A Microsurgical Arteriovenous Malformation Model on Saphenous Vessels in the Rat
Arteriovenous malformation (AVM) is an anomaly of blood vessel formation. Numerous models have been established to understand the nature of AVM. These models have limitations in terms of the diameter of the vessels used and the impact on the circulatory system. Our goal was to establish an AVM model...
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| Language: | English |
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MDPI AG
2023-11-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/11/11/2970 |
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| author | Mohammad Walid Al-Smadi Laszlo Adam Fazekas Siran Aslan Brigitta Bernat Anas Beqain Mustafa Qais Muhsin Al-Khafaji Daniel Priksz Brigitta Orlik Norbert Nemeth |
| author_facet | Mohammad Walid Al-Smadi Laszlo Adam Fazekas Siran Aslan Brigitta Bernat Anas Beqain Mustafa Qais Muhsin Al-Khafaji Daniel Priksz Brigitta Orlik Norbert Nemeth |
| author_sort | Mohammad Walid Al-Smadi |
| collection | DOAJ |
| description | Arteriovenous malformation (AVM) is an anomaly of blood vessel formation. Numerous models have been established to understand the nature of AVM. These models have limitations in terms of the diameter of the vessels used and the impact on the circulatory system. Our goal was to establish an AVM model that does not cause prompt and significant hemodynamic and cardiac alterations but is feasible for follow-up of the AVM’s progression. Sixteen female rats were randomly divided into sham-operated and AVM groups. In the AVM group, the saphenous vein and artery were interconnected using microsurgical techniques. The animals were followed up for 12 weeks. Anastomosis patency and the structural and hemodynamic changes of the heart were monitored. The hearts and vessels were histologically analyzed. During the follow-up period, shunts remained unobstructed. Systolic, diastolic, mean arterial pressure, and heart rate values slightly and non-significantly decreased in the AVM group. Echocardiogram results indicated minor systolic function impact, with slight and insignificant changes in aortic pressure and blood velocity, and minimal left ventricular wall enlargement. The small-caliber saphenous AVM model does not cause acute hemodynamic changes. Moderate but progressive alterations and venous dilatation confirmed AVM-like features. The model seems to be suitable for studying further the progression, enlargement, or destabilization of AVM. |
| first_indexed | 2024-03-09T17:00:04Z |
| format | Article |
| id | doaj.art-ce0dc1b3b8c04a11b013e86160d8a430 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-09T17:00:04Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-ce0dc1b3b8c04a11b013e86160d8a4302023-11-24T14:30:59ZengMDPI AGBiomedicines2227-90592023-11-011111297010.3390/biomedicines11112970A Microsurgical Arteriovenous Malformation Model on Saphenous Vessels in the RatMohammad Walid Al-Smadi0Laszlo Adam Fazekas1Siran Aslan2Brigitta Bernat3Anas Beqain4Mustafa Qais Muhsin Al-Khafaji5Daniel Priksz6Brigitta Orlik7Norbert Nemeth8Department of Operative Techniques and Surgical Research, Faculty of Medicine, University of Debrecen, Moricz Zsigmond ut 22, 4032 Debrecen, HungaryDepartment of Operative Techniques and Surgical Research, Faculty of Medicine, University of Debrecen, Moricz Zsigmond ut 22, 4032 Debrecen, HungaryDepartment of Operative Techniques and Surgical Research, Faculty of Medicine, University of Debrecen, Moricz Zsigmond ut 22, 4032 Debrecen, HungaryDepartment of Pharmacology and Pharmacotherapy, Faculty of Medicine, University of Debrecen, Nagyerdei krt. 98, 4032 Debrecen, HungaryDepartment of Operative Techniques and Surgical Research, Faculty of Medicine, University of Debrecen, Moricz Zsigmond ut 22, 4032 Debrecen, HungaryDepartment of Operative Techniques and Surgical Research, Faculty of Medicine, University of Debrecen, Moricz Zsigmond ut 22, 4032 Debrecen, HungaryDepartment of Pharmacology and Pharmacotherapy, Faculty of Medicine, University of Debrecen, Nagyerdei krt. 98, 4032 Debrecen, HungaryDepartment of Pathology, Faculty of Medicine, University of Debrecen, Nagyerdei krt. 98, 4032 Debrecen, HungaryDepartment of Operative Techniques and Surgical Research, Faculty of Medicine, University of Debrecen, Moricz Zsigmond ut 22, 4032 Debrecen, HungaryArteriovenous malformation (AVM) is an anomaly of blood vessel formation. Numerous models have been established to understand the nature of AVM. These models have limitations in terms of the diameter of the vessels used and the impact on the circulatory system. Our goal was to establish an AVM model that does not cause prompt and significant hemodynamic and cardiac alterations but is feasible for follow-up of the AVM’s progression. Sixteen female rats were randomly divided into sham-operated and AVM groups. In the AVM group, the saphenous vein and artery were interconnected using microsurgical techniques. The animals were followed up for 12 weeks. Anastomosis patency and the structural and hemodynamic changes of the heart were monitored. The hearts and vessels were histologically analyzed. During the follow-up period, shunts remained unobstructed. Systolic, diastolic, mean arterial pressure, and heart rate values slightly and non-significantly decreased in the AVM group. Echocardiogram results indicated minor systolic function impact, with slight and insignificant changes in aortic pressure and blood velocity, and minimal left ventricular wall enlargement. The small-caliber saphenous AVM model does not cause acute hemodynamic changes. Moderate but progressive alterations and venous dilatation confirmed AVM-like features. The model seems to be suitable for studying further the progression, enlargement, or destabilization of AVM.https://www.mdpi.com/2227-9059/11/11/2970arteriovenous malformationarteriovenous shuntmicrosurgical modelvascular remodellinghemodynamics |
| spellingShingle | Mohammad Walid Al-Smadi Laszlo Adam Fazekas Siran Aslan Brigitta Bernat Anas Beqain Mustafa Qais Muhsin Al-Khafaji Daniel Priksz Brigitta Orlik Norbert Nemeth A Microsurgical Arteriovenous Malformation Model on Saphenous Vessels in the Rat Biomedicines arteriovenous malformation arteriovenous shunt microsurgical model vascular remodelling hemodynamics |
| title | A Microsurgical Arteriovenous Malformation Model on Saphenous Vessels in the Rat |
| title_full | A Microsurgical Arteriovenous Malformation Model on Saphenous Vessels in the Rat |
| title_fullStr | A Microsurgical Arteriovenous Malformation Model on Saphenous Vessels in the Rat |
| title_full_unstemmed | A Microsurgical Arteriovenous Malformation Model on Saphenous Vessels in the Rat |
| title_short | A Microsurgical Arteriovenous Malformation Model on Saphenous Vessels in the Rat |
| title_sort | microsurgical arteriovenous malformation model on saphenous vessels in the rat |
| topic | arteriovenous malformation arteriovenous shunt microsurgical model vascular remodelling hemodynamics |
| url | https://www.mdpi.com/2227-9059/11/11/2970 |
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