Marked variations in gut microbiota and some innate immune responses of fresh water crayfish, marron (Cherax cainii, Austin 2002) fed dietary supplementation of Clostridium butyricum
This study aimed to investigate the effects of Clostridium butyricum as a dietary probiotic supplement in fishmeal based diet on growth, gut microbiota and immune performance of marron (Cherax cainii). Marron were randomly distributed into two different treatment groups, control and probiotic fed gr...
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PeerJ Inc.
2019-08-01
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| author | Md Javed Foysal Thi Thu Thuy Nguyen Md Reaz Chaklader Muhammad A.B. Siddik Chin-Yen Tay Ravi Fotedar Sanjay Kumar Gupta |
| author_facet | Md Javed Foysal Thi Thu Thuy Nguyen Md Reaz Chaklader Muhammad A.B. Siddik Chin-Yen Tay Ravi Fotedar Sanjay Kumar Gupta |
| author_sort | Md Javed Foysal |
| collection | DOAJ |
| description | This study aimed to investigate the effects of Clostridium butyricum as a dietary probiotic supplement in fishmeal based diet on growth, gut microbiota and immune performance of marron (Cherax cainii). Marron were randomly distributed into two different treatment groups, control and probiotic fed group. After 42 days of feeding trial, the results revealed a significant (P < 0.05) increase in growth due to increase in number of moults in marron fed probiotics. The probiotic diet also significantly enhanced the total haemocyte counts (THC), lysozyme activity in the haemolymph and protein content of the tail muscle in marron. Compared to control, the 16S rRNA sequences data demonstrated an enrichment of bacterial diversity in the probiotic fed marron where significant increase of Clostridium abundance was observed. The abundance for crayfish pathogen Vibrio and Aeromonas were found to be significantly reduced post feeding with probiotic diet. Predicted metabolic pathway revealed an increased activity for the metabolism and absorption of carbohydrate, degradation of amino acid, fatty acid and toxic compounds, and biosynthesis of secondary metabolites. C. butyricum supplementation also significantly modulated the expression level of immune-responsive genes of marron post challenged with Vibrio mimicus. The overall results suggest that C. butyricum could be used as dietary probiotic supplement in marron aquaculture. |
| first_indexed | 2024-03-09T06:52:32Z |
| format | Article |
| id | doaj.art-cdd7327d40cc48b1913c4025fbc70503 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:52:32Z |
| publishDate | 2019-08-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
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| spelling | doaj.art-cdd7327d40cc48b1913c4025fbc705032023-12-03T10:25:08ZengPeerJ Inc.PeerJ2167-83592019-08-017e755310.7717/peerj.7553Marked variations in gut microbiota and some innate immune responses of fresh water crayfish, marron (Cherax cainii, Austin 2002) fed dietary supplementation of Clostridium butyricumMd Javed Foysal0Thi Thu Thuy Nguyen1Md Reaz Chaklader2Muhammad A.B. Siddik3Chin-Yen Tay4Ravi Fotedar5Sanjay Kumar Gupta6School of Molecular and Life Sciences, Curtin University, Bentley, Western Australia, AustraliaSchool of Molecular and Life Sciences, Curtin University, Bentley, Western Australia, AustraliaSchool of Molecular and Life Sciences, Curtin University, Bentley, Western Australia, AustraliaSchool of Molecular and Life Sciences, Curtin University, Bentley, Western Australia, AustraliaHelicobacter Research Laboratory, Marshall Centre for Infectious Disease Research and Training, School of Biomedical Sciences, University of Western Australia, Perth, Western Australia, AustraliaSchool of Molecular and Life Sciences, Curtin University, Bentley, Western Australia, AustraliaICAR-Indian Institute of Agricultural Biotechnology, Ranchi, Jharkhand, IndiaThis study aimed to investigate the effects of Clostridium butyricum as a dietary probiotic supplement in fishmeal based diet on growth, gut microbiota and immune performance of marron (Cherax cainii). Marron were randomly distributed into two different treatment groups, control and probiotic fed group. After 42 days of feeding trial, the results revealed a significant (P < 0.05) increase in growth due to increase in number of moults in marron fed probiotics. The probiotic diet also significantly enhanced the total haemocyte counts (THC), lysozyme activity in the haemolymph and protein content of the tail muscle in marron. Compared to control, the 16S rRNA sequences data demonstrated an enrichment of bacterial diversity in the probiotic fed marron where significant increase of Clostridium abundance was observed. The abundance for crayfish pathogen Vibrio and Aeromonas were found to be significantly reduced post feeding with probiotic diet. Predicted metabolic pathway revealed an increased activity for the metabolism and absorption of carbohydrate, degradation of amino acid, fatty acid and toxic compounds, and biosynthesis of secondary metabolites. C. butyricum supplementation also significantly modulated the expression level of immune-responsive genes of marron post challenged with Vibrio mimicus. The overall results suggest that C. butyricum could be used as dietary probiotic supplement in marron aquaculture.https://peerj.com/articles/7553.pdfMarronProbiotic bacteriaHealth and immune indicesHigh throughput sequencingBioinformaticsGene expression profiling |
| spellingShingle | Md Javed Foysal Thi Thu Thuy Nguyen Md Reaz Chaklader Muhammad A.B. Siddik Chin-Yen Tay Ravi Fotedar Sanjay Kumar Gupta Marked variations in gut microbiota and some innate immune responses of fresh water crayfish, marron (Cherax cainii, Austin 2002) fed dietary supplementation of Clostridium butyricum PeerJ Marron Probiotic bacteria Health and immune indices High throughput sequencing Bioinformatics Gene expression profiling |
| title | Marked variations in gut microbiota and some innate immune responses of fresh water crayfish, marron (Cherax cainii, Austin 2002) fed dietary supplementation of Clostridium butyricum |
| title_full | Marked variations in gut microbiota and some innate immune responses of fresh water crayfish, marron (Cherax cainii, Austin 2002) fed dietary supplementation of Clostridium butyricum |
| title_fullStr | Marked variations in gut microbiota and some innate immune responses of fresh water crayfish, marron (Cherax cainii, Austin 2002) fed dietary supplementation of Clostridium butyricum |
| title_full_unstemmed | Marked variations in gut microbiota and some innate immune responses of fresh water crayfish, marron (Cherax cainii, Austin 2002) fed dietary supplementation of Clostridium butyricum |
| title_short | Marked variations in gut microbiota and some innate immune responses of fresh water crayfish, marron (Cherax cainii, Austin 2002) fed dietary supplementation of Clostridium butyricum |
| title_sort | marked variations in gut microbiota and some innate immune responses of fresh water crayfish marron cherax cainii austin 2002 fed dietary supplementation of clostridium butyricum |
| topic | Marron Probiotic bacteria Health and immune indices High throughput sequencing Bioinformatics Gene expression profiling |
| url | https://peerj.com/articles/7553.pdf |
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