Evaluation of delphinidin as a storage medium for avulsed teeth
Abstract Background Delphinidin (DP), an anthocyanidin found in blueberries, has antioxidant and anti-inflammatory effects. This study aimed to investigate the efficacy of DP as a storage medium for avulsed teeth. Methods Human periodontal ligament cells were cultured and exposed to DP solution (10,...
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| Format: | Article |
| Language: | English |
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BMC
2023-01-01
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| Series: | BMC Oral Health |
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| Online Access: | https://doi.org/10.1186/s12903-023-02713-9 |
| _version_ | 1797952374488170496 |
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| author | Ok Hyung Nam Sang Tae Ro Hyeon-Woo Lee Jaeki Jeong Yong Kwon Chae Ko Eun Lee Sung Chul Choi Sang Wook Kang |
| author_facet | Ok Hyung Nam Sang Tae Ro Hyeon-Woo Lee Jaeki Jeong Yong Kwon Chae Ko Eun Lee Sung Chul Choi Sang Wook Kang |
| author_sort | Ok Hyung Nam |
| collection | DOAJ |
| description | Abstract Background Delphinidin (DP), an anthocyanidin found in blueberries, has antioxidant and anti-inflammatory effects. This study aimed to investigate the efficacy of DP as a storage medium for avulsed teeth. Methods Human periodontal ligament cells were cultured and exposed to DP solution (10, 50, and 100 μM), Dulbecco’s modified Eagle’s medium, Hank’s balanced salt solution and tap water. Cell counting kit-8 assays were performed after 0.5, 1, 6, and 24 h to measure the cell viability. Nitric oxide assays and gelatin zymography were performed to evaluate the anti-inflammatory effects of DP. Reverse transcription-polymerase chain reaction was used to determine the expression levels of inflammatory cytokines. Results The viability of periodontal ligament cells was greatest at 100 μM DP. At 1 h, 100 μM DP decreased nitric oxide synthesis (p < .0167). Matrix metallopeptidase-9 activity was inhibited by DP in a dose-dependent manner (p < .0167). Moreover, treatment with 100 μM DP decreased the expression levels of tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-8 in periodontal ligament cells (p < .0167). Conclusions Within the limits of this study, DP preserved the viability and suppressed the inflammatory response of periodontal ligament cells. These findings suggest that DP could be promising for preservation of avulsed teeth. |
| first_indexed | 2024-04-10T22:45:20Z |
| format | Article |
| id | doaj.art-ce0e854fd29d4eeab9de7a924e84f54f |
| institution | Directory Open Access Journal |
| issn | 1472-6831 |
| language | English |
| last_indexed | 2024-04-10T22:45:20Z |
| publishDate | 2023-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Oral Health |
| spelling | doaj.art-ce0e854fd29d4eeab9de7a924e84f54f2023-01-15T12:23:03ZengBMCBMC Oral Health1472-68312023-01-012311710.1186/s12903-023-02713-9Evaluation of delphinidin as a storage medium for avulsed teethOk Hyung Nam0Sang Tae Ro1Hyeon-Woo Lee2Jaeki Jeong3Yong Kwon Chae4Ko Eun Lee5Sung Chul Choi6Sang Wook Kang7Department of Pediatric Dentistry, School of Dentistry, Kyung Hee UniversityDepartment of Dentistry, Graduate School, Kyung Hee UniversityDepartment of Pharmacology, School of Dentistry, Kyung Hee UniversityDepartment of Dentistry, Graduate School, Kyung Hee UniversityDepartment of Pediatric Dentistry, Kyung Hee University College of Dentistry, Kyung Hee University Medical CenterDepartment of Pediatric Dentistry, Kyung Hee University College of Dentistry, Kyung Hee University Medical CenterDepartment of Pediatric Dentistry, School of Dentistry, Kyung Hee UniversityDepartment of Oral and Maxillofacial Pathology, School of Dentistry, Kyung Hee UniversityAbstract Background Delphinidin (DP), an anthocyanidin found in blueberries, has antioxidant and anti-inflammatory effects. This study aimed to investigate the efficacy of DP as a storage medium for avulsed teeth. Methods Human periodontal ligament cells were cultured and exposed to DP solution (10, 50, and 100 μM), Dulbecco’s modified Eagle’s medium, Hank’s balanced salt solution and tap water. Cell counting kit-8 assays were performed after 0.5, 1, 6, and 24 h to measure the cell viability. Nitric oxide assays and gelatin zymography were performed to evaluate the anti-inflammatory effects of DP. Reverse transcription-polymerase chain reaction was used to determine the expression levels of inflammatory cytokines. Results The viability of periodontal ligament cells was greatest at 100 μM DP. At 1 h, 100 μM DP decreased nitric oxide synthesis (p < .0167). Matrix metallopeptidase-9 activity was inhibited by DP in a dose-dependent manner (p < .0167). Moreover, treatment with 100 μM DP decreased the expression levels of tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-8 in periodontal ligament cells (p < .0167). Conclusions Within the limits of this study, DP preserved the viability and suppressed the inflammatory response of periodontal ligament cells. These findings suggest that DP could be promising for preservation of avulsed teeth.https://doi.org/10.1186/s12903-023-02713-9Avulsed toothPeriodontal ligament cellStorage mediumTooth replantation |
| spellingShingle | Ok Hyung Nam Sang Tae Ro Hyeon-Woo Lee Jaeki Jeong Yong Kwon Chae Ko Eun Lee Sung Chul Choi Sang Wook Kang Evaluation of delphinidin as a storage medium for avulsed teeth BMC Oral Health Avulsed tooth Periodontal ligament cell Storage medium Tooth replantation |
| title | Evaluation of delphinidin as a storage medium for avulsed teeth |
| title_full | Evaluation of delphinidin as a storage medium for avulsed teeth |
| title_fullStr | Evaluation of delphinidin as a storage medium for avulsed teeth |
| title_full_unstemmed | Evaluation of delphinidin as a storage medium for avulsed teeth |
| title_short | Evaluation of delphinidin as a storage medium for avulsed teeth |
| title_sort | evaluation of delphinidin as a storage medium for avulsed teeth |
| topic | Avulsed tooth Periodontal ligament cell Storage medium Tooth replantation |
| url | https://doi.org/10.1186/s12903-023-02713-9 |
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