Fermented Rhus Verniciflua Stokes Extract Alleviates Nonalcoholic Fatty Liver through the AMPK/SREBP1/PCSK9 Pathway in HFD-Induced Nonalcoholic Fatty Liver Animal Model
Background: We have previously reported the anti-hepatic lipogenic effect of fermented <i>Rhus verniciflua</i> stokes extract (FRVE) in an oleic-acid-treated HepG2 cell model. Methods: Herein, we advanced our understanding and evaluated the impact of FRVE in HFD-fed C57BL/6 mice using an...
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MDPI AG
2020-09-01
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| Online Access: | https://www.mdpi.com/2076-3417/10/19/6833 |
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| author | Seon-Ok Lee Yinzhu Xu Hengmin Han Seok-Tae Jeong You-Kyung Lee Jean Kyung Paik Jin-Sol Cha Hyo-Jeong Lee |
| author_facet | Seon-Ok Lee Yinzhu Xu Hengmin Han Seok-Tae Jeong You-Kyung Lee Jean Kyung Paik Jin-Sol Cha Hyo-Jeong Lee |
| author_sort | Seon-Ok Lee |
| collection | DOAJ |
| description | Background: We have previously reported the anti-hepatic lipogenic effect of fermented <i>Rhus verniciflua</i> stokes extract (FRVE) in an oleic-acid-treated HepG2 cell model. Methods: Herein, we advanced our understanding and evaluated the impact of FRVE in HFD-fed C57BL/6 mice using an animal model of nonalcoholic fatty liver disease (NAFLD). Milk thistle extract was used as a positive control to compare the effects of FRVE. Results: FRVE decreased body weight, intra-abdominal fat weight, and liver weight. Furthermore, FRVE decreased HFD-induced elevated serum levels of ALT, AST, TC, and TG, and downregulated the increase in hepatic lipid accumulation and TG levels. FRVE reduced hepatic SREBP-1, PCSK-9, SREBP-2, and ApoB mRNA levels. IHC data showed that FRVE reduced the levels of nucleic SREBP-1, increased the levels of LDLR, and upregulated the expression of p-AMPK. Conclusion: Overall, these results demonstrate the anti-hepatic lipidemic effect of FRVE in an animal model. These findings are consistent with our previous study and strongly suggest that FRVE exerts anti-hepatic lipogenic effects by activating AMPK. |
| first_indexed | 2024-03-10T15:58:15Z |
| format | Article |
| id | doaj.art-cdf687a0581a4d4dbcfded160c77a416 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T15:58:15Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-cdf687a0581a4d4dbcfded160c77a4162023-11-20T15:29:39ZengMDPI AGApplied Sciences2076-34172020-09-011019683310.3390/app10196833Fermented Rhus Verniciflua Stokes Extract Alleviates Nonalcoholic Fatty Liver through the AMPK/SREBP1/PCSK9 Pathway in HFD-Induced Nonalcoholic Fatty Liver Animal ModelSeon-Ok Lee0Yinzhu Xu1Hengmin Han2Seok-Tae Jeong3You-Kyung Lee4Jean Kyung Paik5Jin-Sol Cha6Hyo-Jeong Lee7Department of Science in Korean medicine, College of Korean Medicine, Graduate School, Kyung Hee University, Hoegi-dong, Dongdaemun-gu, Seoul 02435, KoreaDepartment of Science in Korean medicine, College of Korean Medicine, Graduate School, Kyung Hee University, Hoegi-dong, Dongdaemun-gu, Seoul 02435, KoreaDepartment of Cancer Preventive Material Development, College of Korean Medicine, Graduate School, Kyung Hee University, Hoegi-dong, Dongdaemun-gu, Seoul 02435, KoreaFermented Food Science Division, National Institute of Agricultural Sciences, 166, Nongsaengmyeongro, Iseo-myeon, WanjuGun, Jeollabuk-do 55365, KoreaDepartment of Cancer Preventive Material Development, College of Korean Medicine, Graduate School, Kyung Hee University, Hoegi-dong, Dongdaemun-gu, Seoul 02435, KoreaDepartment of Food and nutrition, Eulji University, Seongnam, Kyungido 13135, KoreaDepartment of Science in Korean medicine, College of Korean Medicine, Graduate School, Kyung Hee University, Hoegi-dong, Dongdaemun-gu, Seoul 02435, KoreaDepartment of Science in Korean medicine, College of Korean Medicine, Graduate School, Kyung Hee University, Hoegi-dong, Dongdaemun-gu, Seoul 02435, KoreaBackground: We have previously reported the anti-hepatic lipogenic effect of fermented <i>Rhus verniciflua</i> stokes extract (FRVE) in an oleic-acid-treated HepG2 cell model. Methods: Herein, we advanced our understanding and evaluated the impact of FRVE in HFD-fed C57BL/6 mice using an animal model of nonalcoholic fatty liver disease (NAFLD). Milk thistle extract was used as a positive control to compare the effects of FRVE. Results: FRVE decreased body weight, intra-abdominal fat weight, and liver weight. Furthermore, FRVE decreased HFD-induced elevated serum levels of ALT, AST, TC, and TG, and downregulated the increase in hepatic lipid accumulation and TG levels. FRVE reduced hepatic SREBP-1, PCSK-9, SREBP-2, and ApoB mRNA levels. IHC data showed that FRVE reduced the levels of nucleic SREBP-1, increased the levels of LDLR, and upregulated the expression of p-AMPK. Conclusion: Overall, these results demonstrate the anti-hepatic lipidemic effect of FRVE in an animal model. These findings are consistent with our previous study and strongly suggest that FRVE exerts anti-hepatic lipogenic effects by activating AMPK.https://www.mdpi.com/2076-3417/10/19/6833<i>Rhus verniciflua</i> stokesnonalcoholic fatty liver diseaseAMPKSREBP1HFD |
| spellingShingle | Seon-Ok Lee Yinzhu Xu Hengmin Han Seok-Tae Jeong You-Kyung Lee Jean Kyung Paik Jin-Sol Cha Hyo-Jeong Lee Fermented Rhus Verniciflua Stokes Extract Alleviates Nonalcoholic Fatty Liver through the AMPK/SREBP1/PCSK9 Pathway in HFD-Induced Nonalcoholic Fatty Liver Animal Model Applied Sciences <i>Rhus verniciflua</i> stokes nonalcoholic fatty liver disease AMPK SREBP1 HFD |
| title | Fermented Rhus Verniciflua Stokes Extract Alleviates Nonalcoholic Fatty Liver through the AMPK/SREBP1/PCSK9 Pathway in HFD-Induced Nonalcoholic Fatty Liver Animal Model |
| title_full | Fermented Rhus Verniciflua Stokes Extract Alleviates Nonalcoholic Fatty Liver through the AMPK/SREBP1/PCSK9 Pathway in HFD-Induced Nonalcoholic Fatty Liver Animal Model |
| title_fullStr | Fermented Rhus Verniciflua Stokes Extract Alleviates Nonalcoholic Fatty Liver through the AMPK/SREBP1/PCSK9 Pathway in HFD-Induced Nonalcoholic Fatty Liver Animal Model |
| title_full_unstemmed | Fermented Rhus Verniciflua Stokes Extract Alleviates Nonalcoholic Fatty Liver through the AMPK/SREBP1/PCSK9 Pathway in HFD-Induced Nonalcoholic Fatty Liver Animal Model |
| title_short | Fermented Rhus Verniciflua Stokes Extract Alleviates Nonalcoholic Fatty Liver through the AMPK/SREBP1/PCSK9 Pathway in HFD-Induced Nonalcoholic Fatty Liver Animal Model |
| title_sort | fermented rhus verniciflua stokes extract alleviates nonalcoholic fatty liver through the ampk srebp1 pcsk9 pathway in hfd induced nonalcoholic fatty liver animal model |
| topic | <i>Rhus verniciflua</i> stokes nonalcoholic fatty liver disease AMPK SREBP1 HFD |
| url | https://www.mdpi.com/2076-3417/10/19/6833 |
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