Exopolysaccharide Produced by <i>Lactiplantibacillus plantarum</i>-12 Alleviates Intestinal Inflammation and Colon Cancer Symptoms by Modulating the Gut Microbiome and Metabolites of C57BL/6 Mice Treated by Azoxymethane/Dextran Sulfate Sodium Salt
Exopolysaccharide produced by <i>Lactiplantibacillus plantarum</i>-12 (LPEPS) exhibited the anti-proliferating effect on human colon cancer cell line HT-29 in vitro. The purpose of the study was to determine the alleviating effects of LPEPS on colon cancer development of the C57BL/6 mice...
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MDPI AG
2021-12-01
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| Series: | Foods |
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| author | Fenglian Ma Yinglong Song Mengying Sun Arong Wang Shujuan Jiang Guangqing Mu Yanfeng Tuo |
| author_facet | Fenglian Ma Yinglong Song Mengying Sun Arong Wang Shujuan Jiang Guangqing Mu Yanfeng Tuo |
| author_sort | Fenglian Ma |
| collection | DOAJ |
| description | Exopolysaccharide produced by <i>Lactiplantibacillus plantarum</i>-12 (LPEPS) exhibited the anti-proliferating effect on human colon cancer cell line HT-29 in vitro. The purpose of the study was to determine the alleviating effects of LPEPS on colon cancer development of the C57BL/6 mice treated by azoxymethane/dextran sulfate sodium salt (AOM/DSS). The C57BL/6 mice treated by AOM/DSS were orally administered LPEPS daily for 85 days. The results showed that LPEPS oral administration enhanced colon tight-junction protein expression and ameliorated colon shortening and tumor burden of the AOM/DSS treated mice. Furthermore, LPEPS oral administration significantly reduced pro-inflammatory factors TNF-α, IL-8, and IL-1β levels and increased anti-inflammatory factor IL-10 level in the serum of the AOM/DSS-treated mice. LPEPS oral administration reversed the alterations of gut flora in AOM/DSS-treated mice, as evidenced by the increasing of the abundance of <i>Bacteroidetes</i>, <i>Bacteroidetes</i>/<i>Firmicutes</i> ratio, <i>Muribaculaceae</i>, <i>Burkholderiaceae</i>, and <i>norank_o__Rhodospirillales</i> and the decreasing of the abundance of <i>Firmicutes</i>, <i>Desulfovibrionaceae</i>, <i>Erysipelotrichaceae</i>, and <i>Helicobacteraceae</i>. The fecal metabolites of the AOM/DSS-treated mice were altered by LPEPS oral administration, involving lipid metabolism and amino acid metabolism. Together, these results suggested that LPEPS oral administration alleviated AOM/DSS-induced colon cancer symptoms of the C57BL/6 mice by modulating gut microbiota and metabolites, enhancing intestine barrier, inhibiting NF-κB pathway, and activating caspase cascade. |
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| language | English |
| last_indexed | 2024-03-10T04:08:34Z |
| publishDate | 2021-12-01 |
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| spelling | doaj.art-ce035e9bcdfa442fbe8f3eea3304ce852023-11-23T08:18:16ZengMDPI AGFoods2304-81582021-12-011012306010.3390/foods10123060Exopolysaccharide Produced by <i>Lactiplantibacillus plantarum</i>-12 Alleviates Intestinal Inflammation and Colon Cancer Symptoms by Modulating the Gut Microbiome and Metabolites of C57BL/6 Mice Treated by Azoxymethane/Dextran Sulfate Sodium SaltFenglian Ma0Yinglong Song1Mengying Sun2Arong Wang3Shujuan Jiang4Guangqing Mu5Yanfeng Tuo6School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, ChinaSchool of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, ChinaSchool of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, ChinaSchool of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, ChinaSchool of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, ChinaSchool of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, ChinaSchool of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, ChinaExopolysaccharide produced by <i>Lactiplantibacillus plantarum</i>-12 (LPEPS) exhibited the anti-proliferating effect on human colon cancer cell line HT-29 in vitro. The purpose of the study was to determine the alleviating effects of LPEPS on colon cancer development of the C57BL/6 mice treated by azoxymethane/dextran sulfate sodium salt (AOM/DSS). The C57BL/6 mice treated by AOM/DSS were orally administered LPEPS daily for 85 days. The results showed that LPEPS oral administration enhanced colon tight-junction protein expression and ameliorated colon shortening and tumor burden of the AOM/DSS treated mice. Furthermore, LPEPS oral administration significantly reduced pro-inflammatory factors TNF-α, IL-8, and IL-1β levels and increased anti-inflammatory factor IL-10 level in the serum of the AOM/DSS-treated mice. LPEPS oral administration reversed the alterations of gut flora in AOM/DSS-treated mice, as evidenced by the increasing of the abundance of <i>Bacteroidetes</i>, <i>Bacteroidetes</i>/<i>Firmicutes</i> ratio, <i>Muribaculaceae</i>, <i>Burkholderiaceae</i>, and <i>norank_o__Rhodospirillales</i> and the decreasing of the abundance of <i>Firmicutes</i>, <i>Desulfovibrionaceae</i>, <i>Erysipelotrichaceae</i>, and <i>Helicobacteraceae</i>. The fecal metabolites of the AOM/DSS-treated mice were altered by LPEPS oral administration, involving lipid metabolism and amino acid metabolism. Together, these results suggested that LPEPS oral administration alleviated AOM/DSS-induced colon cancer symptoms of the C57BL/6 mice by modulating gut microbiota and metabolites, enhancing intestine barrier, inhibiting NF-κB pathway, and activating caspase cascade.https://www.mdpi.com/2304-8158/10/12/3060exopolysaccharide<i>Lactiplantibacillus plantarum</i>gut microbiotametabolitecolon cancer |
| spellingShingle | Fenglian Ma Yinglong Song Mengying Sun Arong Wang Shujuan Jiang Guangqing Mu Yanfeng Tuo Exopolysaccharide Produced by <i>Lactiplantibacillus plantarum</i>-12 Alleviates Intestinal Inflammation and Colon Cancer Symptoms by Modulating the Gut Microbiome and Metabolites of C57BL/6 Mice Treated by Azoxymethane/Dextran Sulfate Sodium Salt Foods exopolysaccharide <i>Lactiplantibacillus plantarum</i> gut microbiota metabolite colon cancer |
| title | Exopolysaccharide Produced by <i>Lactiplantibacillus plantarum</i>-12 Alleviates Intestinal Inflammation and Colon Cancer Symptoms by Modulating the Gut Microbiome and Metabolites of C57BL/6 Mice Treated by Azoxymethane/Dextran Sulfate Sodium Salt |
| title_full | Exopolysaccharide Produced by <i>Lactiplantibacillus plantarum</i>-12 Alleviates Intestinal Inflammation and Colon Cancer Symptoms by Modulating the Gut Microbiome and Metabolites of C57BL/6 Mice Treated by Azoxymethane/Dextran Sulfate Sodium Salt |
| title_fullStr | Exopolysaccharide Produced by <i>Lactiplantibacillus plantarum</i>-12 Alleviates Intestinal Inflammation and Colon Cancer Symptoms by Modulating the Gut Microbiome and Metabolites of C57BL/6 Mice Treated by Azoxymethane/Dextran Sulfate Sodium Salt |
| title_full_unstemmed | Exopolysaccharide Produced by <i>Lactiplantibacillus plantarum</i>-12 Alleviates Intestinal Inflammation and Colon Cancer Symptoms by Modulating the Gut Microbiome and Metabolites of C57BL/6 Mice Treated by Azoxymethane/Dextran Sulfate Sodium Salt |
| title_short | Exopolysaccharide Produced by <i>Lactiplantibacillus plantarum</i>-12 Alleviates Intestinal Inflammation and Colon Cancer Symptoms by Modulating the Gut Microbiome and Metabolites of C57BL/6 Mice Treated by Azoxymethane/Dextran Sulfate Sodium Salt |
| title_sort | exopolysaccharide produced by i lactiplantibacillus plantarum i 12 alleviates intestinal inflammation and colon cancer symptoms by modulating the gut microbiome and metabolites of c57bl 6 mice treated by azoxymethane dextran sulfate sodium salt |
| topic | exopolysaccharide <i>Lactiplantibacillus plantarum</i> gut microbiota metabolite colon cancer |
| url | https://www.mdpi.com/2304-8158/10/12/3060 |
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