Regulation of Tomato Fruit Autophagic Flux and Promotion of Fruit Ripening by the Autophagy-Related Gene <i>SlATG8f</i>
Autophagy is a highly conserved self-degradation process that involves the degradation and recycling of cellular components and organelles. Although the involvement of autophagy in metabolic changes during fruit ripening has been preliminarily demonstrated, the variations in autophagic flux and spec...
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| Format: | Article |
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MDPI AG
2023-09-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/18/3339 |
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| author | Cen Wen Taimin Luo Zhuo He Yunzhou Li Jianmin Yan Wen Xu |
| author_facet | Cen Wen Taimin Luo Zhuo He Yunzhou Li Jianmin Yan Wen Xu |
| author_sort | Cen Wen |
| collection | DOAJ |
| description | Autophagy is a highly conserved self-degradation process that involves the degradation and recycling of cellular components and organelles. Although the involvement of autophagy in metabolic changes during fruit ripening has been preliminarily demonstrated, the variations in autophagic flux and specific functional roles in tomato fruit ripening remain to be elucidated. In this study, we analyzed the variations in autophagic flux during tomato fruit ripening. The results revealed differential expression of the <i>SlATG8</i> family members during tomato fruit ripening. Transmission electron microscopy observations and dansylcadaverine (MDC) staining confirmed the presence of autophagy at the cellular level in tomato fruits. Furthermore, the overexpression of <i>SlATG8f</i> induced the formation of autophagosomes, increased autophagic flux within tomato fruits, and effectively enhanced the expression of ATG8 proteins during the color-transition phase of fruit ripening, thus promoting tomato fruit maturation. <i>SlATG8f</i> overexpression also led to the accumulation of vitamin C (VC) and soluble solids while reducing acidity in the fruit. Collectively, our findings highlight the pivotal role of <i>SlATG8f</i> in enhancing tomato fruit ripening, providing insights into the mechanistic involvement of autophagy in this process. This research contributes to a better understanding of the key factors that regulate tomato fruit quality and offers a theoretical basis for tomato variety improvement. |
| first_indexed | 2024-03-10T22:12:46Z |
| format | Article |
| id | doaj.art-cdf755568dca45e19908987f1745307e |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T22:12:46Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-cdf755568dca45e19908987f1745307e2023-11-19T12:33:31ZengMDPI AGPlants2223-77472023-09-011218333910.3390/plants12183339Regulation of Tomato Fruit Autophagic Flux and Promotion of Fruit Ripening by the Autophagy-Related Gene <i>SlATG8f</i>Cen Wen0Taimin Luo1Zhuo He2Yunzhou Li3Jianmin Yan4Wen Xu5College of Agriculture, Guizhou University, Guizhou 550025, ChinaCollege of Agriculture, Guizhou University, Guizhou 550025, ChinaCollege of Agriculture, Guizhou University, Guizhou 550025, ChinaCollege of Agriculture, Guizhou University, Guizhou 550025, ChinaCollege of Agriculture, Guizhou University, Guizhou 550025, ChinaCollege of Agriculture, Guizhou University, Guizhou 550025, ChinaAutophagy is a highly conserved self-degradation process that involves the degradation and recycling of cellular components and organelles. Although the involvement of autophagy in metabolic changes during fruit ripening has been preliminarily demonstrated, the variations in autophagic flux and specific functional roles in tomato fruit ripening remain to be elucidated. In this study, we analyzed the variations in autophagic flux during tomato fruit ripening. The results revealed differential expression of the <i>SlATG8</i> family members during tomato fruit ripening. Transmission electron microscopy observations and dansylcadaverine (MDC) staining confirmed the presence of autophagy at the cellular level in tomato fruits. Furthermore, the overexpression of <i>SlATG8f</i> induced the formation of autophagosomes, increased autophagic flux within tomato fruits, and effectively enhanced the expression of ATG8 proteins during the color-transition phase of fruit ripening, thus promoting tomato fruit maturation. <i>SlATG8f</i> overexpression also led to the accumulation of vitamin C (VC) and soluble solids while reducing acidity in the fruit. Collectively, our findings highlight the pivotal role of <i>SlATG8f</i> in enhancing tomato fruit ripening, providing insights into the mechanistic involvement of autophagy in this process. This research contributes to a better understanding of the key factors that regulate tomato fruit quality and offers a theoretical basis for tomato variety improvement.https://www.mdpi.com/2223-7747/12/18/3339tomatofruit ripeningautophagosomeautophagic flux<i>SlATG8f</i> |
| spellingShingle | Cen Wen Taimin Luo Zhuo He Yunzhou Li Jianmin Yan Wen Xu Regulation of Tomato Fruit Autophagic Flux and Promotion of Fruit Ripening by the Autophagy-Related Gene <i>SlATG8f</i> Plants tomato fruit ripening autophagosome autophagic flux <i>SlATG8f</i> |
| title | Regulation of Tomato Fruit Autophagic Flux and Promotion of Fruit Ripening by the Autophagy-Related Gene <i>SlATG8f</i> |
| title_full | Regulation of Tomato Fruit Autophagic Flux and Promotion of Fruit Ripening by the Autophagy-Related Gene <i>SlATG8f</i> |
| title_fullStr | Regulation of Tomato Fruit Autophagic Flux and Promotion of Fruit Ripening by the Autophagy-Related Gene <i>SlATG8f</i> |
| title_full_unstemmed | Regulation of Tomato Fruit Autophagic Flux and Promotion of Fruit Ripening by the Autophagy-Related Gene <i>SlATG8f</i> |
| title_short | Regulation of Tomato Fruit Autophagic Flux and Promotion of Fruit Ripening by the Autophagy-Related Gene <i>SlATG8f</i> |
| title_sort | regulation of tomato fruit autophagic flux and promotion of fruit ripening by the autophagy related gene i slatg8f i |
| topic | tomato fruit ripening autophagosome autophagic flux <i>SlATG8f</i> |
| url | https://www.mdpi.com/2223-7747/12/18/3339 |
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