Methylation profile of bovine Oct4 gene coding region in relation to three germ layers
Previous studies have shown that octamer-binding transcription factor 4 (Oct4) plays a significant role in early embryonic development of mammalian animals, and different Oct4 expression levels induce multi-lineage differentiation which are regulated by DNA methylation. To explore the relationship b...
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| Format: | Article |
| Language: | English |
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Elsevier
2016-03-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S209531191661643X |
| _version_ | 1818900569253740544 |
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| author | Xin-yu ZHOU Liang-liang LIU Wen-chao JIA Chuan-ying PAN |
| author_facet | Xin-yu ZHOU Liang-liang LIU Wen-chao JIA Chuan-ying PAN |
| author_sort | Xin-yu ZHOU |
| collection | DOAJ |
| description | Previous studies have shown that octamer-binding transcription factor 4 (Oct4) plays a significant role in early embryonic development of mammalian animals, and different Oct4 expression levels induce multi-lineage differentiation which are regulated by DNA methylation. To explore the relationship between the methylation pattern of Oct4 gene exon 1 and embryonic development, in this work, five different tissues (heart, liver, lung, cerebrum and cerebellum) from three germ layers were chosen from low age (50–60 d) and advanced age (60–70 d) of fetal cattle and the differences between tissues or ages were analyzed, respectively. The result showed that the DNA methylation level of Oct4 gene exon 1 was significant different (P<0.01) between any two of three germ layers in low age (<60 d), but kept steady of advanced age (P>0.05) (>60 d), suggesting that 60-d post coital was an important boundary for embryonic development. In addition, in ectoderm (cerebrum and cerebellum), there was no significant methylation difference of Oct4 gene exon 1 between low age and advanced age (P>0.05), but the result of endoderm (liver and lung) and mesoderm (heart) were on the contrary (P<0.01), which indicated the development of ectoderm was earlier than endoderm and mesoderm. The methylation differences from the 3rd, 5th and 9th CpG-dinucleotide loci of Oct4 gene exon 1 were significantly different between each two of three germ layers (P<0.05), indicating that these three loci may have important influence on bovine embryonic development. This study showed that bovine germ layers differentiation was significantly related to the DNA methylation status of Oct4 gene exon 1. This work firstly identified the DNA methylation profile of bovine Oct4 gene exon 1 and its association with germ layers development in fetus and adult of cattle. Moreover, the work also provided epigenetic information for further studying bovine embryonic development and cellular reprogramming. |
| first_indexed | 2024-12-19T20:05:56Z |
| format | Article |
| id | doaj.art-ce12e068db4b4cb1b35ea93f6406eb26 |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-12-19T20:05:56Z |
| publishDate | 2016-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-ce12e068db4b4cb1b35ea93f6406eb262022-12-21T20:07:29ZengElsevierJournal of Integrative Agriculture2095-31192016-03-01153618628Methylation profile of bovine Oct4 gene coding region in relation to three germ layersXin-yu ZHOU0Liang-liang LIU1Wen-chao JIA2Chuan-ying PAN3College of Life Sciences, Northwest A&F University, Yangling 712100, P.R. ChinaSchool of Computer Science & Engineering, Jiangsu University of Science and Technology, Zhenjiang 212003, P.R. ChinaCollege of Animal Science and Technology, Northwest A&F University/Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling 712100, P.R. ChinaCollege of Life Sciences, Northwest A&F University, Yangling 712100, P.R. China; College of Animal Science and Technology, Northwest A&F University/Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling 712100, P.R. China; Correspondence PAN Chuan-ying, Tel: +86-29-87092102, Fax: +86-29-87092164Previous studies have shown that octamer-binding transcription factor 4 (Oct4) plays a significant role in early embryonic development of mammalian animals, and different Oct4 expression levels induce multi-lineage differentiation which are regulated by DNA methylation. To explore the relationship between the methylation pattern of Oct4 gene exon 1 and embryonic development, in this work, five different tissues (heart, liver, lung, cerebrum and cerebellum) from three germ layers were chosen from low age (50–60 d) and advanced age (60–70 d) of fetal cattle and the differences between tissues or ages were analyzed, respectively. The result showed that the DNA methylation level of Oct4 gene exon 1 was significant different (P<0.01) between any two of three germ layers in low age (<60 d), but kept steady of advanced age (P>0.05) (>60 d), suggesting that 60-d post coital was an important boundary for embryonic development. In addition, in ectoderm (cerebrum and cerebellum), there was no significant methylation difference of Oct4 gene exon 1 between low age and advanced age (P>0.05), but the result of endoderm (liver and lung) and mesoderm (heart) were on the contrary (P<0.01), which indicated the development of ectoderm was earlier than endoderm and mesoderm. The methylation differences from the 3rd, 5th and 9th CpG-dinucleotide loci of Oct4 gene exon 1 were significantly different between each two of three germ layers (P<0.05), indicating that these three loci may have important influence on bovine embryonic development. This study showed that bovine germ layers differentiation was significantly related to the DNA methylation status of Oct4 gene exon 1. This work firstly identified the DNA methylation profile of bovine Oct4 gene exon 1 and its association with germ layers development in fetus and adult of cattle. Moreover, the work also provided epigenetic information for further studying bovine embryonic development and cellular reprogramming.http://www.sciencedirect.com/science/article/pii/S209531191661643XbovineDNA methylationoctamer-binding transcription factor 4 (Oct4)exongerm layer |
| spellingShingle | Xin-yu ZHOU Liang-liang LIU Wen-chao JIA Chuan-ying PAN Methylation profile of bovine Oct4 gene coding region in relation to three germ layers Journal of Integrative Agriculture bovine DNA methylation octamer-binding transcription factor 4 (Oct4) exon germ layer |
| title | Methylation profile of bovine Oct4 gene coding region in relation to three germ layers |
| title_full | Methylation profile of bovine Oct4 gene coding region in relation to three germ layers |
| title_fullStr | Methylation profile of bovine Oct4 gene coding region in relation to three germ layers |
| title_full_unstemmed | Methylation profile of bovine Oct4 gene coding region in relation to three germ layers |
| title_short | Methylation profile of bovine Oct4 gene coding region in relation to three germ layers |
| title_sort | methylation profile of bovine oct4 gene coding region in relation to three germ layers |
| topic | bovine DNA methylation octamer-binding transcription factor 4 (Oct4) exon germ layer |
| url | http://www.sciencedirect.com/science/article/pii/S209531191661643X |
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