Bioremediation of Neonicotinoid Pesticide, Imidacloprid, Mediated by <i>Bacillus cereus</i>
Imidacloprid, a toxic pesticide of the chloronicotinyl category, is employed extensively in agricultural fields, and its exposure causes serious health issues. Biodegradation is considered to be a green and economical approach to remediate pesticides. Herein, imidacloprid degradation efficiency of &...
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| Format: | Article |
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MDPI AG
2023-08-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/10/8/951 |
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| author | Farah Naz Talpur Ahsanullah Unar Sana Kanwal Bhatti Laila Alsawalha Dalia Fouad Humaira Bashir Hassan Imran Afridi Farid Shokry Ataya Ohoud A. Jefri Muhammad Sohail Bashir |
| author_facet | Farah Naz Talpur Ahsanullah Unar Sana Kanwal Bhatti Laila Alsawalha Dalia Fouad Humaira Bashir Hassan Imran Afridi Farid Shokry Ataya Ohoud A. Jefri Muhammad Sohail Bashir |
| author_sort | Farah Naz Talpur |
| collection | DOAJ |
| description | Imidacloprid, a toxic pesticide of the chloronicotinyl category, is employed extensively in agricultural fields, and its exposure causes serious health issues. Biodegradation is considered to be a green and economical approach to remediate pesticides. Herein, imidacloprid degradation efficiency of <i>Bacillus</i> sp. is highlighted, among which <i>Bacillus cereus</i> exhibited the greatest degradation; optimization of experimental variables (pH, imidacloprid and agitation time) via Box–Behnken factorial design and analysis of variance (ANOVA) revealed 92% biodegradation at the initial substrate concentration of 0.03 mM, aerobically in 11 days under favorable pH 7. The subsequent metabolites, identified through liquid chromatography–mass spectrometry, were 5-hydroxy imidacloprid, imidacloprid-guanidine and 6-chloronicotinic acid. |
| first_indexed | 2024-03-11T00:06:54Z |
| format | Article |
| id | doaj.art-cde57d9d535c49408267187507a2a885 |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-11T00:06:54Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-cde57d9d535c49408267187507a2a8852023-11-19T00:18:17ZengMDPI AGBioengineering2306-53542023-08-0110895110.3390/bioengineering10080951Bioremediation of Neonicotinoid Pesticide, Imidacloprid, Mediated by <i>Bacillus cereus</i>Farah Naz Talpur0Ahsanullah Unar1Sana Kanwal Bhatti2Laila Alsawalha3Dalia Fouad4Humaira Bashir5Hassan Imran Afridi6Farid Shokry Ataya7Ohoud A. Jefri8Muhammad Sohail Bashir9National Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro 76080, PakistanSchool of Life Sciences, University of Sciences and Technology of China, Hefei 230052, ChinaNational Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro 76080, PakistanFaculty of Science, Zarqa University, Zarqa 13110, JordanDepartment of Zoology, College of Science, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi ArabiaDepartment of Botany, University of the Punjab, Quaid-e-Azam Campus, 54590 Lahore, PakistanNational Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro 76080, PakistanDepartment of Biochemistry, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaDepartment of Biological Science, Faculty of Science, King Abdul-Aziz University, Jeddah 2158, Saudi ArabiaInstitutes of Physical Science and Information Technology, Key Laboratory of Structure and Functional Regulation of Hybrid Materials of Ministry of Education, Anhui University, Hefei 230601, ChinaImidacloprid, a toxic pesticide of the chloronicotinyl category, is employed extensively in agricultural fields, and its exposure causes serious health issues. Biodegradation is considered to be a green and economical approach to remediate pesticides. Herein, imidacloprid degradation efficiency of <i>Bacillus</i> sp. is highlighted, among which <i>Bacillus cereus</i> exhibited the greatest degradation; optimization of experimental variables (pH, imidacloprid and agitation time) via Box–Behnken factorial design and analysis of variance (ANOVA) revealed 92% biodegradation at the initial substrate concentration of 0.03 mM, aerobically in 11 days under favorable pH 7. The subsequent metabolites, identified through liquid chromatography–mass spectrometry, were 5-hydroxy imidacloprid, imidacloprid-guanidine and 6-chloronicotinic acid.https://www.mdpi.com/2306-5354/10/8/951imidacloprid<i>Bacillus cereus</i>degradationmetabolites |
| spellingShingle | Farah Naz Talpur Ahsanullah Unar Sana Kanwal Bhatti Laila Alsawalha Dalia Fouad Humaira Bashir Hassan Imran Afridi Farid Shokry Ataya Ohoud A. Jefri Muhammad Sohail Bashir Bioremediation of Neonicotinoid Pesticide, Imidacloprid, Mediated by <i>Bacillus cereus</i> Bioengineering imidacloprid <i>Bacillus cereus</i> degradation metabolites |
| title | Bioremediation of Neonicotinoid Pesticide, Imidacloprid, Mediated by <i>Bacillus cereus</i> |
| title_full | Bioremediation of Neonicotinoid Pesticide, Imidacloprid, Mediated by <i>Bacillus cereus</i> |
| title_fullStr | Bioremediation of Neonicotinoid Pesticide, Imidacloprid, Mediated by <i>Bacillus cereus</i> |
| title_full_unstemmed | Bioremediation of Neonicotinoid Pesticide, Imidacloprid, Mediated by <i>Bacillus cereus</i> |
| title_short | Bioremediation of Neonicotinoid Pesticide, Imidacloprid, Mediated by <i>Bacillus cereus</i> |
| title_sort | bioremediation of neonicotinoid pesticide imidacloprid mediated by i bacillus cereus i |
| topic | imidacloprid <i>Bacillus cereus</i> degradation metabolites |
| url | https://www.mdpi.com/2306-5354/10/8/951 |
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