Isolation of <it>Pediococcus acidilactici</it> Kp10 with ability to secrete bacteriocin-like inhibitory substance from milk products for applications in food industry
<p>Abstract</p> <p>Background</p> <p>Lactic acid bacteria (LAB) can be isolated from traditional milk products. LAB that secrete substances that inhibit pathogenic bacteria and are resistant to acid, bile, and pepsin but not vancomycin may have potential in food applica...
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| Format: | Article |
| Language: | English |
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BMC
2012-11-01
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| Series: | BMC Microbiology |
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| Online Access: | http://www.biomedcentral.com/1471-2180/12/260 |
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| author | Abbasiliasi Sahar Tan Joo Shun Ibrahim Tengku Azmi Tengku Ramanan Ramakrishnan Nagasundara Vakhshiteh Faezeh Mustafa Shuhaimi Ling Tau Chuan Rahim Raha Abdul Ariff Arbakariya B |
| author_facet | Abbasiliasi Sahar Tan Joo Shun Ibrahim Tengku Azmi Tengku Ramanan Ramakrishnan Nagasundara Vakhshiteh Faezeh Mustafa Shuhaimi Ling Tau Chuan Rahim Raha Abdul Ariff Arbakariya B |
| author_sort | Abbasiliasi Sahar |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Lactic acid bacteria (LAB) can be isolated from traditional milk products. LAB that secrete substances that inhibit pathogenic bacteria and are resistant to acid, bile, and pepsin but not vancomycin may have potential in food applications.</p> <p>Results</p> <p>LAB isolated from a range of traditional fermented products were screened for the production of bacteriocin-like inhibitory substances. A total of 222 LAB strains were isolated from fermented milk products in the form of fresh curds, dried curds, and ghara (a traditional flavor enhancer prepared from whey), and fermented cocoa bean. Eleven LAB isolates that produced antimicrobial substances were identified as <it>Lactococcus lactis</it>, <it>Lactobacillus plantarum</it>, and <it>Pediococcus acidilactici</it> strains by biochemical methods and 16S rDNA gene sequencing. Of these, the cell-free supernatant of Kp10 (<it>P. acidilactici</it>) most strongly inhibited <it>Listeria monocytogenes</it>. Further analysis identified the antimicrobial substance produced by Kp10 as proteinaceous in nature and active over a wide pH range. Kp10 (<it>P. acidilactici</it>) was found to be catalase-negative, able to produce β-galactosidase, resistant to bile salts (0.3%) and acidic conditions (pH 3), and susceptible to most antibiotics.</p> <p>Conclusion</p> <p>Traditionally prepared fermented milk products are good sources of LAB with characteristics suitable for industrial applications. The isolate Kp10 (<it>P. acidilactici</it>) shows potential for the production of probiotic and functional foods.</p> |
| first_indexed | 2024-12-10T16:01:05Z |
| format | Article |
| id | doaj.art-cdf12c086c9e451ca0ca6e0744f4da33 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-10T16:01:05Z |
| publishDate | 2012-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-cdf12c086c9e451ca0ca6e0744f4da332022-12-22T01:42:26ZengBMCBMC Microbiology1471-21802012-11-0112126010.1186/1471-2180-12-260Isolation of <it>Pediococcus acidilactici</it> Kp10 with ability to secrete bacteriocin-like inhibitory substance from milk products for applications in food industryAbbasiliasi SaharTan Joo ShunIbrahim Tengku Azmi TengkuRamanan Ramakrishnan NagasundaraVakhshiteh FaezehMustafa ShuhaimiLing Tau ChuanRahim Raha AbdulAriff Arbakariya B<p>Abstract</p> <p>Background</p> <p>Lactic acid bacteria (LAB) can be isolated from traditional milk products. LAB that secrete substances that inhibit pathogenic bacteria and are resistant to acid, bile, and pepsin but not vancomycin may have potential in food applications.</p> <p>Results</p> <p>LAB isolated from a range of traditional fermented products were screened for the production of bacteriocin-like inhibitory substances. A total of 222 LAB strains were isolated from fermented milk products in the form of fresh curds, dried curds, and ghara (a traditional flavor enhancer prepared from whey), and fermented cocoa bean. Eleven LAB isolates that produced antimicrobial substances were identified as <it>Lactococcus lactis</it>, <it>Lactobacillus plantarum</it>, and <it>Pediococcus acidilactici</it> strains by biochemical methods and 16S rDNA gene sequencing. Of these, the cell-free supernatant of Kp10 (<it>P. acidilactici</it>) most strongly inhibited <it>Listeria monocytogenes</it>. Further analysis identified the antimicrobial substance produced by Kp10 as proteinaceous in nature and active over a wide pH range. Kp10 (<it>P. acidilactici</it>) was found to be catalase-negative, able to produce β-galactosidase, resistant to bile salts (0.3%) and acidic conditions (pH 3), and susceptible to most antibiotics.</p> <p>Conclusion</p> <p>Traditionally prepared fermented milk products are good sources of LAB with characteristics suitable for industrial applications. The isolate Kp10 (<it>P. acidilactici</it>) shows potential for the production of probiotic and functional foods.</p>http://www.biomedcentral.com/1471-2180/12/260Lactic acid bacteria<it>Pediococcus acidilactici</it>Bacteriocin-like inhibitory substance<it>Listeria monocytogenes</it>FermentationIdentification |
| spellingShingle | Abbasiliasi Sahar Tan Joo Shun Ibrahim Tengku Azmi Tengku Ramanan Ramakrishnan Nagasundara Vakhshiteh Faezeh Mustafa Shuhaimi Ling Tau Chuan Rahim Raha Abdul Ariff Arbakariya B Isolation of <it>Pediococcus acidilactici</it> Kp10 with ability to secrete bacteriocin-like inhibitory substance from milk products for applications in food industry BMC Microbiology Lactic acid bacteria <it>Pediococcus acidilactici</it> Bacteriocin-like inhibitory substance <it>Listeria monocytogenes</it> Fermentation Identification |
| title | Isolation of <it>Pediococcus acidilactici</it> Kp10 with ability to secrete bacteriocin-like inhibitory substance from milk products for applications in food industry |
| title_full | Isolation of <it>Pediococcus acidilactici</it> Kp10 with ability to secrete bacteriocin-like inhibitory substance from milk products for applications in food industry |
| title_fullStr | Isolation of <it>Pediococcus acidilactici</it> Kp10 with ability to secrete bacteriocin-like inhibitory substance from milk products for applications in food industry |
| title_full_unstemmed | Isolation of <it>Pediococcus acidilactici</it> Kp10 with ability to secrete bacteriocin-like inhibitory substance from milk products for applications in food industry |
| title_short | Isolation of <it>Pediococcus acidilactici</it> Kp10 with ability to secrete bacteriocin-like inhibitory substance from milk products for applications in food industry |
| title_sort | isolation of it pediococcus acidilactici it kp10 with ability to secrete bacteriocin like inhibitory substance from milk products for applications in food industry |
| topic | Lactic acid bacteria <it>Pediococcus acidilactici</it> Bacteriocin-like inhibitory substance <it>Listeria monocytogenes</it> Fermentation Identification |
| url | http://www.biomedcentral.com/1471-2180/12/260 |
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