The Plasmodium falciparum translationally controlled tumor protein (TCTP) is incorporated more efficiently into B cells than its human homologue.
Plasmodium falciparum secretes a homologue of the translationally controlled tumor protein (TCTP) into serum of infected individuals, although its role in pathogenesis or virulence is unknown. To determine the effect of P. falciparum TCTP on B cells as compared to human TCTP, fluorescently labeled p...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3894975?pdf=render |
| _version_ | 1819044680263794688 |
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| author | Berenice Calderón-Pérez Beatriz Xoconostle-Cázares Rosalía Lira-Carmona Rosaura Hernández-Rivas Jaime Ortega-López Roberto Ruiz-Medrano |
| author_facet | Berenice Calderón-Pérez Beatriz Xoconostle-Cázares Rosalía Lira-Carmona Rosaura Hernández-Rivas Jaime Ortega-López Roberto Ruiz-Medrano |
| author_sort | Berenice Calderón-Pérez |
| collection | DOAJ |
| description | Plasmodium falciparum secretes a homologue of the translationally controlled tumor protein (TCTP) into serum of infected individuals, although its role in pathogenesis or virulence is unknown. To determine the effect of P. falciparum TCTP on B cells as compared to human TCTP, fluorescently labeled proteins were incubated on primary cultures of mouse splenic B cells and analyzed by flow cytometry and confocal microscopy. Our results indicate that both recombinant proteins are incorporated into B cells, but differ significantly in their rate and percentage of incorporation, being significantly higher for P. falciparum TCTP. Furthermore, P. falciparum TCTP showed a lower B cell proliferative effect than human TCTP, suggesting a mechanism through which the former could interfere in the host's immune response. |
| first_indexed | 2024-12-21T10:16:31Z |
| format | Article |
| id | doaj.art-cdf67c78b71b4828a2aea2f3b784420d |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T10:16:31Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-cdf67c78b71b4828a2aea2f3b784420d2022-12-21T19:07:35ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0191e8551410.1371/journal.pone.0085514The Plasmodium falciparum translationally controlled tumor protein (TCTP) is incorporated more efficiently into B cells than its human homologue.Berenice Calderón-PérezBeatriz Xoconostle-CázaresRosalía Lira-CarmonaRosaura Hernández-RivasJaime Ortega-LópezRoberto Ruiz-MedranoPlasmodium falciparum secretes a homologue of the translationally controlled tumor protein (TCTP) into serum of infected individuals, although its role in pathogenesis or virulence is unknown. To determine the effect of P. falciparum TCTP on B cells as compared to human TCTP, fluorescently labeled proteins were incubated on primary cultures of mouse splenic B cells and analyzed by flow cytometry and confocal microscopy. Our results indicate that both recombinant proteins are incorporated into B cells, but differ significantly in their rate and percentage of incorporation, being significantly higher for P. falciparum TCTP. Furthermore, P. falciparum TCTP showed a lower B cell proliferative effect than human TCTP, suggesting a mechanism through which the former could interfere in the host's immune response.http://europepmc.org/articles/PMC3894975?pdf=render |
| spellingShingle | Berenice Calderón-Pérez Beatriz Xoconostle-Cázares Rosalía Lira-Carmona Rosaura Hernández-Rivas Jaime Ortega-López Roberto Ruiz-Medrano The Plasmodium falciparum translationally controlled tumor protein (TCTP) is incorporated more efficiently into B cells than its human homologue. PLoS ONE |
| title | The Plasmodium falciparum translationally controlled tumor protein (TCTP) is incorporated more efficiently into B cells than its human homologue. |
| title_full | The Plasmodium falciparum translationally controlled tumor protein (TCTP) is incorporated more efficiently into B cells than its human homologue. |
| title_fullStr | The Plasmodium falciparum translationally controlled tumor protein (TCTP) is incorporated more efficiently into B cells than its human homologue. |
| title_full_unstemmed | The Plasmodium falciparum translationally controlled tumor protein (TCTP) is incorporated more efficiently into B cells than its human homologue. |
| title_short | The Plasmodium falciparum translationally controlled tumor protein (TCTP) is incorporated more efficiently into B cells than its human homologue. |
| title_sort | plasmodium falciparum translationally controlled tumor protein tctp is incorporated more efficiently into b cells than its human homologue |
| url | http://europepmc.org/articles/PMC3894975?pdf=render |
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