Environmental DNA dynamics during embryonic development in the rare minnow Gobiocypris rarus
Abstract Understanding environmental DNA (eDNA) dynamics in the major developmental stages of fish species is fundamental for developing eDNA methods to aid conservation and management of fish species and/or populations. In this study, we used the model fish species, the rare minnow (Gobiocypris rar...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-11-01
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| Series: | Environmental DNA |
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| Online Access: | https://doi.org/10.1002/edn3.339 |
| _version_ | 1811218234502807552 |
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| author | Lu Shu Zuogang Peng |
| author_facet | Lu Shu Zuogang Peng |
| author_sort | Lu Shu |
| collection | DOAJ |
| description | Abstract Understanding environmental DNA (eDNA) dynamics in the major developmental stages of fish species is fundamental for developing eDNA methods to aid conservation and management of fish species and/or populations. In this study, we used the model fish species, the rare minnow (Gobiocypris rarus), to assess the temporal changes of its mitochondrial and nuclear eDNA concentrations during its embryonic development in a culture dish experiment. Mitochondrial cytochrome b (Cytb) and nuclear internal transcribed spacer‐1 (ITS1), considered as representative DNA fragments, were amplified by quantitative PCR (qPCR). In this embryo culture trial, spikes in mitochondrial (mt‐) and nuclear (nu‐) eDNA concentrations were found just after egg fertilization and after embryo hatching, and a modest but positive relationship was found between the eDNA concentration and the number of hatched individuals, illustrating eDNA may be used to monitor the spawning and hatching events of externally fertilizing fishes. These findings provided basic information on eDNA dynamics during fish embryonic development and a new insight to design eDNA methods to monitor early developmental stages of fish species. |
| first_indexed | 2024-04-12T07:07:38Z |
| format | Article |
| id | doaj.art-cdfe8aea78df41ddbe9cdf0f1f694e80 |
| institution | Directory Open Access Journal |
| issn | 2637-4943 |
| language | English |
| last_indexed | 2024-04-12T07:07:38Z |
| publishDate | 2022-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Environmental DNA |
| spelling | doaj.art-cdfe8aea78df41ddbe9cdf0f1f694e802022-12-22T03:42:45ZengWileyEnvironmental DNA2637-49432022-11-01461284129310.1002/edn3.339Environmental DNA dynamics during embryonic development in the rare minnow Gobiocypris rarusLu Shu0Zuogang Peng1Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education) Southwest University School of Life Sciences Chongqing ChinaKey Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education) Southwest University School of Life Sciences Chongqing ChinaAbstract Understanding environmental DNA (eDNA) dynamics in the major developmental stages of fish species is fundamental for developing eDNA methods to aid conservation and management of fish species and/or populations. In this study, we used the model fish species, the rare minnow (Gobiocypris rarus), to assess the temporal changes of its mitochondrial and nuclear eDNA concentrations during its embryonic development in a culture dish experiment. Mitochondrial cytochrome b (Cytb) and nuclear internal transcribed spacer‐1 (ITS1), considered as representative DNA fragments, were amplified by quantitative PCR (qPCR). In this embryo culture trial, spikes in mitochondrial (mt‐) and nuclear (nu‐) eDNA concentrations were found just after egg fertilization and after embryo hatching, and a modest but positive relationship was found between the eDNA concentration and the number of hatched individuals, illustrating eDNA may be used to monitor the spawning and hatching events of externally fertilizing fishes. These findings provided basic information on eDNA dynamics during fish embryonic development and a new insight to design eDNA methods to monitor early developmental stages of fish species.https://doi.org/10.1002/edn3.339CytbeDNA dynamicsembryonic developmentenvironmental DNAGobiocypris rarusITS1 |
| spellingShingle | Lu Shu Zuogang Peng Environmental DNA dynamics during embryonic development in the rare minnow Gobiocypris rarus Environmental DNA Cytb eDNA dynamics embryonic development environmental DNA Gobiocypris rarus ITS1 |
| title | Environmental DNA dynamics during embryonic development in the rare minnow Gobiocypris rarus |
| title_full | Environmental DNA dynamics during embryonic development in the rare minnow Gobiocypris rarus |
| title_fullStr | Environmental DNA dynamics during embryonic development in the rare minnow Gobiocypris rarus |
| title_full_unstemmed | Environmental DNA dynamics during embryonic development in the rare minnow Gobiocypris rarus |
| title_short | Environmental DNA dynamics during embryonic development in the rare minnow Gobiocypris rarus |
| title_sort | environmental dna dynamics during embryonic development in the rare minnow gobiocypris rarus |
| topic | Cytb eDNA dynamics embryonic development environmental DNA Gobiocypris rarus ITS1 |
| url | https://doi.org/10.1002/edn3.339 |
| work_keys_str_mv | AT lushu environmentaldnadynamicsduringembryonicdevelopmentintherareminnowgobiocyprisrarus AT zuogangpeng environmentaldnadynamicsduringembryonicdevelopmentintherareminnowgobiocyprisrarus |