Carbohydrate metabolism is a determinant for the host specificity of baculovirus infections
Summary: Baculoviruses Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) and Bombyx mori nucleopolyhedrovirus (BmNPV) have highly similar genome sequences but exhibit no overlap in their host range. After baculovirus infects nonpermissive larvae (e.g., AcMNPV infecting B. mori or BmNP...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-01-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004221016187 |
| _version_ | 1818975840524828672 |
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| author | Chih-Hsuan Tsai Yi-Chi Chuang Yun-Heng Lu Chia-Yang Lin Cheng-Kang Tang Sung-Chan Wei Yueh-Lung Wu |
| author_facet | Chih-Hsuan Tsai Yi-Chi Chuang Yun-Heng Lu Chia-Yang Lin Cheng-Kang Tang Sung-Chan Wei Yueh-Lung Wu |
| author_sort | Chih-Hsuan Tsai |
| collection | DOAJ |
| description | Summary: Baculoviruses Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) and Bombyx mori nucleopolyhedrovirus (BmNPV) have highly similar genome sequences but exhibit no overlap in their host range. After baculovirus infects nonpermissive larvae (e.g., AcMNPV infecting B. mori or BmNPV infecting Spodoptera litura), we found that stored carbohydrates, including hemolymph trehalose and fat body glycogen, are rapidly transformed into glucose; enzymes involved in glycolysis and the TCA cycle are upregulated and produce more ATP; adenosine signaling that regulates glycolytic activity is also increased. Subsequently, phagocytosis in cellular immunity and the expression of genes involved in humoral immunity increase significantly. Moreover, inhibiting glycolysis and the expression of gloverins in nonpermissive hosts increased baculovirus infectivity, indicating that the stimulated energy production is designed to support the immune response against infection. Our study highlights that alteration of the host's carbohydrate metabolism is an important factor determining the host specificity of baculoviruses, in addition to viral factors. |
| first_indexed | 2024-12-20T16:02:21Z |
| format | Article |
| id | doaj.art-ce16735bc5684ab1a160bd814f847fb3 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-12-20T16:02:21Z |
| publishDate | 2022-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-ce16735bc5684ab1a160bd814f847fb32022-12-21T19:34:14ZengElsevieriScience2589-00422022-01-01251103648Carbohydrate metabolism is a determinant for the host specificity of baculovirus infectionsChih-Hsuan Tsai0Yi-Chi Chuang1Yun-Heng Lu2Chia-Yang Lin3Cheng-Kang Tang4Sung-Chan Wei5Yueh-Lung Wu6Department of Entomology, National Taiwan University, 27 Lane 113, Roosevelt Road Sec. 4, Taipei 106, TaiwanDepartment of Entomology, National Taiwan University, 27 Lane 113, Roosevelt Road Sec. 4, Taipei 106, TaiwanDepartment of Entomology, National Taiwan University, 27 Lane 113, Roosevelt Road Sec. 4, Taipei 106, TaiwanDepartment of Entomology, National Taiwan University, 27 Lane 113, Roosevelt Road Sec. 4, Taipei 106, TaiwanDepartment of Entomology, National Taiwan University, 27 Lane 113, Roosevelt Road Sec. 4, Taipei 106, TaiwanDepartment of Entomology, National Taiwan University, 27 Lane 113, Roosevelt Road Sec. 4, Taipei 106, TaiwanDepartment of Entomology, National Taiwan University, 27 Lane 113, Roosevelt Road Sec. 4, Taipei 106, Taiwan; Corresponding authorSummary: Baculoviruses Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) and Bombyx mori nucleopolyhedrovirus (BmNPV) have highly similar genome sequences but exhibit no overlap in their host range. After baculovirus infects nonpermissive larvae (e.g., AcMNPV infecting B. mori or BmNPV infecting Spodoptera litura), we found that stored carbohydrates, including hemolymph trehalose and fat body glycogen, are rapidly transformed into glucose; enzymes involved in glycolysis and the TCA cycle are upregulated and produce more ATP; adenosine signaling that regulates glycolytic activity is also increased. Subsequently, phagocytosis in cellular immunity and the expression of genes involved in humoral immunity increase significantly. Moreover, inhibiting glycolysis and the expression of gloverins in nonpermissive hosts increased baculovirus infectivity, indicating that the stimulated energy production is designed to support the immune response against infection. Our study highlights that alteration of the host's carbohydrate metabolism is an important factor determining the host specificity of baculoviruses, in addition to viral factors.http://www.sciencedirect.com/science/article/pii/S2589004221016187ImmunologyMicrobiologyVirology |
| spellingShingle | Chih-Hsuan Tsai Yi-Chi Chuang Yun-Heng Lu Chia-Yang Lin Cheng-Kang Tang Sung-Chan Wei Yueh-Lung Wu Carbohydrate metabolism is a determinant for the host specificity of baculovirus infections iScience Immunology Microbiology Virology |
| title | Carbohydrate metabolism is a determinant for the host specificity of baculovirus infections |
| title_full | Carbohydrate metabolism is a determinant for the host specificity of baculovirus infections |
| title_fullStr | Carbohydrate metabolism is a determinant for the host specificity of baculovirus infections |
| title_full_unstemmed | Carbohydrate metabolism is a determinant for the host specificity of baculovirus infections |
| title_short | Carbohydrate metabolism is a determinant for the host specificity of baculovirus infections |
| title_sort | carbohydrate metabolism is a determinant for the host specificity of baculovirus infections |
| topic | Immunology Microbiology Virology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004221016187 |
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