Clinical and Mycological Characterization of Chronic and Recurrent Dermatophytes using Various Staining and Microscopic Methods
Dermatophytosis is a prevalent infection in tropical and subtropical countries, including India. This study aims to investigate the epidemiology and clinical aspects of chronic and recurrent dermatophytosis, identify the clinical isolates, and assess the effectiveness of different microscopy and sta...
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| Format: | Article |
| Language: | English |
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Journal of Pure and Applied Microbiology
2023-12-01
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| Series: | Journal of Pure and Applied Microbiology |
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| Online Access: | https://microbiologyjournal.org/clinical-and-mycological-characterization-of-chronic-and-recurrent-dermatophytes-using-various-staining-and-microscopic-methods/ |
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| author | Nikhitha Amin M. Manjunath Shenoy Vidya Pai |
| author_facet | Nikhitha Amin M. Manjunath Shenoy Vidya Pai |
| author_sort | Nikhitha Amin |
| collection | DOAJ |
| description | Dermatophytosis is a prevalent infection in tropical and subtropical countries, including India. This study aims to investigate the epidemiology and clinical aspects of chronic and recurrent dermatophytosis, identify the clinical isolates, and assess the effectiveness of different microscopy and staining methods for diagnosis. The objective of the study is to study the epidemiology and clinical aspects of chronic and recurrent dermatophytosis and to identify the clinical isolates. Attempts to improve the diagnostic outcome by implementing different microscopy and staining methods have also been performed. Adult patients with chronic and recurrent cases and positive direct microscopy were included, and clinical details were recorded. The mycological culture was performed. Fifteen isolates were selected randomly and subjected to 0.9% NaCl, Chicago sky blue staining, Calcofluor white staining, and Congo red staining in addition to the standard lactophenol cotton blue (LPCB) preparation. Among the 178 patients in the study, females (56.7%) and patients aged 25-45 (50%) were more frequently affected. Tinea corporis was the most common clinical type (89.32%). Recurrent cases (56.1%) were more prevalent than chronic cases (43.9%). Culture positivity was seen in 60.1%, with Trichophyton mentagrophytes being the most common isolate (86%). Among the staining methods used on 15 selected isolates, Lactophenol Cotton Blue (LPCB) was scored as the most effective, scoring 2.6 out of 3. The study revealed several clinical and epidemiological findings related to dermatophytosis in India, including a high degree of communicability, inadvertent therapies including topical steroid misuse, and a persistent nature requiring an extended duration of therapy. Trichophyton mentagrophytes was the most frequently isolated pathogen. Of the staining methods evaluated, Lactophenol Cotton Blue (LPCB) was found to be the most effective. The findings suggest a need for continued research into effective treatments and diagnostic methods for dermatophytosis in India. |
| first_indexed | 2024-03-08T22:51:02Z |
| format | Article |
| id | doaj.art-ce0fe8bc5c084469ad00b68751a57c8f |
| institution | Directory Open Access Journal |
| issn | 0973-7510 2581-690X |
| language | English |
| last_indexed | 2024-03-08T22:51:02Z |
| publishDate | 2023-12-01 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | Article |
| series | Journal of Pure and Applied Microbiology |
| spelling | doaj.art-ce0fe8bc5c084469ad00b68751a57c8f2023-12-16T14:12:31ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2023-12-0117425982608https://doi.org/10.22207/JPAM.17.4.59Clinical and Mycological Characterization of Chronic and Recurrent Dermatophytes using Various Staining and Microscopic MethodsNikhitha AminM. Manjunath Shenoyhttps://orcid.org/0000-0002-2985-9623Vidya Paihttps://orcid.org/0000-0002-4021-8341Dermatophytosis is a prevalent infection in tropical and subtropical countries, including India. This study aims to investigate the epidemiology and clinical aspects of chronic and recurrent dermatophytosis, identify the clinical isolates, and assess the effectiveness of different microscopy and staining methods for diagnosis. The objective of the study is to study the epidemiology and clinical aspects of chronic and recurrent dermatophytosis and to identify the clinical isolates. Attempts to improve the diagnostic outcome by implementing different microscopy and staining methods have also been performed. Adult patients with chronic and recurrent cases and positive direct microscopy were included, and clinical details were recorded. The mycological culture was performed. Fifteen isolates were selected randomly and subjected to 0.9% NaCl, Chicago sky blue staining, Calcofluor white staining, and Congo red staining in addition to the standard lactophenol cotton blue (LPCB) preparation. Among the 178 patients in the study, females (56.7%) and patients aged 25-45 (50%) were more frequently affected. Tinea corporis was the most common clinical type (89.32%). Recurrent cases (56.1%) were more prevalent than chronic cases (43.9%). Culture positivity was seen in 60.1%, with Trichophyton mentagrophytes being the most common isolate (86%). Among the staining methods used on 15 selected isolates, Lactophenol Cotton Blue (LPCB) was scored as the most effective, scoring 2.6 out of 3. The study revealed several clinical and epidemiological findings related to dermatophytosis in India, including a high degree of communicability, inadvertent therapies including topical steroid misuse, and a persistent nature requiring an extended duration of therapy. Trichophyton mentagrophytes was the most frequently isolated pathogen. Of the staining methods evaluated, Lactophenol Cotton Blue (LPCB) was found to be the most effective. The findings suggest a need for continued research into effective treatments and diagnostic methods for dermatophytosis in India.https://microbiologyjournal.org/clinical-and-mycological-characterization-of-chronic-and-recurrent-dermatophytes-using-various-staining-and-microscopic-methods/chronic dermatophytosisrecurrent dermatophytosismycological diagnosislactophenol cotton bluecalcofluor whitecongo red |
| spellingShingle | Nikhitha Amin M. Manjunath Shenoy Vidya Pai Clinical and Mycological Characterization of Chronic and Recurrent Dermatophytes using Various Staining and Microscopic Methods Journal of Pure and Applied Microbiology chronic dermatophytosis recurrent dermatophytosis mycological diagnosis lactophenol cotton blue calcofluor white congo red |
| title | Clinical and Mycological Characterization of Chronic and Recurrent Dermatophytes using Various Staining and Microscopic Methods |
| title_full | Clinical and Mycological Characterization of Chronic and Recurrent Dermatophytes using Various Staining and Microscopic Methods |
| title_fullStr | Clinical and Mycological Characterization of Chronic and Recurrent Dermatophytes using Various Staining and Microscopic Methods |
| title_full_unstemmed | Clinical and Mycological Characterization of Chronic and Recurrent Dermatophytes using Various Staining and Microscopic Methods |
| title_short | Clinical and Mycological Characterization of Chronic and Recurrent Dermatophytes using Various Staining and Microscopic Methods |
| title_sort | clinical and mycological characterization of chronic and recurrent dermatophytes using various staining and microscopic methods |
| topic | chronic dermatophytosis recurrent dermatophytosis mycological diagnosis lactophenol cotton blue calcofluor white congo red |
| url | https://microbiologyjournal.org/clinical-and-mycological-characterization-of-chronic-and-recurrent-dermatophytes-using-various-staining-and-microscopic-methods/ |
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