (HNG) against streptozotocin (STZ)-induced cardiac dysfunction
Excessive oxidative stress, inflammation, and myocardial hypertrophy have been associated with diabetic cardiomyopathy (DCM). S14G-humanin (HNG) is a potent humanin analogue that has demonstrated cytoprotective effects in a variety of cells and tissues. However, the pharmacological function of HNG i...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2021-01-01
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| Series: | Bioengineered |
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| Online Access: | http://dx.doi.org/10.1080/21655979.2021.1964894 |
| _version_ | 1798034005097971712 |
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| author | Xiaopan Chen Chuan Yun Hailong Zheng Xu Chen Qianfei Han Hua Pan Yang Wang Jianghua Zhong |
| author_facet | Xiaopan Chen Chuan Yun Hailong Zheng Xu Chen Qianfei Han Hua Pan Yang Wang Jianghua Zhong |
| author_sort | Xiaopan Chen |
| collection | DOAJ |
| description | Excessive oxidative stress, inflammation, and myocardial hypertrophy have been associated with diabetic cardiomyopathy (DCM). S14G-humanin (HNG) is a potent humanin analogue that has demonstrated cytoprotective effects in a variety of cells and tissues. However, the pharmacological function of HNG in diabetic cardiomyopathy has not yet been reported. In the present study, we investigated the protective effects of HNG against streptozotocin (STZ)-induced cardiac dysfunction in diabetic mice. Myocardial hypertrophy in diabetic mice was determined using Wheat Gem Agglutinin (WGA) staining. The heart function was measured with Echocardiographic imaging. Levels of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) proteins in plasma were measured using enzyme-linked immunosorbent assay (ELISA) kits. Protein expression of Phosphorylated p38/p38 was determined using western blots. We found that HNG treatment attenuated the STZ-induced myocardial hypertrophy and significantly improved heart function. Also, its treatment proved effective as it reduced the levels of several myocardial injury indicators, including creatine kinase-MB (CK-MB), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), and both the cardiac and plasma levels of TNF-α and IL-6, highlighting its effect on the STZ-induced myocardial injury. Lastly, HNG suppressed the activation of the p38/nuclear factor kappa-B (NF-κB) signaling pathway. S14G humanin possesses protective effects against streptozotocin-induced cardiac dysfunction through inhibiting the activation of the p38/NF-κB signaling pathway. |
| first_indexed | 2024-04-11T20:38:15Z |
| format | Article |
| id | doaj.art-cdd72acc42764332af938cf6ddd69f2b |
| institution | Directory Open Access Journal |
| issn | 2165-5979 2165-5987 |
| language | English |
| last_indexed | 2024-04-11T20:38:15Z |
| publishDate | 2021-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Bioengineered |
| spelling | doaj.art-cdd72acc42764332af938cf6ddd69f2b2022-12-22T04:04:18ZengTaylor & Francis GroupBioengineered2165-59792165-59872021-01-011215491550310.1080/21655979.2021.19648941964894The protective effects of S14G-humanin (HNG) against streptozotocin (STZ)-induced cardiac dysfunctionXiaopan Chen0Chuan Yun1Hailong Zheng2Xu Chen3Qianfei Han4Hua Pan5Yang Wang6Jianghua Zhong7The First Affiliated Hospital of Hainan Medical UniversityThe First Affiliated Hospital of Hainan Medical UniversityThe First Affiliated Hospital of Hainan Medical UniversityThe First Affiliated Hospital of Hainan Medical UniversityThe First Affiliated Hospital of Hainan Medical UniversityThe First Affiliated Hospital of Hainan Medical UniversityAffiliated Haikou Hospital Of Xiangya Medical College, Central South UniversityAffiliated Haikou Hospital Of Xiangya Medical College, Central South UniversityExcessive oxidative stress, inflammation, and myocardial hypertrophy have been associated with diabetic cardiomyopathy (DCM). S14G-humanin (HNG) is a potent humanin analogue that has demonstrated cytoprotective effects in a variety of cells and tissues. However, the pharmacological function of HNG in diabetic cardiomyopathy has not yet been reported. In the present study, we investigated the protective effects of HNG against streptozotocin (STZ)-induced cardiac dysfunction in diabetic mice. Myocardial hypertrophy in diabetic mice was determined using Wheat Gem Agglutinin (WGA) staining. The heart function was measured with Echocardiographic imaging. Levels of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) proteins in plasma were measured using enzyme-linked immunosorbent assay (ELISA) kits. Protein expression of Phosphorylated p38/p38 was determined using western blots. We found that HNG treatment attenuated the STZ-induced myocardial hypertrophy and significantly improved heart function. Also, its treatment proved effective as it reduced the levels of several myocardial injury indicators, including creatine kinase-MB (CK-MB), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), and both the cardiac and plasma levels of TNF-α and IL-6, highlighting its effect on the STZ-induced myocardial injury. Lastly, HNG suppressed the activation of the p38/nuclear factor kappa-B (NF-κB) signaling pathway. S14G humanin possesses protective effects against streptozotocin-induced cardiac dysfunction through inhibiting the activation of the p38/NF-κB signaling pathway.http://dx.doi.org/10.1080/21655979.2021.1964894s14g-humaninstreptozotocincardiac dysfunctiondiabetes |
| spellingShingle | Xiaopan Chen Chuan Yun Hailong Zheng Xu Chen Qianfei Han Hua Pan Yang Wang Jianghua Zhong The protective effects of S14G-humanin (HNG) against streptozotocin (STZ)-induced cardiac dysfunction Bioengineered s14g-humanin streptozotocin cardiac dysfunction diabetes |
| title | The protective effects of S14G-humanin (HNG) against streptozotocin (STZ)-induced cardiac dysfunction |
| title_full | The protective effects of S14G-humanin (HNG) against streptozotocin (STZ)-induced cardiac dysfunction |
| title_fullStr | The protective effects of S14G-humanin (HNG) against streptozotocin (STZ)-induced cardiac dysfunction |
| title_full_unstemmed | The protective effects of S14G-humanin (HNG) against streptozotocin (STZ)-induced cardiac dysfunction |
| title_short | The protective effects of S14G-humanin (HNG) against streptozotocin (STZ)-induced cardiac dysfunction |
| title_sort | protective effects of s14g humanin hng against streptozotocin stz induced cardiac dysfunction |
| topic | s14g-humanin streptozotocin cardiac dysfunction diabetes |
| url | http://dx.doi.org/10.1080/21655979.2021.1964894 |
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