Ameliorative Effect of <i>Posidonia oceanica</i> on High Glucose-Related Stress in Human Hepatoma HepG2 Cells
Metabolic disorders characterized by elevated blood glucose levels are a recognized risk factor for hepatocellular carcinoma (HCC). Lipid dysregulation is critically involved in the HCC progression, regulating energy storage, metabolism, and cell signaling. There is a clear link between de novo lipo...
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| Format: | Article |
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MDPI AG
2023-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/6/5203 |
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| author | Marzia Vasarri Emanuela Barletta Maria Stio Maria Camilla Bergonzi Andrea Galli Donatella Degl’Innocenti |
| author_facet | Marzia Vasarri Emanuela Barletta Maria Stio Maria Camilla Bergonzi Andrea Galli Donatella Degl’Innocenti |
| author_sort | Marzia Vasarri |
| collection | DOAJ |
| description | Metabolic disorders characterized by elevated blood glucose levels are a recognized risk factor for hepatocellular carcinoma (HCC). Lipid dysregulation is critically involved in the HCC progression, regulating energy storage, metabolism, and cell signaling. There is a clear link between de novo lipogenesis in the liver and activation of the NF-κB pathway, which is involved in cancer metastasis via regulation of metalloproteinases MMP-2/9. As conventional therapies for HCC reach their limits, new effective and safe drugs need to be found for the prevention and/or adjuvant therapy of HCC. The marine plant <i>Posidonia oceanica</i> (L.) Delile is endemic to the Mediterranean and has traditionally been used to treat diabetes and other health disorders. The phenol-rich leaf extract of <i>Posidonia oceanica</i> (POE) is known to have cell-safe bioactivities. Here, high glucose (HG) conditions were used to study lipid accumulation and fatty acid synthase (FASN) expression in human HepG2 hepatoma cells using Oil Red O and Western blot assays. Under HG conditions, the activation status of MAPKs/NF-κB axis and MMP-2/9 activity were determined by Western blot and gelatin zymography assays. The potential ameliorative role of POE against HG-related stress in HepG2 cells was then investigated. POE reduced lipid accumulation and FASN expression with an impact on de novo lipogenesis. Moreover, POE inhibited the MAPKs/NF-κB axis and, consequently, MMP-2/9 activity. Overall, these results suggest that <i>P. oceanica</i> may be a potential weapon in the HCC additional treatment. |
| first_indexed | 2024-03-11T06:27:28Z |
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| id | doaj.art-ce08a65813254e8b8bd2c4c0510b32f5 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T06:27:28Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-ce08a65813254e8b8bd2c4c0510b32f52023-11-17T11:30:34ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-03-01246520310.3390/ijms24065203Ameliorative Effect of <i>Posidonia oceanica</i> on High Glucose-Related Stress in Human Hepatoma HepG2 CellsMarzia Vasarri0Emanuela Barletta1Maria Stio2Maria Camilla Bergonzi3Andrea Galli4Donatella Degl’Innocenti5Department of Experimental and Clinical Biomedical Sciences, University of Florence, Viale Morgagni 50, 50134 Florence, ItalyDepartment of Experimental and Clinical Biomedical Sciences, University of Florence, Viale Morgagni 50, 50134 Florence, ItalyDepartment of Experimental and Clinical Biomedical Sciences, University of Florence, Viale Morgagni 50, 50134 Florence, ItalyDepartment of Chemistry “Ugo Schiff”, University of Florence, Via Ugo Schiff 6, 50019 Sesto Fiorentino, Florence, ItalyDepartment of Experimental and Clinical Biomedical Sciences, University of Florence, Viale Morgagni 50, 50134 Florence, ItalyDepartment of Experimental and Clinical Biomedical Sciences, University of Florence, Viale Morgagni 50, 50134 Florence, ItalyMetabolic disorders characterized by elevated blood glucose levels are a recognized risk factor for hepatocellular carcinoma (HCC). Lipid dysregulation is critically involved in the HCC progression, regulating energy storage, metabolism, and cell signaling. There is a clear link between de novo lipogenesis in the liver and activation of the NF-κB pathway, which is involved in cancer metastasis via regulation of metalloproteinases MMP-2/9. As conventional therapies for HCC reach their limits, new effective and safe drugs need to be found for the prevention and/or adjuvant therapy of HCC. The marine plant <i>Posidonia oceanica</i> (L.) Delile is endemic to the Mediterranean and has traditionally been used to treat diabetes and other health disorders. The phenol-rich leaf extract of <i>Posidonia oceanica</i> (POE) is known to have cell-safe bioactivities. Here, high glucose (HG) conditions were used to study lipid accumulation and fatty acid synthase (FASN) expression in human HepG2 hepatoma cells using Oil Red O and Western blot assays. Under HG conditions, the activation status of MAPKs/NF-κB axis and MMP-2/9 activity were determined by Western blot and gelatin zymography assays. The potential ameliorative role of POE against HG-related stress in HepG2 cells was then investigated. POE reduced lipid accumulation and FASN expression with an impact on de novo lipogenesis. Moreover, POE inhibited the MAPKs/NF-κB axis and, consequently, MMP-2/9 activity. Overall, these results suggest that <i>P. oceanica</i> may be a potential weapon in the HCC additional treatment.https://www.mdpi.com/1422-0067/24/6/5203lipid accumulationhigh glucoseNF-κBMMP-2/9HepG2 cells<i>Posidonia oceanica</i> |
| spellingShingle | Marzia Vasarri Emanuela Barletta Maria Stio Maria Camilla Bergonzi Andrea Galli Donatella Degl’Innocenti Ameliorative Effect of <i>Posidonia oceanica</i> on High Glucose-Related Stress in Human Hepatoma HepG2 Cells International Journal of Molecular Sciences lipid accumulation high glucose NF-κB MMP-2/9 HepG2 cells <i>Posidonia oceanica</i> |
| title | Ameliorative Effect of <i>Posidonia oceanica</i> on High Glucose-Related Stress in Human Hepatoma HepG2 Cells |
| title_full | Ameliorative Effect of <i>Posidonia oceanica</i> on High Glucose-Related Stress in Human Hepatoma HepG2 Cells |
| title_fullStr | Ameliorative Effect of <i>Posidonia oceanica</i> on High Glucose-Related Stress in Human Hepatoma HepG2 Cells |
| title_full_unstemmed | Ameliorative Effect of <i>Posidonia oceanica</i> on High Glucose-Related Stress in Human Hepatoma HepG2 Cells |
| title_short | Ameliorative Effect of <i>Posidonia oceanica</i> on High Glucose-Related Stress in Human Hepatoma HepG2 Cells |
| title_sort | ameliorative effect of i posidonia oceanica i on high glucose related stress in human hepatoma hepg2 cells |
| topic | lipid accumulation high glucose NF-κB MMP-2/9 HepG2 cells <i>Posidonia oceanica</i> |
| url | https://www.mdpi.com/1422-0067/24/6/5203 |
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