Telomere attrition in heart failure: a flow-FISH longitudinal analysis of circulating monocytes
Abstract Background Cross-sectional investigations report shorter telomeres in patients with heart failure (HF); however, no studies describe telomere length (TL) trajectory and its relationship with HF progression. Here we aimed to investigate telomere shortening over time and its relationship to o...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-02-01
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| Series: | Journal of Translational Medicine |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12967-018-1412-z |
| _version_ | 1819090331088453632 |
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| author | Iris Teubel Elena Elchinova Santiago Roura Marco A. Fernández Carolina Gálvez-Montón Pedro Moliner Marta de Antonio Josep Lupón Antoni Bayés-Genís |
| author_facet | Iris Teubel Elena Elchinova Santiago Roura Marco A. Fernández Carolina Gálvez-Montón Pedro Moliner Marta de Antonio Josep Lupón Antoni Bayés-Genís |
| author_sort | Iris Teubel |
| collection | DOAJ |
| description | Abstract Background Cross-sectional investigations report shorter telomeres in patients with heart failure (HF); however, no studies describe telomere length (TL) trajectory and its relationship with HF progression. Here we aimed to investigate telomere shortening over time and its relationship to outcomes. Methods Our study cohort included 101 ambulatory patients with HF. Blood samples were collected at baseline (n = 101) and at the 1-year follow-up (n = 54). Using flow-FISH analysis of circulating monocytes, we simultaneously measured three monocyte subsets—classical (CD14++CD16−), intermediate (CD14++CD16+), and nonclassical (CD14+CD16++)—and their respective TLs based on FITC-labeled PNA probe hybridization. The primary endpoints were all-cause death and the composite of all-cause death or HF-related hospitalization, assessed at 2.3 ± 0.6 years. All statistical analyses were executed by using the SPSS 15.0 software, and included Student’s t test and ANOVA with post hoc Scheffe analysis, Pearson or Spearman rho correlation and univariate Cox regression when applicable. Results We found high correlations between TL values of different monocyte subsets: CD14++CD16+ vs. CD14++CD16−, R = 0.95, p < 0.001; CD14++CD16+ vs. CD14+CD16++, R = 0.90, p < 0.001; and CD14++CD16− vs. CD14+CD16++, R = 0.89, p < 0.001. Mean monocyte TL exhibited significant attrition from baseline to the 1-year follow-up (11.1 ± 3.3 vs. 8.3 ± 2.1, p < 0.001). TL did not significantly differ between monocyte subsets at either sampling time-point (all p values > 0.1). Cox regression analyses did not indicate that TL or ΔTL was associated with all-cause death or the composite endpoint. Conclusions Overall, this longitudinal study demonstrated a ~ 22% reduction of TL in monocytes from ambulatory patients with HF within 1 year. TL and ΔTL were not related to outcomes over long-term follow-up. |
| first_indexed | 2024-12-21T22:22:07Z |
| format | Article |
| id | doaj.art-cdde35347e614a5eb86f06bb3f09dd66 |
| institution | Directory Open Access Journal |
| issn | 1479-5876 |
| language | English |
| last_indexed | 2024-12-21T22:22:07Z |
| publishDate | 2018-02-01 |
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| series | Journal of Translational Medicine |
| spelling | doaj.art-cdde35347e614a5eb86f06bb3f09dd662022-12-21T18:48:18ZengBMCJournal of Translational Medicine1479-58762018-02-011611810.1186/s12967-018-1412-zTelomere attrition in heart failure: a flow-FISH longitudinal analysis of circulating monocytesIris Teubel0Elena Elchinova1Santiago Roura2Marco A. Fernández3Carolina Gálvez-Montón4Pedro Moliner5Marta de Antonio6Josep Lupón7Antoni Bayés-Genís8Flow Cytometry Facility, Germans Trias i Pujol Health Science Research InstituteCardiology Service, Germans Trias i Pujol University HospitalICREC Research Program, Germans Trias i Pujol Health Science Research InstituteFlow Cytometry Facility, Germans Trias i Pujol Health Science Research InstituteICREC Research Program, Germans Trias i Pujol Health Science Research InstituteCardiology Service, Germans Trias i Pujol University HospitalCardiology Service, Germans Trias i Pujol University HospitalCardiology Service, Germans Trias i Pujol University HospitalCardiology Service, Germans Trias i Pujol University HospitalAbstract Background Cross-sectional investigations report shorter telomeres in patients with heart failure (HF); however, no studies describe telomere length (TL) trajectory and its relationship with HF progression. Here we aimed to investigate telomere shortening over time and its relationship to outcomes. Methods Our study cohort included 101 ambulatory patients with HF. Blood samples were collected at baseline (n = 101) and at the 1-year follow-up (n = 54). Using flow-FISH analysis of circulating monocytes, we simultaneously measured three monocyte subsets—classical (CD14++CD16−), intermediate (CD14++CD16+), and nonclassical (CD14+CD16++)—and their respective TLs based on FITC-labeled PNA probe hybridization. The primary endpoints were all-cause death and the composite of all-cause death or HF-related hospitalization, assessed at 2.3 ± 0.6 years. All statistical analyses were executed by using the SPSS 15.0 software, and included Student’s t test and ANOVA with post hoc Scheffe analysis, Pearson or Spearman rho correlation and univariate Cox regression when applicable. Results We found high correlations between TL values of different monocyte subsets: CD14++CD16+ vs. CD14++CD16−, R = 0.95, p < 0.001; CD14++CD16+ vs. CD14+CD16++, R = 0.90, p < 0.001; and CD14++CD16− vs. CD14+CD16++, R = 0.89, p < 0.001. Mean monocyte TL exhibited significant attrition from baseline to the 1-year follow-up (11.1 ± 3.3 vs. 8.3 ± 2.1, p < 0.001). TL did not significantly differ between monocyte subsets at either sampling time-point (all p values > 0.1). Cox regression analyses did not indicate that TL or ΔTL was associated with all-cause death or the composite endpoint. Conclusions Overall, this longitudinal study demonstrated a ~ 22% reduction of TL in monocytes from ambulatory patients with HF within 1 year. TL and ΔTL were not related to outcomes over long-term follow-up.http://link.springer.com/article/10.1186/s12967-018-1412-zHeart failureMonocyte subsetsTelomere attritionTelomere length |
| spellingShingle | Iris Teubel Elena Elchinova Santiago Roura Marco A. Fernández Carolina Gálvez-Montón Pedro Moliner Marta de Antonio Josep Lupón Antoni Bayés-Genís Telomere attrition in heart failure: a flow-FISH longitudinal analysis of circulating monocytes Journal of Translational Medicine Heart failure Monocyte subsets Telomere attrition Telomere length |
| title | Telomere attrition in heart failure: a flow-FISH longitudinal analysis of circulating monocytes |
| title_full | Telomere attrition in heart failure: a flow-FISH longitudinal analysis of circulating monocytes |
| title_fullStr | Telomere attrition in heart failure: a flow-FISH longitudinal analysis of circulating monocytes |
| title_full_unstemmed | Telomere attrition in heart failure: a flow-FISH longitudinal analysis of circulating monocytes |
| title_short | Telomere attrition in heart failure: a flow-FISH longitudinal analysis of circulating monocytes |
| title_sort | telomere attrition in heart failure a flow fish longitudinal analysis of circulating monocytes |
| topic | Heart failure Monocyte subsets Telomere attrition Telomere length |
| url | http://link.springer.com/article/10.1186/s12967-018-1412-z |
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