RNA-binding proteins to assess gene expression states of co-cultivated cells in response to tumor cells
<p>Abstract</p> <p>Background</p> <p>Tumors and complex tissues consist of mixtures of communicating cells that differ significantly in their gene expression status. In order to understand how different cell types influence one another's gene expression, it will be...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2004-09-01
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| Series: | Molecular Cancer |
| Online Access: | http://www.molecular-cancer.com/content/3/1/24 |
| _version_ | 1818392249514328064 |
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| author | Penalva Luiz OF Burdick Michael D Lin Simon M Sutterluety Hedwig Keene Jack D |
| author_facet | Penalva Luiz OF Burdick Michael D Lin Simon M Sutterluety Hedwig Keene Jack D |
| author_sort | Penalva Luiz OF |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Tumors and complex tissues consist of mixtures of communicating cells that differ significantly in their gene expression status. In order to understand how different cell types influence one another's gene expression, it will be necessary to monitor the mRNA profiles of each cell type independently and to dissect the mechanisms that regulate their gene expression outcomes.</p> <p>Results</p> <p>In order to approach these questions, we have used RNA-binding proteins such as ELAV/Hu, poly (A) binding protein (PABP) and cap-binding protein (eIF-4E) as reporters of gene expression. Here we demonstrate that the epitope-tagged RNA binding protein, PABP, expressed separately in tumor cells and endothelial cells can be used to discriminate their respective mRNA targets from mixtures of these cells without significant mRNA reassortment or exchange. Moreover, using this approach we identify a set of endothelial genes that respond to the presence of co-cultured breast tumor cells.</p> <p>Conclusion</p> <p>RNA-binding proteins can be used as reporters to elucidate components of operational mRNA networks and operons involved in regulating cell-type specific gene expression in tissues and tumors.</p> |
| first_indexed | 2024-12-14T05:26:25Z |
| format | Article |
| id | doaj.art-cdfbed83112c456aa8a0b98e9760db4f |
| institution | Directory Open Access Journal |
| issn | 1476-4598 |
| language | English |
| last_indexed | 2024-12-14T05:26:25Z |
| publishDate | 2004-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Molecular Cancer |
| spelling | doaj.art-cdfbed83112c456aa8a0b98e9760db4f2022-12-21T23:15:31ZengBMCMolecular Cancer1476-45982004-09-01312410.1186/1476-4598-3-24RNA-binding proteins to assess gene expression states of co-cultivated cells in response to tumor cellsPenalva Luiz OFBurdick Michael DLin Simon MSutterluety HedwigKeene Jack D<p>Abstract</p> <p>Background</p> <p>Tumors and complex tissues consist of mixtures of communicating cells that differ significantly in their gene expression status. In order to understand how different cell types influence one another's gene expression, it will be necessary to monitor the mRNA profiles of each cell type independently and to dissect the mechanisms that regulate their gene expression outcomes.</p> <p>Results</p> <p>In order to approach these questions, we have used RNA-binding proteins such as ELAV/Hu, poly (A) binding protein (PABP) and cap-binding protein (eIF-4E) as reporters of gene expression. Here we demonstrate that the epitope-tagged RNA binding protein, PABP, expressed separately in tumor cells and endothelial cells can be used to discriminate their respective mRNA targets from mixtures of these cells without significant mRNA reassortment or exchange. Moreover, using this approach we identify a set of endothelial genes that respond to the presence of co-cultured breast tumor cells.</p> <p>Conclusion</p> <p>RNA-binding proteins can be used as reporters to elucidate components of operational mRNA networks and operons involved in regulating cell-type specific gene expression in tissues and tumors.</p>http://www.molecular-cancer.com/content/3/1/24 |
| spellingShingle | Penalva Luiz OF Burdick Michael D Lin Simon M Sutterluety Hedwig Keene Jack D RNA-binding proteins to assess gene expression states of co-cultivated cells in response to tumor cells Molecular Cancer |
| title | RNA-binding proteins to assess gene expression states of co-cultivated cells in response to tumor cells |
| title_full | RNA-binding proteins to assess gene expression states of co-cultivated cells in response to tumor cells |
| title_fullStr | RNA-binding proteins to assess gene expression states of co-cultivated cells in response to tumor cells |
| title_full_unstemmed | RNA-binding proteins to assess gene expression states of co-cultivated cells in response to tumor cells |
| title_short | RNA-binding proteins to assess gene expression states of co-cultivated cells in response to tumor cells |
| title_sort | rna binding proteins to assess gene expression states of co cultivated cells in response to tumor cells |
| url | http://www.molecular-cancer.com/content/3/1/24 |
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