Developing a Fast Ultra-High-Performance Liquid Chromatography–Tandem Mass Spectrometry Method for High-Throughput Surface Contamination Monitoring of 26 Antineoplastic Drugs
Growing attention on carcinogenicity and mutagenicity of antineoplastic drugs (ADs) from the International Agencies has led to the present strict safe handling and administration regulations. Accordingly, one of the most common ways to assess occupational exposure to these substances is to identify...
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MDPI AG
2021-09-01
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| Online Access: | https://www.mdpi.com/2297-8739/8/9/150 |
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| author | Stefano Dugheri Nicola Mucci Donato Squillaci Giorgio Marrubini Gianluca Bartolucci Camillo Melzi Elisabetta Bucaletti Giovanni Cappelli Lucia Trevisani Giulio Arcangeli |
| author_facet | Stefano Dugheri Nicola Mucci Donato Squillaci Giorgio Marrubini Gianluca Bartolucci Camillo Melzi Elisabetta Bucaletti Giovanni Cappelli Lucia Trevisani Giulio Arcangeli |
| author_sort | Stefano Dugheri |
| collection | DOAJ |
| description | Growing attention on carcinogenicity and mutagenicity of antineoplastic drugs (ADs) from the International Agencies has led to the present strict safe handling and administration regulations. Accordingly, one of the most common ways to assess occupational exposure to these substances is to identify and quantify possible surface contamination inside hospital preparation and administration units. Thus, it is essential to develop a fast and high-throughput monitoring method capable of identifying a significant number of ADs. The present study reports developing a UHPLC–MS/MS analysis to screen 26 ADs surface contamination through wipe test sampling. A Cortecs UPLC T3 50 × 2.1 mm (1.6 µm) column was selected to perform the analysis, using the evaluations of previous studies and the Product Quality Research Institute (PQRI) database. The design of experiments (DoE) methodological approach was used to optimize the chromatographic conditions concerning the best separation between all ADs. The limits of quantification for the analytes were between the pg/mL and ng/mL orders, and the turnaround time was limited to about 15 min. The obtained accuracy was mostly between 90% and 110% for all the analytes, while the precision was under 10% and a low matrix effect was observed for said analytes. Only vindesine and docetaxel presented lower performances. |
| first_indexed | 2024-03-10T07:13:51Z |
| format | Article |
| id | doaj.art-cde9bab59886407c8536291b630a3b07 |
| institution | Directory Open Access Journal |
| issn | 2297-8739 |
| language | English |
| last_indexed | 2024-03-10T07:13:51Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Separations |
| spelling | doaj.art-cde9bab59886407c8536291b630a3b072023-11-22T15:15:29ZengMDPI AGSeparations2297-87392021-09-018915010.3390/separations8090150Developing a Fast Ultra-High-Performance Liquid Chromatography–Tandem Mass Spectrometry Method for High-Throughput Surface Contamination Monitoring of 26 Antineoplastic DrugsStefano Dugheri0Nicola Mucci1Donato Squillaci2Giorgio Marrubini3Gianluca Bartolucci4Camillo Melzi5Elisabetta Bucaletti6Giovanni Cappelli7Lucia Trevisani8Giulio Arcangeli9Industrial Hygiene and Toxicology Laboratory, Occupational Medicine Unit, Careggi University Hospital, 50134 Florence, ItalyDepartment of Experimental and Clinical Medicine, University of Florence, 50134 Florence, ItalyDepartment of Experimental and Clinical Medicine, University of Florence, 50134 Florence, ItalyDepartment of Drug Sciences, University of Pavia, Via Taramelli 12, 27100 Pavia, ItalyDepartment of Neurosciences, Psychology, Drug Research and Child Health, University of Florence, 50019 Sesto Fiorentino, ItalyMetanalisi, Corso di Porta Venezia 40, 20121 Milano, ItalyDepartment of Experimental and Clinical Medicine, University of Florence, 50134 Florence, ItalyDepartment of Experimental and Clinical Medicine, University of Florence, 50134 Florence, ItalyDepartment of Experimental and Clinical Medicine, University of Florence, 50134 Florence, ItalyDepartment of Experimental and Clinical Medicine, University of Florence, 50134 Florence, ItalyGrowing attention on carcinogenicity and mutagenicity of antineoplastic drugs (ADs) from the International Agencies has led to the present strict safe handling and administration regulations. Accordingly, one of the most common ways to assess occupational exposure to these substances is to identify and quantify possible surface contamination inside hospital preparation and administration units. Thus, it is essential to develop a fast and high-throughput monitoring method capable of identifying a significant number of ADs. The present study reports developing a UHPLC–MS/MS analysis to screen 26 ADs surface contamination through wipe test sampling. A Cortecs UPLC T3 50 × 2.1 mm (1.6 µm) column was selected to perform the analysis, using the evaluations of previous studies and the Product Quality Research Institute (PQRI) database. The design of experiments (DoE) methodological approach was used to optimize the chromatographic conditions concerning the best separation between all ADs. The limits of quantification for the analytes were between the pg/mL and ng/mL orders, and the turnaround time was limited to about 15 min. The obtained accuracy was mostly between 90% and 110% for all the analytes, while the precision was under 10% and a low matrix effect was observed for said analytes. Only vindesine and docetaxel presented lower performances.https://www.mdpi.com/2297-8739/8/9/150antineoplastic drugswipe testultra-high-performance liquid chromatographytandem mass spectrometrydesign of experimentsPQRI |
| spellingShingle | Stefano Dugheri Nicola Mucci Donato Squillaci Giorgio Marrubini Gianluca Bartolucci Camillo Melzi Elisabetta Bucaletti Giovanni Cappelli Lucia Trevisani Giulio Arcangeli Developing a Fast Ultra-High-Performance Liquid Chromatography–Tandem Mass Spectrometry Method for High-Throughput Surface Contamination Monitoring of 26 Antineoplastic Drugs Separations antineoplastic drugs wipe test ultra-high-performance liquid chromatography tandem mass spectrometry design of experiments PQRI |
| title | Developing a Fast Ultra-High-Performance Liquid Chromatography–Tandem Mass Spectrometry Method for High-Throughput Surface Contamination Monitoring of 26 Antineoplastic Drugs |
| title_full | Developing a Fast Ultra-High-Performance Liquid Chromatography–Tandem Mass Spectrometry Method for High-Throughput Surface Contamination Monitoring of 26 Antineoplastic Drugs |
| title_fullStr | Developing a Fast Ultra-High-Performance Liquid Chromatography–Tandem Mass Spectrometry Method for High-Throughput Surface Contamination Monitoring of 26 Antineoplastic Drugs |
| title_full_unstemmed | Developing a Fast Ultra-High-Performance Liquid Chromatography–Tandem Mass Spectrometry Method for High-Throughput Surface Contamination Monitoring of 26 Antineoplastic Drugs |
| title_short | Developing a Fast Ultra-High-Performance Liquid Chromatography–Tandem Mass Spectrometry Method for High-Throughput Surface Contamination Monitoring of 26 Antineoplastic Drugs |
| title_sort | developing a fast ultra high performance liquid chromatography tandem mass spectrometry method for high throughput surface contamination monitoring of 26 antineoplastic drugs |
| topic | antineoplastic drugs wipe test ultra-high-performance liquid chromatography tandem mass spectrometry design of experiments PQRI |
| url | https://www.mdpi.com/2297-8739/8/9/150 |
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