The impact of Allgower-Donati suture pattern and postoperative sweet foods on wound suture breakage in experimental rats
Background: Wound gnawing and/or scratching in rats often occurs in experimental models, causing suture breakage and wound dehiscence, and consequently affecting experimental results and wasting resources. This study aimed to investigate the impact of the combined postoperative use of the Allgower-D...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-03-01
|
| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023011416 |
| _version_ | 1797851940022910976 |
|---|---|
| author | Weifeng Li Feng Xiong Cheng Yao Tingbao Zhang Liangshuang Zhou Zhanyue Zhang Zhaodong Wang Yingji Mao Pinghui Zhou Jianzhong Guan |
| author_facet | Weifeng Li Feng Xiong Cheng Yao Tingbao Zhang Liangshuang Zhou Zhanyue Zhang Zhaodong Wang Yingji Mao Pinghui Zhou Jianzhong Guan |
| author_sort | Weifeng Li |
| collection | DOAJ |
| description | Background: Wound gnawing and/or scratching in rats often occurs in experimental models, causing suture breakage and wound dehiscence, and consequently affecting experimental results and wasting resources. This study aimed to investigate the impact of the combined postoperative use of the Allgower-Donati (A-D) suture pattern and sweet foods on suture breakage, inflammation, and healing in wounds. Materials and methods: Sprague Dawley (SD) rats (n = 48) were treated for linear wounds on the back by four procedures: simple suture, simple suture with postoperative sweet foods, A-D suture, and A-D suture with postoperative sweet foods. Additionally, CD68 immunofluorescence and CD31 immunohistochemistry were used to analyze wound inflammation and vascularization, respectively, on postoperative day 7. Sirius red staining was used to assess collagen deposition on postoperative day 14. Results: Gnawing and scratching of wound sutures were significantly reduced in treated rats (P < 0.01). Neovascularization and collagen deposition were significantly increased (P < 0.001), and inflammatory responses were significantly reduced (P < 0.001) in animals receiving AD sutures and postoperative sweet foods. CD31/CD68 analyses showed that A-D suture and postoperative sweet foods regulated wound angiogenesis and attenuated wound inflammation. Conclusions: Sweet food provision after A-D suture union surgery could reduce wound gnawing and/or scratching, suture breakage, incisional dehiscence, wound inflammation, and promote wound healing in rats. |
| first_indexed | 2024-04-09T19:24:56Z |
| format | Article |
| id | doaj.art-cde3d9d013bd4731a5b23a79d26b1a48 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-09T19:24:56Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-cde3d9d013bd4731a5b23a79d26b1a482023-04-05T08:19:26ZengElsevierHeliyon2405-84402023-03-0193e13934The impact of Allgower-Donati suture pattern and postoperative sweet foods on wound suture breakage in experimental ratsWeifeng Li0Feng Xiong1Cheng Yao2Tingbao Zhang3Liangshuang Zhou4Zhanyue Zhang5Zhaodong Wang6Yingji Mao7Pinghui Zhou8Jianzhong Guan9Department of Orthopedics, First Affiliated Hospital, Bengbu Medical College, Bengbu, China; First Department of Orthopedics, People's Hospital of Lixin County, Bozhou, China; Anhui Province Key Laboratory of Tissue Transplantation, Bengbu, ChinaDepartment of Orthopedics, First Affiliated Hospital, Bengbu Medical College, Bengbu, ChinaDepartment of Orthopedics, First Affiliated Hospital, Bengbu Medical College, Bengbu, ChinaDepartment of Orthopedics, First Affiliated Hospital, Bengbu Medical College, Bengbu, ChinaDepartment of Orthopedics, First Affiliated Hospital, Bengbu Medical College, Bengbu, ChinaDepartment of Orthopedics, First Affiliated Hospital, Bengbu Medical College, Bengbu, China; Anhui Province Key Laboratory of Tissue Transplantation, Bengbu, ChinaDepartment of Orthopedics, First Affiliated Hospital, Bengbu Medical College, Bengbu, China; Anhui Province Key Laboratory of Tissue Transplantation, Bengbu, ChinaAnhui Province Key Laboratory of Tissue Transplantation, Bengbu, ChinaDepartment of Orthopedics, First Affiliated Hospital, Bengbu Medical College, Bengbu, China; Anhui Province Key Laboratory of Tissue Transplantation, Bengbu, ChinaDepartment of Orthopedics, First Affiliated Hospital, Bengbu Medical College, Bengbu, China; Anhui Province Key Laboratory of Tissue Transplantation, Bengbu, China; Corresponding author. Department of Orthopedics, First Affiliated Hospital, Bengbu Medical College, Bengbu, China.Background: Wound gnawing and/or scratching in rats often occurs in experimental models, causing suture breakage and wound dehiscence, and consequently affecting experimental results and wasting resources. This study aimed to investigate the impact of the combined postoperative use of the Allgower-Donati (A-D) suture pattern and sweet foods on suture breakage, inflammation, and healing in wounds. Materials and methods: Sprague Dawley (SD) rats (n = 48) were treated for linear wounds on the back by four procedures: simple suture, simple suture with postoperative sweet foods, A-D suture, and A-D suture with postoperative sweet foods. Additionally, CD68 immunofluorescence and CD31 immunohistochemistry were used to analyze wound inflammation and vascularization, respectively, on postoperative day 7. Sirius red staining was used to assess collagen deposition on postoperative day 14. Results: Gnawing and scratching of wound sutures were significantly reduced in treated rats (P < 0.01). Neovascularization and collagen deposition were significantly increased (P < 0.001), and inflammatory responses were significantly reduced (P < 0.001) in animals receiving AD sutures and postoperative sweet foods. CD31/CD68 analyses showed that A-D suture and postoperative sweet foods regulated wound angiogenesis and attenuated wound inflammation. Conclusions: Sweet food provision after A-D suture union surgery could reduce wound gnawing and/or scratching, suture breakage, incisional dehiscence, wound inflammation, and promote wound healing in rats.http://www.sciencedirect.com/science/article/pii/S2405844023011416Experimental ratsAllgower-Donati suture patternSweet foodWound careWound suture breakageWound healing |
| spellingShingle | Weifeng Li Feng Xiong Cheng Yao Tingbao Zhang Liangshuang Zhou Zhanyue Zhang Zhaodong Wang Yingji Mao Pinghui Zhou Jianzhong Guan The impact of Allgower-Donati suture pattern and postoperative sweet foods on wound suture breakage in experimental rats Heliyon Experimental rats Allgower-Donati suture pattern Sweet food Wound care Wound suture breakage Wound healing |
| title | The impact of Allgower-Donati suture pattern and postoperative sweet foods on wound suture breakage in experimental rats |
| title_full | The impact of Allgower-Donati suture pattern and postoperative sweet foods on wound suture breakage in experimental rats |
| title_fullStr | The impact of Allgower-Donati suture pattern and postoperative sweet foods on wound suture breakage in experimental rats |
| title_full_unstemmed | The impact of Allgower-Donati suture pattern and postoperative sweet foods on wound suture breakage in experimental rats |
| title_short | The impact of Allgower-Donati suture pattern and postoperative sweet foods on wound suture breakage in experimental rats |
| title_sort | impact of allgower donati suture pattern and postoperative sweet foods on wound suture breakage in experimental rats |
| topic | Experimental rats Allgower-Donati suture pattern Sweet food Wound care Wound suture breakage Wound healing |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023011416 |
| work_keys_str_mv | AT weifengli theimpactofallgowerdonatisuturepatternandpostoperativesweetfoodsonwoundsuturebreakageinexperimentalrats AT fengxiong theimpactofallgowerdonatisuturepatternandpostoperativesweetfoodsonwoundsuturebreakageinexperimentalrats AT chengyao theimpactofallgowerdonatisuturepatternandpostoperativesweetfoodsonwoundsuturebreakageinexperimentalrats AT tingbaozhang theimpactofallgowerdonatisuturepatternandpostoperativesweetfoodsonwoundsuturebreakageinexperimentalrats AT liangshuangzhou theimpactofallgowerdonatisuturepatternandpostoperativesweetfoodsonwoundsuturebreakageinexperimentalrats AT zhanyuezhang theimpactofallgowerdonatisuturepatternandpostoperativesweetfoodsonwoundsuturebreakageinexperimentalrats AT zhaodongwang theimpactofallgowerdonatisuturepatternandpostoperativesweetfoodsonwoundsuturebreakageinexperimentalrats AT yingjimao theimpactofallgowerdonatisuturepatternandpostoperativesweetfoodsonwoundsuturebreakageinexperimentalrats AT pinghuizhou theimpactofallgowerdonatisuturepatternandpostoperativesweetfoodsonwoundsuturebreakageinexperimentalrats AT jianzhongguan theimpactofallgowerdonatisuturepatternandpostoperativesweetfoodsonwoundsuturebreakageinexperimentalrats AT weifengli impactofallgowerdonatisuturepatternandpostoperativesweetfoodsonwoundsuturebreakageinexperimentalrats AT fengxiong impactofallgowerdonatisuturepatternandpostoperativesweetfoodsonwoundsuturebreakageinexperimentalrats AT chengyao impactofallgowerdonatisuturepatternandpostoperativesweetfoodsonwoundsuturebreakageinexperimentalrats AT tingbaozhang impactofallgowerdonatisuturepatternandpostoperativesweetfoodsonwoundsuturebreakageinexperimentalrats AT liangshuangzhou impactofallgowerdonatisuturepatternandpostoperativesweetfoodsonwoundsuturebreakageinexperimentalrats AT zhanyuezhang impactofallgowerdonatisuturepatternandpostoperativesweetfoodsonwoundsuturebreakageinexperimentalrats AT zhaodongwang impactofallgowerdonatisuturepatternandpostoperativesweetfoodsonwoundsuturebreakageinexperimentalrats AT yingjimao impactofallgowerdonatisuturepatternandpostoperativesweetfoodsonwoundsuturebreakageinexperimentalrats AT pinghuizhou impactofallgowerdonatisuturepatternandpostoperativesweetfoodsonwoundsuturebreakageinexperimentalrats AT jianzhongguan impactofallgowerdonatisuturepatternandpostoperativesweetfoodsonwoundsuturebreakageinexperimentalrats |