Emergent Molecular Techniques Applied to the Detection of Porcine Viruses
Molecular diagnostic tests have evolved very rapidly in the field of human health, especially with the arrival of the recent pandemic caused by the SARS-CoV-2 virus. However, the animal sector is constantly neglected, even though accurate detection by molecular tools could represent economic advanta...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-10-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/10/10/609 |
| _version_ | 1797572141042892800 |
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| author | Elda A. Flores-Contreras Jorge Alberto Carrasco-González Daniel C. L. Linhares Cesar A. Corzo J. Israel Campos-Villalobos Alexandra Henao-Díaz Elda M. Melchor-Martínez Hafiz M. N. Iqbal Reyna Berenice González-González Roberto Parra-Saldívar Everardo González-González |
| author_facet | Elda A. Flores-Contreras Jorge Alberto Carrasco-González Daniel C. L. Linhares Cesar A. Corzo J. Israel Campos-Villalobos Alexandra Henao-Díaz Elda M. Melchor-Martínez Hafiz M. N. Iqbal Reyna Berenice González-González Roberto Parra-Saldívar Everardo González-González |
| author_sort | Elda A. Flores-Contreras |
| collection | DOAJ |
| description | Molecular diagnostic tests have evolved very rapidly in the field of human health, especially with the arrival of the recent pandemic caused by the SARS-CoV-2 virus. However, the animal sector is constantly neglected, even though accurate detection by molecular tools could represent economic advantages by preventing the spread of viruses. In this regard, the swine industry is of great interest. The main viruses that affect the swine industry are described in this review, including African swine fever virus (ASFV), porcine reproductive and respiratory syndrome virus (PRRSV), porcine epidemic diarrhea virus (PEDV), and porcine circovirus (PCV), which have been effectively detected by different molecular tools in recent times. Here, we describe the rationale of molecular techniques such as multiplex PCR, isothermal methods (LAMP, NASBA, RPA, and PSR) and novel methods such as CRISPR-Cas and microfluidics platforms. Successful molecular diagnostic developments are presented by highlighting their most important findings. Finally, we describe the barriers that hinder the large-scale development of affordable, accessible, rapid, and easy-to-use molecular diagnostic tests. The evolution of diagnostic techniques is critical to prevent the spread of viruses and the development of viral reservoirs in the swine industry that impact the possible development of future pandemics and the world economy. |
| first_indexed | 2024-03-10T20:50:16Z |
| format | Article |
| id | doaj.art-ce04e2ae821742c6ad1a55a29017066d |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-10T20:50:16Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-ce04e2ae821742c6ad1a55a29017066d2023-11-19T18:25:56ZengMDPI AGVeterinary Sciences2306-73812023-10-01101060910.3390/vetsci10100609Emergent Molecular Techniques Applied to the Detection of Porcine VirusesElda A. Flores-Contreras0Jorge Alberto Carrasco-González1Daniel C. L. Linhares2Cesar A. Corzo3J. Israel Campos-Villalobos4Alexandra Henao-Díaz5Elda M. Melchor-Martínez6Hafiz M. N. Iqbal7Reyna Berenice González-González8Roberto Parra-Saldívar9Everardo González-González10Tecnologico de Monterrey, School of Engineering and Sciences, Monterrey 64849, Nuevo Leon, MexicoBiosafety, Lomas de los Pinos No. 5505—F, Col. La Estanzuela vieja, Monterrey 64984, Nuevo Leon, MexicoVeterinary Diagnostic and Production Animal Medicine Department, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USAVeterinary Population Medicine Department, College of Veterinary Medicine, University of Minnesota, St. Paul, MN 55455, USAProteina Animal S.A. de C.V. (PROAN), San Juan de los Lagos 47000, Jalisco, MexicoIndustrias Bachoco, S.A. de C.V., Celaya, Celaya 38010, Guanajuato, MexicoTecnologico de Monterrey, School of Engineering and Sciences, Monterrey 64849, Nuevo Leon, MexicoTecnologico de Monterrey, School of Engineering and Sciences, Monterrey 64849, Nuevo Leon, MexicoTecnologico de Monterrey, School of Engineering and Sciences, Monterrey 64849, Nuevo Leon, MexicoTecnologico de Monterrey, School of Engineering and Sciences, Monterrey 64849, Nuevo Leon, MexicoTecnologico de Monterrey, School of Engineering and Sciences, Monterrey 64849, Nuevo Leon, MexicoMolecular diagnostic tests have evolved very rapidly in the field of human health, especially with the arrival of the recent pandemic caused by the SARS-CoV-2 virus. However, the animal sector is constantly neglected, even though accurate detection by molecular tools could represent economic advantages by preventing the spread of viruses. In this regard, the swine industry is of great interest. The main viruses that affect the swine industry are described in this review, including African swine fever virus (ASFV), porcine reproductive and respiratory syndrome virus (PRRSV), porcine epidemic diarrhea virus (PEDV), and porcine circovirus (PCV), which have been effectively detected by different molecular tools in recent times. Here, we describe the rationale of molecular techniques such as multiplex PCR, isothermal methods (LAMP, NASBA, RPA, and PSR) and novel methods such as CRISPR-Cas and microfluidics platforms. Successful molecular diagnostic developments are presented by highlighting their most important findings. Finally, we describe the barriers that hinder the large-scale development of affordable, accessible, rapid, and easy-to-use molecular diagnostic tests. The evolution of diagnostic techniques is critical to prevent the spread of viruses and the development of viral reservoirs in the swine industry that impact the possible development of future pandemics and the world economy.https://www.mdpi.com/2306-7381/10/10/609PCRLAMPRPANASBAPSRASFV |
| spellingShingle | Elda A. Flores-Contreras Jorge Alberto Carrasco-González Daniel C. L. Linhares Cesar A. Corzo J. Israel Campos-Villalobos Alexandra Henao-Díaz Elda M. Melchor-Martínez Hafiz M. N. Iqbal Reyna Berenice González-González Roberto Parra-Saldívar Everardo González-González Emergent Molecular Techniques Applied to the Detection of Porcine Viruses Veterinary Sciences PCR LAMP RPA NASBA PSR ASFV |
| title | Emergent Molecular Techniques Applied to the Detection of Porcine Viruses |
| title_full | Emergent Molecular Techniques Applied to the Detection of Porcine Viruses |
| title_fullStr | Emergent Molecular Techniques Applied to the Detection of Porcine Viruses |
| title_full_unstemmed | Emergent Molecular Techniques Applied to the Detection of Porcine Viruses |
| title_short | Emergent Molecular Techniques Applied to the Detection of Porcine Viruses |
| title_sort | emergent molecular techniques applied to the detection of porcine viruses |
| topic | PCR LAMP RPA NASBA PSR ASFV |
| url | https://www.mdpi.com/2306-7381/10/10/609 |
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