Highly purified human peripheral blood monocytes produce IL-6 but not TNFα in response to angiotensin II
Hypothesis : Monocytes produce pro-inflammatory cytokines in response to Angiotensin II (AngII). Introduction : AngII has been suggested by many to be pro-inflammatory and likely to contribute to the migration of leukocytes in patients with cardiovascular conditions. Materials and methods : Monocyte...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publications
2011-09-01
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| Series: | Journal of the Renin-Angiotensin-Aldosterone System |
| Online Access: | https://doi.org/10.1177/1470320310391332 |
| _version_ | 1797288997815320576 |
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| author | Laura Gelinas Alec Falkenham Adam Oxner Mryanda Sopel Jean-Francois Légaré |
| author_facet | Laura Gelinas Alec Falkenham Adam Oxner Mryanda Sopel Jean-Francois Légaré |
| author_sort | Laura Gelinas |
| collection | DOAJ |
| description | Hypothesis : Monocytes produce pro-inflammatory cytokines in response to Angiotensin II (AngII). Introduction : AngII has been suggested by many to be pro-inflammatory and likely to contribute to the migration of leukocytes in patients with cardiovascular conditions. Materials and methods : Monocytes were purified from peripheral blood mononuclear cells (PBMCs) by negative selection using antibodies conjugated to magnetic beads. Detection of CD14 + and AT 1 R expression was achieved by double-labeling flow cytometry. Highly purified monocytes were then stimulated with AngII (6 and 24 h) to assess IL-6 and TNF-α transcript levels by qRT-PCR and protein secretion by ELISA. Results : Monocytes comprised 9.7 ± 2.0% of the PBMCs. Monocyte isolation by negative selection yielded a purity of up to 99.8%. We demonstrated AT 1 R expression on 9.5 ± 0.3% of highly purifed CD14 + /CD16 - monocytes. Stimulation of highly purified monocytes with AngII resulted in increased transcript levels of IL-6 at 6 h but not at 24 h, and increased secretion of IL-6 in a dose-dependent manner compared with controls ( p <0.01). Conversely, there was no increase in TNF-α mRNA transcripts or protein secretion. Conclusions : We provide evidence that a CD14 + /CD16 - subset of highly purified human monocytes express AT 1 R and respond to AngII exposure in vitro by producing IL-6 but not TNF-α. |
| first_indexed | 2024-03-07T18:57:43Z |
| format | Article |
| id | doaj.art-cdee8fc048494ce7bfaf56533a5cba8b |
| institution | Directory Open Access Journal |
| issn | 1470-3203 |
| language | English |
| last_indexed | 2024-03-07T18:57:43Z |
| publishDate | 2011-09-01 |
| publisher | SAGE Publications |
| record_format | Article |
| series | Journal of the Renin-Angiotensin-Aldosterone System |
| spelling | doaj.art-cdee8fc048494ce7bfaf56533a5cba8b2024-03-02T00:08:16ZengSAGE PublicationsJournal of the Renin-Angiotensin-Aldosterone System1470-32032011-09-011210.1177/1470320310391332Highly purified human peripheral blood monocytes produce IL-6 but not TNFα in response to angiotensin IILaura GelinasAlec FalkenhamAdam OxnerMryanda SopelJean-Francois LégaréHypothesis : Monocytes produce pro-inflammatory cytokines in response to Angiotensin II (AngII). Introduction : AngII has been suggested by many to be pro-inflammatory and likely to contribute to the migration of leukocytes in patients with cardiovascular conditions. Materials and methods : Monocytes were purified from peripheral blood mononuclear cells (PBMCs) by negative selection using antibodies conjugated to magnetic beads. Detection of CD14 + and AT 1 R expression was achieved by double-labeling flow cytometry. Highly purified monocytes were then stimulated with AngII (6 and 24 h) to assess IL-6 and TNF-α transcript levels by qRT-PCR and protein secretion by ELISA. Results : Monocytes comprised 9.7 ± 2.0% of the PBMCs. Monocyte isolation by negative selection yielded a purity of up to 99.8%. We demonstrated AT 1 R expression on 9.5 ± 0.3% of highly purifed CD14 + /CD16 - monocytes. Stimulation of highly purified monocytes with AngII resulted in increased transcript levels of IL-6 at 6 h but not at 24 h, and increased secretion of IL-6 in a dose-dependent manner compared with controls ( p <0.01). Conversely, there was no increase in TNF-α mRNA transcripts or protein secretion. Conclusions : We provide evidence that a CD14 + /CD16 - subset of highly purified human monocytes express AT 1 R and respond to AngII exposure in vitro by producing IL-6 but not TNF-α.https://doi.org/10.1177/1470320310391332 |
| spellingShingle | Laura Gelinas Alec Falkenham Adam Oxner Mryanda Sopel Jean-Francois Légaré Highly purified human peripheral blood monocytes produce IL-6 but not TNFα in response to angiotensin II Journal of the Renin-Angiotensin-Aldosterone System |
| title | Highly purified human peripheral blood monocytes produce IL-6 but not TNFα in response to angiotensin II |
| title_full | Highly purified human peripheral blood monocytes produce IL-6 but not TNFα in response to angiotensin II |
| title_fullStr | Highly purified human peripheral blood monocytes produce IL-6 but not TNFα in response to angiotensin II |
| title_full_unstemmed | Highly purified human peripheral blood monocytes produce IL-6 but not TNFα in response to angiotensin II |
| title_short | Highly purified human peripheral blood monocytes produce IL-6 but not TNFα in response to angiotensin II |
| title_sort | highly purified human peripheral blood monocytes produce il 6 but not tnfα in response to angiotensin ii |
| url | https://doi.org/10.1177/1470320310391332 |
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