Fecal microbiota in patients with a stoma decreases anaerobic bacteria and alters taxonomic and functional diversities
Colorectal cancer (CRC) is one of the most common malignant diseases. Generally, stoma construction is performed following surgery for the resection of the primary tumor in patients with CRC. The association of CRC with the gut microbiota has been widely reported, and the gut microbiota is known to...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2022.925444/full |
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| author | Shunsuke A. Sakai Shunsuke A. Sakai Masato Aoshima Masato Aoshima Kentaro Sawada Satoshi Horasawa Ayumu Yoshikawa Takao Fujisawa Shigenori Kadowaki Tadamichi Denda Nobuhisa Matsuhashi Hisateru Yasui Masahiro Goto Kentaro Yamazaki Yoshito Komatsu Ryota Nakanishi Yoshiaki Nakamura Yoshiaki Nakamura Hideaki Bando Hideaki Bando Yamato Hamaya Yamato Hamaya Shun-Ichiro Kageyama Takayuki Yoshino Katsuya Tsuchihara Katsuya Tsuchihara Riu Yamashita Riu Yamashita |
| author_facet | Shunsuke A. Sakai Shunsuke A. Sakai Masato Aoshima Masato Aoshima Kentaro Sawada Satoshi Horasawa Ayumu Yoshikawa Takao Fujisawa Shigenori Kadowaki Tadamichi Denda Nobuhisa Matsuhashi Hisateru Yasui Masahiro Goto Kentaro Yamazaki Yoshito Komatsu Ryota Nakanishi Yoshiaki Nakamura Yoshiaki Nakamura Hideaki Bando Hideaki Bando Yamato Hamaya Yamato Hamaya Shun-Ichiro Kageyama Takayuki Yoshino Katsuya Tsuchihara Katsuya Tsuchihara Riu Yamashita Riu Yamashita |
| author_sort | Shunsuke A. Sakai |
| collection | DOAJ |
| description | Colorectal cancer (CRC) is one of the most common malignant diseases. Generally, stoma construction is performed following surgery for the resection of the primary tumor in patients with CRC. The association of CRC with the gut microbiota has been widely reported, and the gut microbiota is known to play an important role in the carcinogenesis, progression, and treatment of CRC. In this study, we compared the microbiota of patients with CRC between with and without a stoma using fecal metagenomic sequencing data from SCRUM-Japan MONSTAR-SCREEN, a joint industry-academia cancer research project in Japan. We found that the composition of anaerobes was reduced in patients with a stoma. In particular, the abundance of Alistipes, Akkermansia, Intestinimonas, and methane-producing archaea decreased. We also compared gene function (e.g., KEGG Orthology and KEGG pathway) and found that gene function for methane and short-chain fatty acids (SCFAs) production was underrepresented in patients with a stoma. Furthermore, a stoma decreased Shannon diversity based on taxonomic composition but increased that of the KEGG pathway. These results suggest that the feces of patients with a stoma have a reduced abundance of favorable microbes for cancer immunotherapy. In conclusion, we showed that a stoma alters the taxonomic and functional profiles in feces and may be a confounding factor in fecal microbiota analysis. |
| first_indexed | 2024-04-12T21:47:52Z |
| format | Article |
| id | doaj.art-cdf35b72502c4aafb18359cbff9d7ada |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-04-12T21:47:52Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-cdf35b72502c4aafb18359cbff9d7ada2022-12-22T03:15:34ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882022-09-011210.3389/fcimb.2022.925444925444Fecal microbiota in patients with a stoma decreases anaerobic bacteria and alters taxonomic and functional diversitiesShunsuke A. Sakai0Shunsuke A. Sakai1Masato Aoshima2Masato Aoshima3Kentaro Sawada4Satoshi Horasawa5Ayumu Yoshikawa6Takao Fujisawa7Shigenori Kadowaki8Tadamichi Denda9Nobuhisa Matsuhashi10Hisateru Yasui11Masahiro Goto12Kentaro Yamazaki13Yoshito Komatsu14Ryota Nakanishi15Yoshiaki Nakamura16Yoshiaki Nakamura17Hideaki Bando18Hideaki Bando19Yamato Hamaya20Yamato Hamaya21Shun-Ichiro Kageyama22Takayuki Yoshino23Katsuya Tsuchihara24Katsuya Tsuchihara25Riu Yamashita26Riu Yamashita27Graduate School of Frontier Science, Department of Integrated Biosciences, University of Tokyo, Kashiwa, JapanDivision of Translational Informatics, Exploratory Oncology Research and Clinical Trial Center, National Cancer Center, Kashiwa, JapanGraduate School of Frontier Science, Department of Integrated Biosciences, University of Tokyo, Kashiwa, JapanDivision of Translational Informatics, Exploratory Oncology Research and Clinical Trial Center, National Cancer Center, Kashiwa, JapanDepartment of Medical Oncology, Kushiro Rosai Hospital, Kushiro, JapanTranslational Research Support Section, National Cancer Center Hospital East, National Cancer Center Hospital East, Kashiwa, JapanDepartment of Gastroenterology and Gastrointestinal Oncology, National Cancer Center Hospital East, Kashiwa, JapanDepartment Head and Neck Medical Oncology, National Cancer Center Hospital East, Kashiwa, JapanDepartment of Clinical Oncology, Aichi Cancer Center Hospital, Nagoya, JapanDivisioin of Gastroenterology, Chiba Cancer Center, Chiba, JapanDepartment of Gastroenterological surgery Pediatric surgery, Gifu University Hospital, Gifu, Japan0Department of Medical Oncology, Kobe City Medical Center General Hospital, Kobe, Japan1Cancer Chemotherapy Center, Osaka Medical and Pharmaceutical University Hospital, Takatsuki, Japan2Division of Gastrointestinal Oncology, Shizuoka Cancer Center, Shizuoka, Japan3Department of Cancer Center, Hokkaido University Hospital, Hokkaido, Japan4Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, JapanTranslational Research Support Section, National Cancer Center Hospital East, National Cancer Center Hospital East, Kashiwa, JapanDepartment of Gastroenterology and Gastrointestinal Oncology, National Cancer Center Hospital East, Kashiwa, JapanTranslational Research Support Section, National Cancer Center Hospital East, National Cancer Center Hospital East, Kashiwa, JapanDepartment of Gastroenterology and Gastrointestinal Oncology, National Cancer Center Hospital East, Kashiwa, JapanGraduate School of Frontier Science, Department of Integrated Biosciences, University of Tokyo, Kashiwa, JapanDivision of Translational Informatics, Exploratory Oncology Research and Clinical Trial Center, National Cancer Center, Kashiwa, Japan5Department of Radiation Oncology and Particle Therapy, National Cancer Center Hospital East, Kashiwa, JapanDepartment of Gastroenterology and Gastrointestinal Oncology, National Cancer Center Hospital East, Kashiwa, JapanGraduate School of Frontier Science, Department of Integrated Biosciences, University of Tokyo, Kashiwa, JapanDivision of Translational Informatics, Exploratory Oncology Research and Clinical Trial Center, National Cancer Center, Kashiwa, JapanDivision of Translational Informatics, Exploratory Oncology Research and Clinical Trial Center, National Cancer Center, Kashiwa, Japan6Department of Computational Biology and Medical Sciences, Graduate School of Frontier Science, University of Tokyo, Kashiwa, JapanColorectal cancer (CRC) is one of the most common malignant diseases. Generally, stoma construction is performed following surgery for the resection of the primary tumor in patients with CRC. The association of CRC with the gut microbiota has been widely reported, and the gut microbiota is known to play an important role in the carcinogenesis, progression, and treatment of CRC. In this study, we compared the microbiota of patients with CRC between with and without a stoma using fecal metagenomic sequencing data from SCRUM-Japan MONSTAR-SCREEN, a joint industry-academia cancer research project in Japan. We found that the composition of anaerobes was reduced in patients with a stoma. In particular, the abundance of Alistipes, Akkermansia, Intestinimonas, and methane-producing archaea decreased. We also compared gene function (e.g., KEGG Orthology and KEGG pathway) and found that gene function for methane and short-chain fatty acids (SCFAs) production was underrepresented in patients with a stoma. Furthermore, a stoma decreased Shannon diversity based on taxonomic composition but increased that of the KEGG pathway. These results suggest that the feces of patients with a stoma have a reduced abundance of favorable microbes for cancer immunotherapy. In conclusion, we showed that a stoma alters the taxonomic and functional profiles in feces and may be a confounding factor in fecal microbiota analysis.https://www.frontiersin.org/articles/10.3389/fcimb.2022.925444/fullgut microbiotaanaerobestomacolostomycolorectal cancer16S rRNA gene |
| spellingShingle | Shunsuke A. Sakai Shunsuke A. Sakai Masato Aoshima Masato Aoshima Kentaro Sawada Satoshi Horasawa Ayumu Yoshikawa Takao Fujisawa Shigenori Kadowaki Tadamichi Denda Nobuhisa Matsuhashi Hisateru Yasui Masahiro Goto Kentaro Yamazaki Yoshito Komatsu Ryota Nakanishi Yoshiaki Nakamura Yoshiaki Nakamura Hideaki Bando Hideaki Bando Yamato Hamaya Yamato Hamaya Shun-Ichiro Kageyama Takayuki Yoshino Katsuya Tsuchihara Katsuya Tsuchihara Riu Yamashita Riu Yamashita Fecal microbiota in patients with a stoma decreases anaerobic bacteria and alters taxonomic and functional diversities Frontiers in Cellular and Infection Microbiology gut microbiota anaerobe stoma colostomy colorectal cancer 16S rRNA gene |
| title | Fecal microbiota in patients with a stoma decreases anaerobic bacteria and alters taxonomic and functional diversities |
| title_full | Fecal microbiota in patients with a stoma decreases anaerobic bacteria and alters taxonomic and functional diversities |
| title_fullStr | Fecal microbiota in patients with a stoma decreases anaerobic bacteria and alters taxonomic and functional diversities |
| title_full_unstemmed | Fecal microbiota in patients with a stoma decreases anaerobic bacteria and alters taxonomic and functional diversities |
| title_short | Fecal microbiota in patients with a stoma decreases anaerobic bacteria and alters taxonomic and functional diversities |
| title_sort | fecal microbiota in patients with a stoma decreases anaerobic bacteria and alters taxonomic and functional diversities |
| topic | gut microbiota anaerobe stoma colostomy colorectal cancer 16S rRNA gene |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2022.925444/full |
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