Photodynamic antimicrobial chemotherapy in mice with Pseudomonas aeruginosa-infected wounds.
This study examined the antibacterial effect of protoporphyrin IX-ethylenediamine derivative (PPIX-ED)-mediated photodynamic antimicrobial chemotherapy (PPIX-ED-PACT) against Pseudomonas aeruginosa in vitro and in vivo. PPIX-ED potently inhibited the growth of Pseudomonas aeruginosa by inducing reac...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2020-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0237851 |
| _version_ | 1819038057709436928 |
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| author | Zhan-Juan Zhao Zeng-Ping Xu Ying-Ying Ma Jin-Duo Ma Ge Hong |
| author_facet | Zhan-Juan Zhao Zeng-Ping Xu Ying-Ying Ma Jin-Duo Ma Ge Hong |
| author_sort | Zhan-Juan Zhao |
| collection | DOAJ |
| description | This study examined the antibacterial effect of protoporphyrin IX-ethylenediamine derivative (PPIX-ED)-mediated photodynamic antimicrobial chemotherapy (PPIX-ED-PACT) against Pseudomonas aeruginosa in vitro and in vivo. PPIX-ED potently inhibited the growth of Pseudomonas aeruginosa by inducing reactive oxygen species production via photoactivation. Atomic force microscopy revealed that PPIX-ED-PACT induced the leakage of bacterial content by degrading the bacterial membrane and wall. As revealed using acridine orange/ethidium bromide staining, PPIX-ED-PACT altered the permeability of the bacterial membrane. In addition, the antibacterial effect of PPIX-ED-PACT was demonstrated in an in vivo model of P. aeruginosa-infected wounds. PPIX-ED (100 μM) decreased the number of P. aeruginosa colony-forming units by 4.2 log10. Moreover, histological analysis illustrated that the wound healing rate was 98% on day 14 after treatment, which was 10% higher than that in the control group. According to the present findings, PPIX-ED-PACT can effectively inhibit the growth of P. aeruginosa in vitro and in vivo. |
| first_indexed | 2024-12-21T08:31:16Z |
| format | Article |
| id | doaj.art-ce1354e190ba4071bdff2714989b0b91 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T08:31:16Z |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-ce1354e190ba4071bdff2714989b0b912022-12-21T19:10:12ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-01159e023785110.1371/journal.pone.0237851Photodynamic antimicrobial chemotherapy in mice with Pseudomonas aeruginosa-infected wounds.Zhan-Juan ZhaoZeng-Ping XuYing-Ying MaJin-Duo MaGe HongThis study examined the antibacterial effect of protoporphyrin IX-ethylenediamine derivative (PPIX-ED)-mediated photodynamic antimicrobial chemotherapy (PPIX-ED-PACT) against Pseudomonas aeruginosa in vitro and in vivo. PPIX-ED potently inhibited the growth of Pseudomonas aeruginosa by inducing reactive oxygen species production via photoactivation. Atomic force microscopy revealed that PPIX-ED-PACT induced the leakage of bacterial content by degrading the bacterial membrane and wall. As revealed using acridine orange/ethidium bromide staining, PPIX-ED-PACT altered the permeability of the bacterial membrane. In addition, the antibacterial effect of PPIX-ED-PACT was demonstrated in an in vivo model of P. aeruginosa-infected wounds. PPIX-ED (100 μM) decreased the number of P. aeruginosa colony-forming units by 4.2 log10. Moreover, histological analysis illustrated that the wound healing rate was 98% on day 14 after treatment, which was 10% higher than that in the control group. According to the present findings, PPIX-ED-PACT can effectively inhibit the growth of P. aeruginosa in vitro and in vivo.https://doi.org/10.1371/journal.pone.0237851 |
| spellingShingle | Zhan-Juan Zhao Zeng-Ping Xu Ying-Ying Ma Jin-Duo Ma Ge Hong Photodynamic antimicrobial chemotherapy in mice with Pseudomonas aeruginosa-infected wounds. PLoS ONE |
| title | Photodynamic antimicrobial chemotherapy in mice with Pseudomonas aeruginosa-infected wounds. |
| title_full | Photodynamic antimicrobial chemotherapy in mice with Pseudomonas aeruginosa-infected wounds. |
| title_fullStr | Photodynamic antimicrobial chemotherapy in mice with Pseudomonas aeruginosa-infected wounds. |
| title_full_unstemmed | Photodynamic antimicrobial chemotherapy in mice with Pseudomonas aeruginosa-infected wounds. |
| title_short | Photodynamic antimicrobial chemotherapy in mice with Pseudomonas aeruginosa-infected wounds. |
| title_sort | photodynamic antimicrobial chemotherapy in mice with pseudomonas aeruginosa infected wounds |
| url | https://doi.org/10.1371/journal.pone.0237851 |
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