Impact of <i>Escherichia coli</i> Outer Membrane Vesicles on Sperm Function
Reproductive tract infections account for approximately 15% of male infertility cases. <i>Escherichia coli</i> (<i>E. coli</i>) represents the most frequently isolated bacterial strain in the semen of infertile men. All Gram-negative bacteria constitutively produce outer memb...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-07-01
|
| Series: | Pathogens |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-0817/11/7/782 |
| _version_ | 1827618750188224512 |
|---|---|
| author | Veronica Folliero Marianna Santonastaso Federica Dell’Annunziata Pasquale De Franciscis Giovanni Boccia Nicola Colacurci Anna De Filippis Massimiliano Galdiero Gianluigi Franci |
| author_facet | Veronica Folliero Marianna Santonastaso Federica Dell’Annunziata Pasquale De Franciscis Giovanni Boccia Nicola Colacurci Anna De Filippis Massimiliano Galdiero Gianluigi Franci |
| author_sort | Veronica Folliero |
| collection | DOAJ |
| description | Reproductive tract infections account for approximately 15% of male infertility cases. <i>Escherichia coli</i> (<i>E. coli</i>) represents the most frequently isolated bacterial strain in the semen of infertile men. All Gram-negative bacteria constitutively produce outer membrane vesicles (OMVs). The present study proved, for the first time, the involvement of OMVs in human sperm function. <i>E. coli</i> OMVs were isolated by ultracentrifugation and characterized via sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE), transmission electron microscopy (TEM) and dynamic light scattering (DLS) analysis. Human sperm was exposed to OMVs (8 µg/mL) for different times (30, 45, 60 and 90 min). The vitality, motility, morphology, ROS level and DNA fragmentation of spermatozoa were evaluated. OMVs reduced the progressive motility and increased the immobile spermatozoa amount after 30 min of treatment. In addition, a significant increase in the percentage of intracellular ROS and sperm DNA fragmentation was recorded for each vesicular exposure time. These preliminary findings prove that OMVs contribute to altering human sperm function via two mechanisms: (i) impaired motility and (ii) DNA fragmentation. |
| first_indexed | 2024-03-09T10:13:51Z |
| format | Article |
| id | doaj.art-ce10d78d4ed9464da3ab46920f5b0e60 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T10:13:51Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-ce10d78d4ed9464da3ab46920f5b0e602023-12-01T22:32:46ZengMDPI AGPathogens2076-08172022-07-0111778210.3390/pathogens11070782Impact of <i>Escherichia coli</i> Outer Membrane Vesicles on Sperm FunctionVeronica Folliero0Marianna Santonastaso1Federica Dell’Annunziata2Pasquale De Franciscis3Giovanni Boccia4Nicola Colacurci5Anna De Filippis6Massimiliano Galdiero7Gianluigi Franci8Department of Experimental Medicine, University of Campania Luigi Vanvitelli, 80138 Naples, ItalyDepartment of Woman, Child and General and Special Surgery, University of Campania Luigi Vanvitelli, 80138 Naples, ItalyDepartment of Experimental Medicine, University of Campania Luigi Vanvitelli, 80138 Naples, ItalyDepartment of Woman, Child and General and Special Surgery, University of Campania Luigi Vanvitelli, 80138 Naples, ItalyDepartment of Medicine, Surgery and Dentistry Scuola Medica Salernitana, University of Salerno, 84131 Baronissi, ItalyDepartment of Woman, Child and General and Special Surgery, University of Campania Luigi Vanvitelli, 80138 Naples, ItalyDepartment of Experimental Medicine, University of Campania Luigi Vanvitelli, 80138 Naples, ItalyDepartment of Experimental Medicine, University of Campania Luigi Vanvitelli, 80138 Naples, ItalyDepartment of Medicine, Surgery and Dentistry Scuola Medica Salernitana, University of Salerno, 84131 Baronissi, ItalyReproductive tract infections account for approximately 15% of male infertility cases. <i>Escherichia coli</i> (<i>E. coli</i>) represents the most frequently isolated bacterial strain in the semen of infertile men. All Gram-negative bacteria constitutively produce outer membrane vesicles (OMVs). The present study proved, for the first time, the involvement of OMVs in human sperm function. <i>E. coli</i> OMVs were isolated by ultracentrifugation and characterized via sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE), transmission electron microscopy (TEM) and dynamic light scattering (DLS) analysis. Human sperm was exposed to OMVs (8 µg/mL) for different times (30, 45, 60 and 90 min). The vitality, motility, morphology, ROS level and DNA fragmentation of spermatozoa were evaluated. OMVs reduced the progressive motility and increased the immobile spermatozoa amount after 30 min of treatment. In addition, a significant increase in the percentage of intracellular ROS and sperm DNA fragmentation was recorded for each vesicular exposure time. These preliminary findings prove that OMVs contribute to altering human sperm function via two mechanisms: (i) impaired motility and (ii) DNA fragmentation.https://www.mdpi.com/2076-0817/11/7/782outer membrane vesiclefertility<i>Escherichia coli</i> |
| spellingShingle | Veronica Folliero Marianna Santonastaso Federica Dell’Annunziata Pasquale De Franciscis Giovanni Boccia Nicola Colacurci Anna De Filippis Massimiliano Galdiero Gianluigi Franci Impact of <i>Escherichia coli</i> Outer Membrane Vesicles on Sperm Function Pathogens outer membrane vesicle fertility <i>Escherichia coli</i> |
| title | Impact of <i>Escherichia coli</i> Outer Membrane Vesicles on Sperm Function |
| title_full | Impact of <i>Escherichia coli</i> Outer Membrane Vesicles on Sperm Function |
| title_fullStr | Impact of <i>Escherichia coli</i> Outer Membrane Vesicles on Sperm Function |
| title_full_unstemmed | Impact of <i>Escherichia coli</i> Outer Membrane Vesicles on Sperm Function |
| title_short | Impact of <i>Escherichia coli</i> Outer Membrane Vesicles on Sperm Function |
| title_sort | impact of i escherichia coli i outer membrane vesicles on sperm function |
| topic | outer membrane vesicle fertility <i>Escherichia coli</i> |
| url | https://www.mdpi.com/2076-0817/11/7/782 |
| work_keys_str_mv | AT veronicafolliero impactofiescherichiacoliioutermembranevesiclesonspermfunction AT mariannasantonastaso impactofiescherichiacoliioutermembranevesiclesonspermfunction AT federicadellannunziata impactofiescherichiacoliioutermembranevesiclesonspermfunction AT pasqualedefranciscis impactofiescherichiacoliioutermembranevesiclesonspermfunction AT giovanniboccia impactofiescherichiacoliioutermembranevesiclesonspermfunction AT nicolacolacurci impactofiescherichiacoliioutermembranevesiclesonspermfunction AT annadefilippis impactofiescherichiacoliioutermembranevesiclesonspermfunction AT massimilianogaldiero impactofiescherichiacoliioutermembranevesiclesonspermfunction AT gianluigifranci impactofiescherichiacoliioutermembranevesiclesonspermfunction |