Metabolic Characteristics and Discriminative Diagnosis of Growth Hormone Deficiency and Idiopathic Short Stature in Preadolescents and Adolescents
Growth hormone deficiency (GHD) and idiopathic short stature (ISS) are the most common types of short stature (SS), but little is known about their pathogenesis, and even less is known about the study of adolescent SS. In this study, nuclear magnetic resonance (NMR)-based metabolomic analysis combin...
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MDPI AG
2024-04-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/29/7/1661 |
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| author | Yajie Chang Jing Chen Hongwei Zhu Rong Huang Jinxia Wu Yanyan Lin Quanquan Li Guiping Shen Jianghua Feng |
| author_facet | Yajie Chang Jing Chen Hongwei Zhu Rong Huang Jinxia Wu Yanyan Lin Quanquan Li Guiping Shen Jianghua Feng |
| author_sort | Yajie Chang |
| collection | DOAJ |
| description | Growth hormone deficiency (GHD) and idiopathic short stature (ISS) are the most common types of short stature (SS), but little is known about their pathogenesis, and even less is known about the study of adolescent SS. In this study, nuclear magnetic resonance (NMR)-based metabolomic analysis combined with least absolute shrinkage and selection operator (LASSO) were performed to identify the biomarkers of different types of SS (including 94 preadolescent GHD (PAG), 61 preadolescent ISS (PAI), 43 adolescent GHD (ADG), and 19 adolescent ISS (ADI)), and the receiver operating characteristic curve (ROC) was further used to evaluate the predictive power of potential biomarkers. The results showed that fourteen, eleven, nine, and fifteen metabolites were identified as the potential biomarkers of PAG, PAI, ADG, and ADI compared with their corresponding controls, respectively. The disturbed metabolic pathways in preadolescent SS were mainly carbohydrate metabolism and lipid metabolism, while disorders of amino acid metabolism played an important role in adolescent SS. The combination of aspartate, ethanolamine, phosphocholine, and trimethylamine was screened out to identify PAI from PAG, and alanine, histidine, isobutyrate, methanol, and phosphocholine gave a high classification accuracy for ADI and ADC. The differences in metabolic characteristics between GHD and ISS in preadolescents and adolescents will contribute to the development of individualized clinical treatments in short stature. |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-24T10:38:24Z |
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| series | Molecules |
| spelling | doaj.art-ce1692eae7e845eb8cd43717a09d544d2024-04-12T13:23:42ZengMDPI AGMolecules1420-30492024-04-01297166110.3390/molecules29071661Metabolic Characteristics and Discriminative Diagnosis of Growth Hormone Deficiency and Idiopathic Short Stature in Preadolescents and AdolescentsYajie Chang0Jing Chen1Hongwei Zhu2Rong Huang3Jinxia Wu4Yanyan Lin5Quanquan Li6Guiping Shen7Jianghua Feng8Department of Electronic Science, Fujian Provincial Key Laboratory of Plasma and Magnetic Resonance, Xiamen University, Xiamen 361005, ChinaDepartment of Child Health, Women and Children’s Hospital, School of Medicine, Xiamen University, Xiamen 361003, ChinaEducation Section and Department of Pediatrics, The First Affiliated Hospital of Bengbu Medical University, Bengbu 233004, ChinaDepartment of Child Health, Women and Children’s Hospital, School of Medicine, Xiamen University, Xiamen 361003, ChinaDepartment of Electronic Science, Fujian Provincial Key Laboratory of Plasma and Magnetic Resonance, Xiamen University, Xiamen 361005, ChinaDepartment of Child Health, Women and Children’s Hospital, School of Medicine, Xiamen University, Xiamen 361003, ChinaDepartment of Electronic Science, Fujian Provincial Key Laboratory of Plasma and Magnetic Resonance, Xiamen University, Xiamen 361005, ChinaDepartment of Electronic Science, Fujian Provincial Key Laboratory of Plasma and Magnetic Resonance, Xiamen University, Xiamen 361005, ChinaDepartment of Electronic Science, Fujian Provincial Key Laboratory of Plasma and Magnetic Resonance, Xiamen University, Xiamen 361005, ChinaGrowth hormone deficiency (GHD) and idiopathic short stature (ISS) are the most common types of short stature (SS), but little is known about their pathogenesis, and even less is known about the study of adolescent SS. In this study, nuclear magnetic resonance (NMR)-based metabolomic analysis combined with least absolute shrinkage and selection operator (LASSO) were performed to identify the biomarkers of different types of SS (including 94 preadolescent GHD (PAG), 61 preadolescent ISS (PAI), 43 adolescent GHD (ADG), and 19 adolescent ISS (ADI)), and the receiver operating characteristic curve (ROC) was further used to evaluate the predictive power of potential biomarkers. The results showed that fourteen, eleven, nine, and fifteen metabolites were identified as the potential biomarkers of PAG, PAI, ADG, and ADI compared with their corresponding controls, respectively. The disturbed metabolic pathways in preadolescent SS were mainly carbohydrate metabolism and lipid metabolism, while disorders of amino acid metabolism played an important role in adolescent SS. The combination of aspartate, ethanolamine, phosphocholine, and trimethylamine was screened out to identify PAI from PAG, and alanine, histidine, isobutyrate, methanol, and phosphocholine gave a high classification accuracy for ADI and ADC. The differences in metabolic characteristics between GHD and ISS in preadolescents and adolescents will contribute to the development of individualized clinical treatments in short stature.https://www.mdpi.com/1420-3049/29/7/1661short staturegrowth hormone deficiencyidiopathic short staturemetabolomicsadolescent |
| spellingShingle | Yajie Chang Jing Chen Hongwei Zhu Rong Huang Jinxia Wu Yanyan Lin Quanquan Li Guiping Shen Jianghua Feng Metabolic Characteristics and Discriminative Diagnosis of Growth Hormone Deficiency and Idiopathic Short Stature in Preadolescents and Adolescents Molecules short stature growth hormone deficiency idiopathic short stature metabolomics adolescent |
| title | Metabolic Characteristics and Discriminative Diagnosis of Growth Hormone Deficiency and Idiopathic Short Stature in Preadolescents and Adolescents |
| title_full | Metabolic Characteristics and Discriminative Diagnosis of Growth Hormone Deficiency and Idiopathic Short Stature in Preadolescents and Adolescents |
| title_fullStr | Metabolic Characteristics and Discriminative Diagnosis of Growth Hormone Deficiency and Idiopathic Short Stature in Preadolescents and Adolescents |
| title_full_unstemmed | Metabolic Characteristics and Discriminative Diagnosis of Growth Hormone Deficiency and Idiopathic Short Stature in Preadolescents and Adolescents |
| title_short | Metabolic Characteristics and Discriminative Diagnosis of Growth Hormone Deficiency and Idiopathic Short Stature in Preadolescents and Adolescents |
| title_sort | metabolic characteristics and discriminative diagnosis of growth hormone deficiency and idiopathic short stature in preadolescents and adolescents |
| topic | short stature growth hormone deficiency idiopathic short stature metabolomics adolescent |
| url | https://www.mdpi.com/1420-3049/29/7/1661 |
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