A Molecular Study on Hepatitis E Virus (HEV) in Pigs in Bulgaria
Information on hepatitis E virus (HEV) strains circulating in animal reservoirs in Bulgaria is currently lacking. Herein, by screening HEV seropositive sera obtained from Bulgarian swine and wild boars, viral RNA was detected at high prevalence rate (28.2%) in industrial pigs. Sequence analysis of t...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-11-01
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| Series: | Veterinary Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2306-7381/8/11/267 |
| _version_ | 1797508271158853632 |
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| author | Andrea Palombieri Ilia Tsachev Vittorio Sarchese Paola Fruci Federica Di Profio Roman Pepovich Magdalena Baymakova Fulvio Marsilio Vito Martella Barbara Di Martino |
| author_facet | Andrea Palombieri Ilia Tsachev Vittorio Sarchese Paola Fruci Federica Di Profio Roman Pepovich Magdalena Baymakova Fulvio Marsilio Vito Martella Barbara Di Martino |
| author_sort | Andrea Palombieri |
| collection | DOAJ |
| description | Information on hepatitis E virus (HEV) strains circulating in animal reservoirs in Bulgaria is currently lacking. Herein, by screening HEV seropositive sera obtained from Bulgarian swine and wild boars, viral RNA was detected at high prevalence rate (28.2%) in industrial pigs. Sequence analysis of the partial polymerase (RdRp) region revealed the highest genetic correlation with HEVs of genotype (Gt) 3 identified in French and Dutch patients. For three such strains, a 700-bp fragment of the open reading frame 2 gene was generated. On phylogenetic analysis, the Bulgarian strains clustered tightly (93.8–98.3% nt) with human and animal HEVs classified within the Gt3 subtype c. |
| first_indexed | 2024-03-10T04:59:34Z |
| format | Article |
| id | doaj.art-cddd4d1789f5494e896808d12b8b5034 |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-10T04:59:34Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-cddd4d1789f5494e896808d12b8b50342023-11-23T01:54:30ZengMDPI AGVeterinary Sciences2306-73812021-11-0181126710.3390/vetsci8110267A Molecular Study on Hepatitis E Virus (HEV) in Pigs in BulgariaAndrea Palombieri0Ilia Tsachev1Vittorio Sarchese2Paola Fruci3Federica Di Profio4Roman Pepovich5Magdalena Baymakova6Fulvio Marsilio7Vito Martella8Barbara Di Martino9Faculty of Veterinary Medicine, Università degli Studi di Teramo, 64100 Teramo, ItalyDepartment of Microbiology, Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, Trakia University, 6000 Stara Zagora, BulgariaFaculty of Veterinary Medicine, Università degli Studi di Teramo, 64100 Teramo, ItalyFaculty of Veterinary Medicine, Università degli Studi di Teramo, 64100 Teramo, ItalyFaculty of Veterinary Medicine, Università degli Studi di Teramo, 64100 Teramo, ItalyDepartment of Infectious Pathology, Hygiene, Technology and Control of Foods from Animal Origin, Faculty of Veterinary Medicine, University of Forestry, 1797 Sofia, BulgariaDepartment of Infectious Diseases, Military Medical Academy, 1606 Sofia, BulgariaFaculty of Veterinary Medicine, Università degli Studi di Teramo, 64100 Teramo, ItalyDepartment of Veterinary Medicine, Università Aldo Moro di Bari, 70010 Valenzano, ItalyFaculty of Veterinary Medicine, Università degli Studi di Teramo, 64100 Teramo, ItalyInformation on hepatitis E virus (HEV) strains circulating in animal reservoirs in Bulgaria is currently lacking. Herein, by screening HEV seropositive sera obtained from Bulgarian swine and wild boars, viral RNA was detected at high prevalence rate (28.2%) in industrial pigs. Sequence analysis of the partial polymerase (RdRp) region revealed the highest genetic correlation with HEVs of genotype (Gt) 3 identified in French and Dutch patients. For three such strains, a 700-bp fragment of the open reading frame 2 gene was generated. On phylogenetic analysis, the Bulgarian strains clustered tightly (93.8–98.3% nt) with human and animal HEVs classified within the Gt3 subtype c.https://www.mdpi.com/2306-7381/8/11/267hepatitis E virus (HEV)RNAgenotype 3pigsBulgaria |
| spellingShingle | Andrea Palombieri Ilia Tsachev Vittorio Sarchese Paola Fruci Federica Di Profio Roman Pepovich Magdalena Baymakova Fulvio Marsilio Vito Martella Barbara Di Martino A Molecular Study on Hepatitis E Virus (HEV) in Pigs in Bulgaria Veterinary Sciences hepatitis E virus (HEV) RNA genotype 3 pigs Bulgaria |
| title | A Molecular Study on Hepatitis E Virus (HEV) in Pigs in Bulgaria |
| title_full | A Molecular Study on Hepatitis E Virus (HEV) in Pigs in Bulgaria |
| title_fullStr | A Molecular Study on Hepatitis E Virus (HEV) in Pigs in Bulgaria |
| title_full_unstemmed | A Molecular Study on Hepatitis E Virus (HEV) in Pigs in Bulgaria |
| title_short | A Molecular Study on Hepatitis E Virus (HEV) in Pigs in Bulgaria |
| title_sort | molecular study on hepatitis e virus hev in pigs in bulgaria |
| topic | hepatitis E virus (HEV) RNA genotype 3 pigs Bulgaria |
| url | https://www.mdpi.com/2306-7381/8/11/267 |
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