Protective Effect and Potential Antioxidant Role of Kakadu Plum Extracts on Alcohol-Induced Oxidative Damage in HepG2 Cells
Serial alcohol consumption causes alcoholic liver disease (ALD), which can lead to fatty liver, hepatitis, and cirrhosis. <i>Terminalia ferdinandiana</i> (Kakadu plum) is an indigenous fruit of Australia, which is utilized as a functional food. It is a commercially important antioxidant...
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MDPI AG
2021-12-01
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| author | Reshmi Akter Gi-Young Kwak Jong Chan Ahn Ramya Mathiyalagan Zelika Mega Ramadhania Deok Chun Yang Se Chan Kang |
| author_facet | Reshmi Akter Gi-Young Kwak Jong Chan Ahn Ramya Mathiyalagan Zelika Mega Ramadhania Deok Chun Yang Se Chan Kang |
| author_sort | Reshmi Akter |
| collection | DOAJ |
| description | Serial alcohol consumption causes alcoholic liver disease (ALD), which can lead to fatty liver, hepatitis, and cirrhosis. <i>Terminalia ferdinandiana</i> (Kakadu plum) is an indigenous fruit of Australia, which is utilized as a functional food. It is a commercially important antioxidant as it contains a more eloquent level of ascorbic acid than other oranges. In this study, we analyzed the chemical constituents of vitamin C, gallic acid, ellagic acid, and daidzin via High-performance liquid chromatography (HPLC) in the Kakadu plum from two different regions including the Northern Territory (NT) and Western Australia (WA), and compared their biochemical properties. The vitamin C content was much higher (almost 70%) in Kakadu plum (KKD) from the NT than WA. Moreover, ROS generation was inhibited significantly in HepG2 (human hepatoma) cells with the KKD-NT extract treatment when compared to the KKD-WA extract treatment. The cytotoxicity produced by ethanol was significantly suppressed in response to the treatment with both of the samples. In addition, our samples (KKD-NT and KKD-WA) increased the activity of two key enzymes involving alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) that metabolize ethanol. These results show the biochemical confirmation of the mechanism by which KKD exhibits its biological functions including relief from alcohol hangovers as well as protection of the liver cells by the suppression of ROS production and toxic insults. |
| first_indexed | 2024-03-10T03:50:40Z |
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| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T03:50:40Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
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| series | Applied Sciences |
| spelling | doaj.art-cde7e7bbaed1472c941987dc35c63d952023-11-23T11:09:46ZengMDPI AGApplied Sciences2076-34172021-12-0112123610.3390/app12010236Protective Effect and Potential Antioxidant Role of Kakadu Plum Extracts on Alcohol-Induced Oxidative Damage in HepG2 CellsReshmi Akter0Gi-Young Kwak1Jong Chan Ahn2Ramya Mathiyalagan3Zelika Mega Ramadhania4Deok Chun Yang5Se Chan Kang6Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Yongin-si 17104, Gyeonggi-do, KoreaGraduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Yongin-si 17104, Gyeonggi-do, KoreaGraduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Yongin-si 17104, Gyeonggi-do, KoreaGraduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Yongin-si 17104, Gyeonggi-do, KoreaGraduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Yongin-si 17104, Gyeonggi-do, KoreaGraduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Yongin-si 17104, Gyeonggi-do, KoreaGraduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Yongin-si 17104, Gyeonggi-do, KoreaSerial alcohol consumption causes alcoholic liver disease (ALD), which can lead to fatty liver, hepatitis, and cirrhosis. <i>Terminalia ferdinandiana</i> (Kakadu plum) is an indigenous fruit of Australia, which is utilized as a functional food. It is a commercially important antioxidant as it contains a more eloquent level of ascorbic acid than other oranges. In this study, we analyzed the chemical constituents of vitamin C, gallic acid, ellagic acid, and daidzin via High-performance liquid chromatography (HPLC) in the Kakadu plum from two different regions including the Northern Territory (NT) and Western Australia (WA), and compared their biochemical properties. The vitamin C content was much higher (almost 70%) in Kakadu plum (KKD) from the NT than WA. Moreover, ROS generation was inhibited significantly in HepG2 (human hepatoma) cells with the KKD-NT extract treatment when compared to the KKD-WA extract treatment. The cytotoxicity produced by ethanol was significantly suppressed in response to the treatment with both of the samples. In addition, our samples (KKD-NT and KKD-WA) increased the activity of two key enzymes involving alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) that metabolize ethanol. These results show the biochemical confirmation of the mechanism by which KKD exhibits its biological functions including relief from alcohol hangovers as well as protection of the liver cells by the suppression of ROS production and toxic insults.https://www.mdpi.com/2076-3417/12/1/236Kakadu plumethanol metabolismlivertoxicityROS generation |
| spellingShingle | Reshmi Akter Gi-Young Kwak Jong Chan Ahn Ramya Mathiyalagan Zelika Mega Ramadhania Deok Chun Yang Se Chan Kang Protective Effect and Potential Antioxidant Role of Kakadu Plum Extracts on Alcohol-Induced Oxidative Damage in HepG2 Cells Applied Sciences Kakadu plum ethanol metabolism liver toxicity ROS generation |
| title | Protective Effect and Potential Antioxidant Role of Kakadu Plum Extracts on Alcohol-Induced Oxidative Damage in HepG2 Cells |
| title_full | Protective Effect and Potential Antioxidant Role of Kakadu Plum Extracts on Alcohol-Induced Oxidative Damage in HepG2 Cells |
| title_fullStr | Protective Effect and Potential Antioxidant Role of Kakadu Plum Extracts on Alcohol-Induced Oxidative Damage in HepG2 Cells |
| title_full_unstemmed | Protective Effect and Potential Antioxidant Role of Kakadu Plum Extracts on Alcohol-Induced Oxidative Damage in HepG2 Cells |
| title_short | Protective Effect and Potential Antioxidant Role of Kakadu Plum Extracts on Alcohol-Induced Oxidative Damage in HepG2 Cells |
| title_sort | protective effect and potential antioxidant role of kakadu plum extracts on alcohol induced oxidative damage in hepg2 cells |
| topic | Kakadu plum ethanol metabolism liver toxicity ROS generation |
| url | https://www.mdpi.com/2076-3417/12/1/236 |
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