Anti-Inflammatory Properties of <i>Bellevalia saviczii</i> Root Extract and Its Isolated Homoisoflavonoid (<i>Dracol</i>) Are Mediated by Modification on Calcium Signaling
<i>Bellevalia saviczii</i> is a medicinal plant used as anti-rheumatic and anti-inflammatory herbal remedy in Iraqi-Kurdistan. The aim of this study was to evaluate the anti-inflammatory activity of its extract and the isolated homoisoflavonoid (<i>Dracol</i>) by studying the...
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MDPI AG
2019-09-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/24/18/3376 |
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| author | Monica Savio Mohammed Farhad Ibrahim Chiara Scarlata Matteo Orgiu Giuseppe Accardo Abdullah Shakur Sardar Francesco Moccia Lucia Anna Stivala Gloria Brusotti |
| author_facet | Monica Savio Mohammed Farhad Ibrahim Chiara Scarlata Matteo Orgiu Giuseppe Accardo Abdullah Shakur Sardar Francesco Moccia Lucia Anna Stivala Gloria Brusotti |
| author_sort | Monica Savio |
| collection | DOAJ |
| description | <i>Bellevalia saviczii</i> is a medicinal plant used as anti-rheumatic and anti-inflammatory herbal remedy in Iraqi-Kurdistan. The aim of this study was to evaluate the anti-inflammatory activity of its extract and the isolated homoisoflavonoid (<i>Dracol</i>) by studying the Ca<sup>2+</sup>-dependent NF-kB pathway. Nuclear translocation of p65 NF-kB subunit, as parameter of NF-kB activation, was visualized in human leukemic monocytes by immunofluorescence and Western blot analyses, after cell treatment with <i>B. saviczii</i> root extract or <i>Dracol</i> followed by Lipopolysaccharide stimulation. In parallel, Ca<sup>2+</sup> signals responsible for NF-kB activation and levels of inflammatory cytokines were investigated. LPS-induced p65 translocation was evident in monocytes and both treatments, in particular that with <i>Dracol</i>, were able to counteract this activation. Intracellular Ca<sup>2+</sup> oscillations were halted and the cytokine release reduced. These results confirm the traditional anti-inflammatory efficacy of <i>B. saviczii</i> and identify one of the molecules in the extract which appears to be responsible of this action. |
| first_indexed | 2024-12-11T22:54:50Z |
| format | Article |
| id | doaj.art-cdea94ef2ad74bbaad134442f1e61926 |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-11T22:54:50Z |
| publishDate | 2019-09-01 |
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| record_format | Article |
| series | Molecules |
| spelling | doaj.art-cdea94ef2ad74bbaad134442f1e619262022-12-22T00:47:17ZengMDPI AGMolecules1420-30492019-09-012418337610.3390/molecules24183376molecules24183376Anti-Inflammatory Properties of <i>Bellevalia saviczii</i> Root Extract and Its Isolated Homoisoflavonoid (<i>Dracol</i>) Are Mediated by Modification on Calcium SignalingMonica Savio0Mohammed Farhad Ibrahim1Chiara Scarlata2Matteo Orgiu3Giuseppe Accardo4Abdullah Shakur Sardar5Francesco Moccia6Lucia Anna Stivala7Gloria Brusotti8Department of Molecular Medicine, Immunology and General Pathology Unit, via Ferrata 9, University of Pavia, 27100 Pavia, ItalyDepartment of Drug Sciences, viale Taramelli 12, University of Pavia, 27100 Pavia, ItalyDepartment of Molecular Medicine, Immunology and General Pathology Unit, via Ferrata 9, University of Pavia, 27100 Pavia, ItalyDepartment of Biology and Biotechnology “L. Spallanzani” via Forlanini 6, University of Pavia, 27100 Pavia, ItalyDepartment of Molecular Medicine, Immunology and General Pathology Unit, via Ferrata 9, University of Pavia, 27100 Pavia, ItalyDepartment of Biology, College of Education, University of Salahaddin-Erbil, Erbil 44001, IraqDepartment of Biology and Biotechnology “L. Spallanzani” via Forlanini 6, University of Pavia, 27100 Pavia, ItalyDepartment of Molecular Medicine, Immunology and General Pathology Unit, via Ferrata 9, University of Pavia, 27100 Pavia, ItalyDepartment of Drug Sciences, viale Taramelli 12, University of Pavia, 27100 Pavia, Italy<i>Bellevalia saviczii</i> is a medicinal plant used as anti-rheumatic and anti-inflammatory herbal remedy in Iraqi-Kurdistan. The aim of this study was to evaluate the anti-inflammatory activity of its extract and the isolated homoisoflavonoid (<i>Dracol</i>) by studying the Ca<sup>2+</sup>-dependent NF-kB pathway. Nuclear translocation of p65 NF-kB subunit, as parameter of NF-kB activation, was visualized in human leukemic monocytes by immunofluorescence and Western blot analyses, after cell treatment with <i>B. saviczii</i> root extract or <i>Dracol</i> followed by Lipopolysaccharide stimulation. In parallel, Ca<sup>2+</sup> signals responsible for NF-kB activation and levels of inflammatory cytokines were investigated. LPS-induced p65 translocation was evident in monocytes and both treatments, in particular that with <i>Dracol</i>, were able to counteract this activation. Intracellular Ca<sup>2+</sup> oscillations were halted and the cytokine release reduced. These results confirm the traditional anti-inflammatory efficacy of <i>B. saviczii</i> and identify one of the molecules in the extract which appears to be responsible of this action.https://www.mdpi.com/1420-3049/24/18/3376<i>Bellevalia saviczii</i>homoisoflavoinoidsNF-kBCa<sup>2+</sup> signaling |
| spellingShingle | Monica Savio Mohammed Farhad Ibrahim Chiara Scarlata Matteo Orgiu Giuseppe Accardo Abdullah Shakur Sardar Francesco Moccia Lucia Anna Stivala Gloria Brusotti Anti-Inflammatory Properties of <i>Bellevalia saviczii</i> Root Extract and Its Isolated Homoisoflavonoid (<i>Dracol</i>) Are Mediated by Modification on Calcium Signaling Molecules <i>Bellevalia saviczii</i> homoisoflavoinoids NF-kB Ca<sup>2+</sup> signaling |
| title | Anti-Inflammatory Properties of <i>Bellevalia saviczii</i> Root Extract and Its Isolated Homoisoflavonoid (<i>Dracol</i>) Are Mediated by Modification on Calcium Signaling |
| title_full | Anti-Inflammatory Properties of <i>Bellevalia saviczii</i> Root Extract and Its Isolated Homoisoflavonoid (<i>Dracol</i>) Are Mediated by Modification on Calcium Signaling |
| title_fullStr | Anti-Inflammatory Properties of <i>Bellevalia saviczii</i> Root Extract and Its Isolated Homoisoflavonoid (<i>Dracol</i>) Are Mediated by Modification on Calcium Signaling |
| title_full_unstemmed | Anti-Inflammatory Properties of <i>Bellevalia saviczii</i> Root Extract and Its Isolated Homoisoflavonoid (<i>Dracol</i>) Are Mediated by Modification on Calcium Signaling |
| title_short | Anti-Inflammatory Properties of <i>Bellevalia saviczii</i> Root Extract and Its Isolated Homoisoflavonoid (<i>Dracol</i>) Are Mediated by Modification on Calcium Signaling |
| title_sort | anti inflammatory properties of i bellevalia saviczii i root extract and its isolated homoisoflavonoid i dracol i are mediated by modification on calcium signaling |
| topic | <i>Bellevalia saviczii</i> homoisoflavoinoids NF-kB Ca<sup>2+</sup> signaling |
| url | https://www.mdpi.com/1420-3049/24/18/3376 |
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