Development of the chromatographic fingerprint analysis of dioscorealides and dioscoreanone from Dioscorea membranacea Pierre
High-performance liquid chromatography-photodiode array detection (HPLC-PDA) was developed for chromatographic fingerprint analysis of the ethanolic extract from Dioscorea membranacea Pierre (known as Hua-Khow-Yen in Thai medicinal plant). Chromatographic fingerprint, together with the contents of t...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Prince of Songkla University
2007-03-01
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| Series: | Songklanakarin Journal of Science and Technology (SJST) |
| Subjects: | |
| Online Access: | http://www.sjst.psu.ac.th/journal/29_Suppl_1_Thaiherb_II_pdf/11Anusak_101-107.pdf |
| _version_ | 1828732030968397824 |
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| author | Anusak Sirikatitham Arunporn Itharat Thitima Chuchom |
| author_facet | Anusak Sirikatitham Arunporn Itharat Thitima Chuchom |
| author_sort | Anusak Sirikatitham |
| collection | DOAJ |
| description | High-performance liquid chromatography-photodiode array detection (HPLC-PDA) was developed for chromatographic fingerprint analysis of the ethanolic extract from Dioscorea membranacea Pierre (known as Hua-Khow-Yen in Thai medicinal plant). Chromatographic fingerprint, together with the contents of the dioscorealide A, dioscorealide B and dioscoreanone, which are three markers of the main constituents were first established and applied for quality control. The samples were separated with reversed phase HPLC by linear gradient elution using water and acetonitrile as mobile phase at a flow rate of 1.0 ml/min and detector wavelength at 245 nm for dioscoreanone and at 270 nm for dioscorealide A and dioscorealide B. This HPLC fingerprint analysis has proven its usefulness for identification and assessment for quality evaluation of D. membranacea extract. |
| first_indexed | 2024-04-12T17:55:03Z |
| format | Article |
| id | doaj.art-ce17329ddc81459aa2065507db8cd458 |
| institution | Directory Open Access Journal |
| issn | 0125-3395 |
| language | English |
| last_indexed | 2024-04-12T17:55:03Z |
| publishDate | 2007-03-01 |
| publisher | Prince of Songkla University |
| record_format | Article |
| series | Songklanakarin Journal of Science and Technology (SJST) |
| spelling | doaj.art-ce17329ddc81459aa2065507db8cd4582022-12-22T03:22:23ZengPrince of Songkla UniversitySongklanakarin Journal of Science and Technology (SJST)0125-33952007-03-0129Suppl.1101107Development of the chromatographic fingerprint analysis of dioscorealides and dioscoreanone from Dioscorea membranacea PierreAnusak SirikatithamArunporn ItharatThitima ChuchomHigh-performance liquid chromatography-photodiode array detection (HPLC-PDA) was developed for chromatographic fingerprint analysis of the ethanolic extract from Dioscorea membranacea Pierre (known as Hua-Khow-Yen in Thai medicinal plant). Chromatographic fingerprint, together with the contents of the dioscorealide A, dioscorealide B and dioscoreanone, which are three markers of the main constituents were first established and applied for quality control. The samples were separated with reversed phase HPLC by linear gradient elution using water and acetonitrile as mobile phase at a flow rate of 1.0 ml/min and detector wavelength at 245 nm for dioscoreanone and at 270 nm for dioscorealide A and dioscorealide B. This HPLC fingerprint analysis has proven its usefulness for identification and assessment for quality evaluation of D. membranacea extract.http://www.sjst.psu.ac.th/journal/29_Suppl_1_Thaiherb_II_pdf/11Anusak_101-107.pdfchromatographic fingerprintDioscorea membranaceadioscorealide Adioscorealide Bdioscoreanone |
| spellingShingle | Anusak Sirikatitham Arunporn Itharat Thitima Chuchom Development of the chromatographic fingerprint analysis of dioscorealides and dioscoreanone from Dioscorea membranacea Pierre Songklanakarin Journal of Science and Technology (SJST) chromatographic fingerprint Dioscorea membranacea dioscorealide A dioscorealide B dioscoreanone |
| title | Development of the chromatographic fingerprint analysis of dioscorealides and dioscoreanone from Dioscorea membranacea Pierre |
| title_full | Development of the chromatographic fingerprint analysis of dioscorealides and dioscoreanone from Dioscorea membranacea Pierre |
| title_fullStr | Development of the chromatographic fingerprint analysis of dioscorealides and dioscoreanone from Dioscorea membranacea Pierre |
| title_full_unstemmed | Development of the chromatographic fingerprint analysis of dioscorealides and dioscoreanone from Dioscorea membranacea Pierre |
| title_short | Development of the chromatographic fingerprint analysis of dioscorealides and dioscoreanone from Dioscorea membranacea Pierre |
| title_sort | development of the chromatographic fingerprint analysis of dioscorealides and dioscoreanone from dioscorea membranacea pierre |
| topic | chromatographic fingerprint Dioscorea membranacea dioscorealide A dioscorealide B dioscoreanone |
| url | http://www.sjst.psu.ac.th/journal/29_Suppl_1_Thaiherb_II_pdf/11Anusak_101-107.pdf |
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