Antiviral Decoction of Isatidis Radix (板藍根 bǎn lán gēn) Inhibited Influenza Virus Adsorption on MDCK Cells by Cytoprotective Activity
The aim of this study is to elucidate how the Isatidis Radix (板藍根 bǎn lán gēn) tonic, as an aqueous mixture of hundreds of compositions, interrupts the infection of influenza viruses to their host cells. The efficacy of the tonic was evaluated and expressed as cell proliferation rate and plaque redu...
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| Format: | Article |
| Language: | English |
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Elsevier
2012-01-01
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| Series: | Journal of Traditional and Complementary Medicine |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2225411016300700 |
| _version_ | 1818544453617451008 |
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| author | Lijing Ke Teng Wen Jeremy P. Bradshaw Jianwu Zhou Pingfan Rao |
| author_facet | Lijing Ke Teng Wen Jeremy P. Bradshaw Jianwu Zhou Pingfan Rao |
| author_sort | Lijing Ke |
| collection | DOAJ |
| description | The aim of this study is to elucidate how the Isatidis Radix (板藍根 bǎn lán gēn) tonic, as an aqueous mixture of hundreds of compositions, interrupts the infection of influenza viruses to their host cells. The efficacy of the tonic was evaluated and expressed as cell proliferation rate and plaque reduction rate in Madin-Darby Canine Kidney (MDCK) cells, against 3 strains of influenza A and B viruses. This boiling water (at 100 °C) extract of Isatidis Radix (RIE) showed antiviral activity against influenza virus A and B. The concentration for 50% inhibition of influenza virus A replication (IC50) in MDCK cell was 12.6 mg/mL with a therapeutic index >8. When cells were incubated with RIE prior to virus adsorption, the numbers of viable cell were at least doubled compared to the numbers of virus control, RIE incubation after virus adsorption and RIE incubation with virus prior to adsorption, in both influenza virus A and B. Moreover, much less virus particles were spotted by scanning electron microscope (SEM) in the RIE pre-treated cells than the cells without RIE treatment. These results indicate the antiviral activity of RIE is mainly attributed to its host cell protection effect but not actions on virus or post-virus-adsorption interruption. Cell, but not virus, is more likely to be the action target of RIE. |
| first_indexed | 2024-12-11T22:48:42Z |
| format | Article |
| id | doaj.art-cde026cf0c94464dba656dd40d6fa57f |
| institution | Directory Open Access Journal |
| issn | 2225-4110 |
| language | English |
| last_indexed | 2024-12-11T22:48:42Z |
| publishDate | 2012-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Traditional and Complementary Medicine |
| spelling | doaj.art-cde026cf0c94464dba656dd40d6fa57f2022-12-22T00:47:32ZengElsevierJournal of Traditional and Complementary Medicine2225-41102012-01-0121475110.1016/S2225-4110(16)30070-0Antiviral Decoction of Isatidis Radix (板藍根 bǎn lán gēn) Inhibited Influenza Virus Adsorption on MDCK Cells by Cytoprotective ActivityLijing Ke0Teng Wen1Jeremy P. Bradshaw2Jianwu Zhou3Pingfan Rao4Institute of Biotechnology, Fuzhou University, Fuzhou, Fujian, 350002, P.R.ChinaInstitute of Biotechnology, Fuzhou University, Fuzhou, Fujian, 350002, P.R.ChinaRoyal (Dick) School of Veterinary Studies, The University of Edinburgh, Edinburgh, EH9 1QH, U.K.Institute of Biotechnology, Fuzhou University, Fuzhou, Fujian, 350002, P.R.ChinaInstitute of Biotechnology, Fuzhou University, Fuzhou, Fujian, 350002, P.R.ChinaThe aim of this study is to elucidate how the Isatidis Radix (板藍根 bǎn lán gēn) tonic, as an aqueous mixture of hundreds of compositions, interrupts the infection of influenza viruses to their host cells. The efficacy of the tonic was evaluated and expressed as cell proliferation rate and plaque reduction rate in Madin-Darby Canine Kidney (MDCK) cells, against 3 strains of influenza A and B viruses. This boiling water (at 100 °C) extract of Isatidis Radix (RIE) showed antiviral activity against influenza virus A and B. The concentration for 50% inhibition of influenza virus A replication (IC50) in MDCK cell was 12.6 mg/mL with a therapeutic index >8. When cells were incubated with RIE prior to virus adsorption, the numbers of viable cell were at least doubled compared to the numbers of virus control, RIE incubation after virus adsorption and RIE incubation with virus prior to adsorption, in both influenza virus A and B. Moreover, much less virus particles were spotted by scanning electron microscope (SEM) in the RIE pre-treated cells than the cells without RIE treatment. These results indicate the antiviral activity of RIE is mainly attributed to its host cell protection effect but not actions on virus or post-virus-adsorption interruption. Cell, but not virus, is more likely to be the action target of RIE.http://www.sciencedirect.com/science/article/pii/S2225411016300700Isatidis Radix (板藍根 bǎn lán gēn)Antiviral effectsCell bindingInfluenza virusCell protection |
| spellingShingle | Lijing Ke Teng Wen Jeremy P. Bradshaw Jianwu Zhou Pingfan Rao Antiviral Decoction of Isatidis Radix (板藍根 bǎn lán gēn) Inhibited Influenza Virus Adsorption on MDCK Cells by Cytoprotective Activity Journal of Traditional and Complementary Medicine Isatidis Radix (板藍根 bǎn lán gēn) Antiviral effects Cell binding Influenza virus Cell protection |
| title | Antiviral Decoction of Isatidis Radix (板藍根 bǎn lán gēn) Inhibited Influenza Virus Adsorption on MDCK Cells by Cytoprotective Activity |
| title_full | Antiviral Decoction of Isatidis Radix (板藍根 bǎn lán gēn) Inhibited Influenza Virus Adsorption on MDCK Cells by Cytoprotective Activity |
| title_fullStr | Antiviral Decoction of Isatidis Radix (板藍根 bǎn lán gēn) Inhibited Influenza Virus Adsorption on MDCK Cells by Cytoprotective Activity |
| title_full_unstemmed | Antiviral Decoction of Isatidis Radix (板藍根 bǎn lán gēn) Inhibited Influenza Virus Adsorption on MDCK Cells by Cytoprotective Activity |
| title_short | Antiviral Decoction of Isatidis Radix (板藍根 bǎn lán gēn) Inhibited Influenza Virus Adsorption on MDCK Cells by Cytoprotective Activity |
| title_sort | antiviral decoction of isatidis radix 板藍根 ban lan gen inhibited influenza virus adsorption on mdck cells by cytoprotective activity |
| topic | Isatidis Radix (板藍根 bǎn lán gēn) Antiviral effects Cell binding Influenza virus Cell protection |
| url | http://www.sciencedirect.com/science/article/pii/S2225411016300700 |
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