A Compact Closed-Loop Optogenetics System Based on Artifact-Free Transparent Graphene Electrodes
Electrophysiology is a decades-old technique widely used for monitoring activity of individual neurons and local field potentials. Optogenetics has revolutionized neuroscience studies by offering selective and fast control of targeted neurons and neuron populations. The combination of these two tech...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-03-01
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| Series: | Frontiers in Neuroscience |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/article/10.3389/fnins.2018.00132/full |
| _version_ | 1819259208557658112 |
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| author | Xin Liu Yichen Lu Ege Iseri Yuhan Shi Duygu Kuzum |
| author_facet | Xin Liu Yichen Lu Ege Iseri Yuhan Shi Duygu Kuzum |
| author_sort | Xin Liu |
| collection | DOAJ |
| description | Electrophysiology is a decades-old technique widely used for monitoring activity of individual neurons and local field potentials. Optogenetics has revolutionized neuroscience studies by offering selective and fast control of targeted neurons and neuron populations. The combination of these two techniques is crucial for causal investigation of neural circuits and understanding their functional connectivity. However, electrical artifacts generated by light stimulation interfere with neural recordings and hinder the development of compact closed-loop systems for precise control of neural activity. Here, we demonstrate that transparent graphene micro-electrodes fabricated on a clear polyethylene terephthalate film eliminate the light-induced artifact problem and allow development of a compact battery-powered closed-loop optogenetics system. We extensively investigate light-induced artifacts for graphene electrodes in comparison to metal control electrodes. We then design optical stimulation module using micro-LED chips coupled to optical fibers to deliver light to intended depth for optogenetic stimulation. For artifact-free integration of graphene micro-electrode recordings with optogenetic stimulation, we design and develop a compact closed-loop system and validate it for different frequencies of interest for neural recordings. This compact closed-loop optogenetics system can be used for various applications involving optogenetic stimulation and electrophysiological recordings. |
| first_indexed | 2024-12-23T19:06:22Z |
| format | Article |
| id | doaj.art-cdfc73fd55b6406ca8b79634241c1ba3 |
| institution | Directory Open Access Journal |
| issn | 1662-453X |
| language | English |
| last_indexed | 2024-12-23T19:06:22Z |
| publishDate | 2018-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neuroscience |
| spelling | doaj.art-cdfc73fd55b6406ca8b79634241c1ba32022-12-21T17:34:36ZengFrontiers Media S.A.Frontiers in Neuroscience1662-453X2018-03-011210.3389/fnins.2018.00132322176A Compact Closed-Loop Optogenetics System Based on Artifact-Free Transparent Graphene ElectrodesXin LiuYichen LuEge IseriYuhan ShiDuygu KuzumElectrophysiology is a decades-old technique widely used for monitoring activity of individual neurons and local field potentials. Optogenetics has revolutionized neuroscience studies by offering selective and fast control of targeted neurons and neuron populations. The combination of these two techniques is crucial for causal investigation of neural circuits and understanding their functional connectivity. However, electrical artifacts generated by light stimulation interfere with neural recordings and hinder the development of compact closed-loop systems for precise control of neural activity. Here, we demonstrate that transparent graphene micro-electrodes fabricated on a clear polyethylene terephthalate film eliminate the light-induced artifact problem and allow development of a compact battery-powered closed-loop optogenetics system. We extensively investigate light-induced artifacts for graphene electrodes in comparison to metal control electrodes. We then design optical stimulation module using micro-LED chips coupled to optical fibers to deliver light to intended depth for optogenetic stimulation. For artifact-free integration of graphene micro-electrode recordings with optogenetic stimulation, we design and develop a compact closed-loop system and validate it for different frequencies of interest for neural recordings. This compact closed-loop optogenetics system can be used for various applications involving optogenetic stimulation and electrophysiological recordings.http://journal.frontiersin.org/article/10.3389/fnins.2018.00132/fulloptogeneticsgrapheneclosed-loop optogeneticstransparent graphene arraymulti-electrode arraylight-induced artifact |
| spellingShingle | Xin Liu Yichen Lu Ege Iseri Yuhan Shi Duygu Kuzum A Compact Closed-Loop Optogenetics System Based on Artifact-Free Transparent Graphene Electrodes Frontiers in Neuroscience optogenetics graphene closed-loop optogenetics transparent graphene array multi-electrode array light-induced artifact |
| title | A Compact Closed-Loop Optogenetics System Based on Artifact-Free Transparent Graphene Electrodes |
| title_full | A Compact Closed-Loop Optogenetics System Based on Artifact-Free Transparent Graphene Electrodes |
| title_fullStr | A Compact Closed-Loop Optogenetics System Based on Artifact-Free Transparent Graphene Electrodes |
| title_full_unstemmed | A Compact Closed-Loop Optogenetics System Based on Artifact-Free Transparent Graphene Electrodes |
| title_short | A Compact Closed-Loop Optogenetics System Based on Artifact-Free Transparent Graphene Electrodes |
| title_sort | compact closed loop optogenetics system based on artifact free transparent graphene electrodes |
| topic | optogenetics graphene closed-loop optogenetics transparent graphene array multi-electrode array light-induced artifact |
| url | http://journal.frontiersin.org/article/10.3389/fnins.2018.00132/full |
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