Antigen Presenting Properties of a Myeloid Dendritic-Like Cell in Murine Spleen.
This paper distinguishes a rare subset of myeloid dendritic-like cells found in mouse spleen from conventional (c) dendritic cells (DC) in terms of phenotype, function and gene expression. These cells are tentatively named "L-DC" since they resemble dendritic-like cells produced in longter...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5031434?pdf=render |
| _version_ | 1818509375271075840 |
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| author | Ying-Ying Hey Helen C O'Neill |
| author_facet | Ying-Ying Hey Helen C O'Neill |
| author_sort | Ying-Ying Hey |
| collection | DOAJ |
| description | This paper distinguishes a rare subset of myeloid dendritic-like cells found in mouse spleen from conventional (c) dendritic cells (DC) in terms of phenotype, function and gene expression. These cells are tentatively named "L-DC" since they resemble dendritic-like cells produced in longterm cultures of spleen. L-DC can be distinguished on the basis of their unique phenotype as CD11bhiCD11cloMHCII-CD43+Ly6C-Ly6G-Siglec-F- cells. They demonstrate similar ability as cDC to uptake and retain complex antigens like mannan via mannose receptors, but much lower ability to endocytose and retain soluble antigen. While L-DC differ from cDC by their inability to activate CD4+ T cells, they are capable of antigen cross-presentation for activation of CD8+ T cells, although less effectively so than the cDC subsets. In terms of gene expression, CD8- cDC and CD8+ cDC are quite distinct from L-DC. CD8+ cDC are distinguishable from the other two subsets by expression of CD24a, Clec9a, Xcr1 and Tlr11, while CD8- cDC are distinguished by expression of Ccnd1 and H-2Eb2. L-DC are distinct from the two cDC subsets through upregulated expression of Clec4a3, Emr4, Itgam, Csf1r and CD300ld. The L-DC gene profile is quite distinct from that of cDC, confirming a myeloid cell type with distinct antigen presenting properties. |
| first_indexed | 2024-12-10T22:44:41Z |
| format | Article |
| id | doaj.art-ce0550c8b52542c39633eea4e7e30f8b |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T22:44:41Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-ce0550c8b52542c39633eea4e7e30f8b2022-12-22T01:30:37ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01119e016235810.1371/journal.pone.0162358Antigen Presenting Properties of a Myeloid Dendritic-Like Cell in Murine Spleen.Ying-Ying HeyHelen C O'NeillThis paper distinguishes a rare subset of myeloid dendritic-like cells found in mouse spleen from conventional (c) dendritic cells (DC) in terms of phenotype, function and gene expression. These cells are tentatively named "L-DC" since they resemble dendritic-like cells produced in longterm cultures of spleen. L-DC can be distinguished on the basis of their unique phenotype as CD11bhiCD11cloMHCII-CD43+Ly6C-Ly6G-Siglec-F- cells. They demonstrate similar ability as cDC to uptake and retain complex antigens like mannan via mannose receptors, but much lower ability to endocytose and retain soluble antigen. While L-DC differ from cDC by their inability to activate CD4+ T cells, they are capable of antigen cross-presentation for activation of CD8+ T cells, although less effectively so than the cDC subsets. In terms of gene expression, CD8- cDC and CD8+ cDC are quite distinct from L-DC. CD8+ cDC are distinguishable from the other two subsets by expression of CD24a, Clec9a, Xcr1 and Tlr11, while CD8- cDC are distinguished by expression of Ccnd1 and H-2Eb2. L-DC are distinct from the two cDC subsets through upregulated expression of Clec4a3, Emr4, Itgam, Csf1r and CD300ld. The L-DC gene profile is quite distinct from that of cDC, confirming a myeloid cell type with distinct antigen presenting properties.http://europepmc.org/articles/PMC5031434?pdf=render |
| spellingShingle | Ying-Ying Hey Helen C O'Neill Antigen Presenting Properties of a Myeloid Dendritic-Like Cell in Murine Spleen. PLoS ONE |
| title | Antigen Presenting Properties of a Myeloid Dendritic-Like Cell in Murine Spleen. |
| title_full | Antigen Presenting Properties of a Myeloid Dendritic-Like Cell in Murine Spleen. |
| title_fullStr | Antigen Presenting Properties of a Myeloid Dendritic-Like Cell in Murine Spleen. |
| title_full_unstemmed | Antigen Presenting Properties of a Myeloid Dendritic-Like Cell in Murine Spleen. |
| title_short | Antigen Presenting Properties of a Myeloid Dendritic-Like Cell in Murine Spleen. |
| title_sort | antigen presenting properties of a myeloid dendritic like cell in murine spleen |
| url | http://europepmc.org/articles/PMC5031434?pdf=render |
| work_keys_str_mv | AT yingyinghey antigenpresentingpropertiesofamyeloiddendriticlikecellinmurinespleen AT helenconeill antigenpresentingpropertiesofamyeloiddendriticlikecellinmurinespleen |