Molecular Characteristics of Pseudomonas syringae pv. actinidiae Strains Isolated in Korea and a Multiplex PCR Assay for Haplotype Differentiation
The molecular features of Pseudomonas syringae pv. actinidiae strains isolated in Korea were compared with strains isolated in Japan and Italy. Sequencing of eight P. syringae pv. actinidiae and three P. syringae pv. theae strains revealed a total of 44 single nucleotide polymorphisms across 4,818 b...
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| Format: | Article |
| Language: | English |
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Hanrimwon Publishing Company
2014-03-01
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| Series: | The Plant Pathology Journal |
| Subjects: | |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4174834/ |
| _version_ | 1828451034129760256 |
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| author | Hyun Seok Koh Gyoung Hee Kim Young Sun Lee Young Jin Koh Jae Sung Jung |
| author_facet | Hyun Seok Koh Gyoung Hee Kim Young Sun Lee Young Jin Koh Jae Sung Jung |
| author_sort | Hyun Seok Koh |
| collection | DOAJ |
| description | The molecular features of Pseudomonas syringae pv. actinidiae strains isolated in Korea were compared with strains isolated in Japan and Italy. Sequencing of eight P. syringae pv. actinidiae and three P. syringae pv. theae strains revealed a total of 44 single nucleotide polymorphisms across 4,818 bp of the concatenated alignment of nine genes. A multiplex PCR assay was developed for the detection of P. syringae pv. actinidiae and for the specific detection of recent haplotype strains other than strains isolated since the 1980s in Korea. The primer pair, designated as TacF and TacR, specifically amplified a 545-bp fragment with the genomic DNA of new haplotype of P. syringae pv. actinidiae strains. A multiplex PCR conducted with the TacF/TacR primer pair and the universal primer pair for all P. syringae pv. actinidiae strains can be simultaneously applied for the detection of P. syringae pv. actinidiae and for the differentiation of new haplotype strains. |
| first_indexed | 2024-12-10T23:31:10Z |
| format | Article |
| id | doaj.art-ce0b776d72c3494fb48f416251859698 |
| institution | Directory Open Access Journal |
| issn | 1598-2254 |
| language | English |
| last_indexed | 2024-12-10T23:31:10Z |
| publishDate | 2014-03-01 |
| publisher | Hanrimwon Publishing Company |
| record_format | Article |
| series | The Plant Pathology Journal |
| spelling | doaj.art-ce0b776d72c3494fb48f4162518596982022-12-22T01:29:23ZengHanrimwon Publishing CompanyThe Plant Pathology Journal1598-22542014-03-013019610110.5423/PPJ.NT.09.2013.0095PPJ.NT.09.2013.0095Molecular Characteristics of Pseudomonas syringae pv. actinidiae Strains Isolated in Korea and a Multiplex PCR Assay for Haplotype DifferentiationHyun Seok Koh0Gyoung Hee Kim1Young Sun Lee2Young Jin Koh3Jae Sung Jung4Department of Biology, Sunchon National University, Suncheon 540-950, KoreaDepartment of Plant Medicine, Sunchon National University, Suncheon 540-950, KoreaDepartment of Biology, Sunchon National University, Suncheon 540-950, KoreaDepartment of Plant Medicine, Sunchon National University, Suncheon 540-950, KoreaDepartment of Biology, Sunchon National University, Suncheon 540-950, KoreaThe molecular features of Pseudomonas syringae pv. actinidiae strains isolated in Korea were compared with strains isolated in Japan and Italy. Sequencing of eight P. syringae pv. actinidiae and three P. syringae pv. theae strains revealed a total of 44 single nucleotide polymorphisms across 4,818 bp of the concatenated alignment of nine genes. A multiplex PCR assay was developed for the detection of P. syringae pv. actinidiae and for the specific detection of recent haplotype strains other than strains isolated since the 1980s in Korea. The primer pair, designated as TacF and TacR, specifically amplified a 545-bp fragment with the genomic DNA of new haplotype of P. syringae pv. actinidiae strains. A multiplex PCR conducted with the TacF/TacR primer pair and the universal primer pair for all P. syringae pv. actinidiae strains can be simultaneously applied for the detection of P. syringae pv. actinidiae and for the differentiation of new haplotype strains.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4174834/bacterial cankerkiwifruitmultiplex PCRPCR detectionPseudomonas syringae pv. actinidiae |
| spellingShingle | Hyun Seok Koh Gyoung Hee Kim Young Sun Lee Young Jin Koh Jae Sung Jung Molecular Characteristics of Pseudomonas syringae pv. actinidiae Strains Isolated in Korea and a Multiplex PCR Assay for Haplotype Differentiation The Plant Pathology Journal bacterial canker kiwifruit multiplex PCR PCR detection Pseudomonas syringae pv. actinidiae |
| title | Molecular Characteristics of Pseudomonas syringae pv. actinidiae Strains Isolated in Korea and a Multiplex PCR Assay for Haplotype Differentiation |
| title_full | Molecular Characteristics of Pseudomonas syringae pv. actinidiae Strains Isolated in Korea and a Multiplex PCR Assay for Haplotype Differentiation |
| title_fullStr | Molecular Characteristics of Pseudomonas syringae pv. actinidiae Strains Isolated in Korea and a Multiplex PCR Assay for Haplotype Differentiation |
| title_full_unstemmed | Molecular Characteristics of Pseudomonas syringae pv. actinidiae Strains Isolated in Korea and a Multiplex PCR Assay for Haplotype Differentiation |
| title_short | Molecular Characteristics of Pseudomonas syringae pv. actinidiae Strains Isolated in Korea and a Multiplex PCR Assay for Haplotype Differentiation |
| title_sort | molecular characteristics of pseudomonas syringae pv actinidiae strains isolated in korea and a multiplex pcr assay for haplotype differentiation |
| topic | bacterial canker kiwifruit multiplex PCR PCR detection Pseudomonas syringae pv. actinidiae |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4174834/ |
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