Overexpression of the Selective Autophagy Cargo Receptor NBR1 Modifies Plant Response to Sulfur Deficit
Plants exposed to sulfur deficit elevate the transcription of <i>NBR1</i> what might reflect an increased demand for NBR1 in such conditions. Therefore, we investigated the role of this selective autophagy cargo receptor in plant response to sulfur deficit (-S). Transcriptome analysis of...
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MDPI AG
2020-03-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/9/3/669 |
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| author | Leszek Tarnowski Milagros Collados Rodriguez Jerzy Brzywczy Dominik Cysewski Anna Wawrzynska Agnieszka Sirko |
| author_facet | Leszek Tarnowski Milagros Collados Rodriguez Jerzy Brzywczy Dominik Cysewski Anna Wawrzynska Agnieszka Sirko |
| author_sort | Leszek Tarnowski |
| collection | DOAJ |
| description | Plants exposed to sulfur deficit elevate the transcription of <i>NBR1</i> what might reflect an increased demand for NBR1 in such conditions. Therefore, we investigated the role of this selective autophagy cargo receptor in plant response to sulfur deficit (-S). Transcriptome analysis of the wild type and NBR1 overexpressing plants pointed out differences in gene expression in response to -S. Our attention focused particularly on the genes upregulated by -S in roots of both lines because of significant overrepresentation of cytoplasmic ribosomal gene family. Moreover, we noticed overrepresentation of the same family in the set of proteins co-purifying with NBR1 in -S. One of these ribosomal proteins, RPS6 was chosen for verification of its direct interaction with NBR1 and proven to bind outside the NBR1 ubiquitin binding domains. The biological significance of this novel interaction and the postulated role of NBR1 in ribosomes remodeling in response to starvation remain to be further investigated. Interestingly, NBR1 overexpressing seedlings have significantly shorter roots than wild type when grown in nutrient deficient conditions in the presence of TOR kinase inhibitors. This phenotype probably results from excessive autophagy induction by the additive effect of NBR1 overexpression, starvation, and TOR inhibition. |
| first_indexed | 2024-03-12T09:58:46Z |
| format | Article |
| id | doaj.art-cdf88018bdd645d68ae1f3547a0481bb |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-12T09:58:46Z |
| publishDate | 2020-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-cdf88018bdd645d68ae1f3547a0481bb2023-09-02T12:00:25ZengMDPI AGCells2073-44092020-03-019366910.3390/cells9030669cells9030669Overexpression of the Selective Autophagy Cargo Receptor NBR1 Modifies Plant Response to Sulfur DeficitLeszek Tarnowski0Milagros Collados Rodriguez1Jerzy Brzywczy2Dominik Cysewski3Anna Wawrzynska4Agnieszka Sirko5Department of Plant Biochemistry, Institute of Biochemistry and Biophysics Polish Academy of Sciences, Pawińskiego 5A St, 02-106 Warsaw, PolandDepartment of Plant Biochemistry, Institute of Biochemistry and Biophysics Polish Academy of Sciences, Pawińskiego 5A St, 02-106 Warsaw, PolandDepartment of Plant Biochemistry, Institute of Biochemistry and Biophysics Polish Academy of Sciences, Pawińskiego 5A St, 02-106 Warsaw, PolandLaboratory of Mass Spectrometry, Institute of Biochemistry and Biophysics Polish Academy of Sciences, Pawińskiego 5A St, 02-106 Warsaw, PolandDepartment of Plant Biochemistry, Institute of Biochemistry and Biophysics Polish Academy of Sciences, Pawińskiego 5A St, 02-106 Warsaw, PolandDepartment of Plant Biochemistry, Institute of Biochemistry and Biophysics Polish Academy of Sciences, Pawińskiego 5A St, 02-106 Warsaw, PolandPlants exposed to sulfur deficit elevate the transcription of <i>NBR1</i> what might reflect an increased demand for NBR1 in such conditions. Therefore, we investigated the role of this selective autophagy cargo receptor in plant response to sulfur deficit (-S). Transcriptome analysis of the wild type and NBR1 overexpressing plants pointed out differences in gene expression in response to -S. Our attention focused particularly on the genes upregulated by -S in roots of both lines because of significant overrepresentation of cytoplasmic ribosomal gene family. Moreover, we noticed overrepresentation of the same family in the set of proteins co-purifying with NBR1 in -S. One of these ribosomal proteins, RPS6 was chosen for verification of its direct interaction with NBR1 and proven to bind outside the NBR1 ubiquitin binding domains. The biological significance of this novel interaction and the postulated role of NBR1 in ribosomes remodeling in response to starvation remain to be further investigated. Interestingly, NBR1 overexpressing seedlings have significantly shorter roots than wild type when grown in nutrient deficient conditions in the presence of TOR kinase inhibitors. This phenotype probably results from excessive autophagy induction by the additive effect of NBR1 overexpression, starvation, and TOR inhibition.https://www.mdpi.com/2073-4409/9/3/669autophagyprotein-protein interactionsulfur deficittranscriptome<i>arabidopsis thaliana</i> |
| spellingShingle | Leszek Tarnowski Milagros Collados Rodriguez Jerzy Brzywczy Dominik Cysewski Anna Wawrzynska Agnieszka Sirko Overexpression of the Selective Autophagy Cargo Receptor NBR1 Modifies Plant Response to Sulfur Deficit Cells autophagy protein-protein interaction sulfur deficit transcriptome <i>arabidopsis thaliana</i> |
| title | Overexpression of the Selective Autophagy Cargo Receptor NBR1 Modifies Plant Response to Sulfur Deficit |
| title_full | Overexpression of the Selective Autophagy Cargo Receptor NBR1 Modifies Plant Response to Sulfur Deficit |
| title_fullStr | Overexpression of the Selective Autophagy Cargo Receptor NBR1 Modifies Plant Response to Sulfur Deficit |
| title_full_unstemmed | Overexpression of the Selective Autophagy Cargo Receptor NBR1 Modifies Plant Response to Sulfur Deficit |
| title_short | Overexpression of the Selective Autophagy Cargo Receptor NBR1 Modifies Plant Response to Sulfur Deficit |
| title_sort | overexpression of the selective autophagy cargo receptor nbr1 modifies plant response to sulfur deficit |
| topic | autophagy protein-protein interaction sulfur deficit transcriptome <i>arabidopsis thaliana</i> |
| url | https://www.mdpi.com/2073-4409/9/3/669 |
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