Effect of organic tomato ( Lycopersicon esculentum ) extract on the genotoxicity of doxorubicin in the Drosophila wing spot test
The consumption of organic tomatoes (ORTs) reduces the risk of harmful effects to humans and the environment caused by exposure to toxic agrochemicals. In this study, we used the somatic mutation and recombination test (SMART) of wing spots in Drosophila melanogaster to evaluate the genotoxicity of...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2009-01-01
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| Series: | Genetics and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100020 |
| _version_ | 1811306648566759424 |
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| author | Elaine S. Dutra Cristina D. Dias Bethânia C. de Araújo Antônio J. S. Castro Júlio C. Nepomuceno |
| author_facet | Elaine S. Dutra Cristina D. Dias Bethânia C. de Araújo Antônio J. S. Castro Júlio C. Nepomuceno |
| author_sort | Elaine S. Dutra |
| collection | DOAJ |
| description | The consumption of organic tomatoes (ORTs) reduces the risk of harmful effects to humans and the environment caused by exposure to toxic agrochemicals. In this study, we used the somatic mutation and recombination test (SMART) of wing spots in Drosophila melanogaster to evaluate the genotoxicity of ORT and the effect of cotreatment with ORT on the genotoxicity of Doxorubicin ® (DXR, a cancer chemotherapeutic agent) that is mediated by free radical formation. Standard (ST) cross larvae were treated chronically with solutions containing 25%, 50% or 100% of an aqueous extract of ORT, in the absence and presence of DXR (0.125 mg/mL), and the number of mutant spots on the wings of emergent flies was counted. ORT alone was not genotoxic but enhanced the toxicity of DXR when administered concomitantly with DXR. The ORT-enhanced frequency of spots induced by DXR may have resulted from the interaction of ORT with the enzymatic systems that catalyze the metabolic detoxification of this drug. |
| first_indexed | 2024-04-13T08:48:09Z |
| format | Article |
| id | doaj.art-ce1756fd16164f649be935d66ebfa744 |
| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-04-13T08:48:09Z |
| publishDate | 2009-01-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-ce1756fd16164f649be935d66ebfa7442022-12-22T02:53:35ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852009-01-01321133137Effect of organic tomato ( Lycopersicon esculentum ) extract on the genotoxicity of doxorubicin in the Drosophila wing spot testElaine S. DutraCristina D. DiasBethânia C. de AraújoAntônio J. S. CastroJúlio C. NepomucenoThe consumption of organic tomatoes (ORTs) reduces the risk of harmful effects to humans and the environment caused by exposure to toxic agrochemicals. In this study, we used the somatic mutation and recombination test (SMART) of wing spots in Drosophila melanogaster to evaluate the genotoxicity of ORT and the effect of cotreatment with ORT on the genotoxicity of Doxorubicin ® (DXR, a cancer chemotherapeutic agent) that is mediated by free radical formation. Standard (ST) cross larvae were treated chronically with solutions containing 25%, 50% or 100% of an aqueous extract of ORT, in the absence and presence of DXR (0.125 mg/mL), and the number of mutant spots on the wings of emergent flies was counted. ORT alone was not genotoxic but enhanced the toxicity of DXR when administered concomitantly with DXR. The ORT-enhanced frequency of spots induced by DXR may have resulted from the interaction of ORT with the enzymatic systems that catalyze the metabolic detoxification of this drug.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100020antioxidantsco-genotoxicityDrosophila melanogasterorganic tomatoSMART |
| spellingShingle | Elaine S. Dutra Cristina D. Dias Bethânia C. de Araújo Antônio J. S. Castro Júlio C. Nepomuceno Effect of organic tomato ( Lycopersicon esculentum ) extract on the genotoxicity of doxorubicin in the Drosophila wing spot test Genetics and Molecular Biology antioxidants co-genotoxicity Drosophila melanogaster organic tomato SMART |
| title | Effect of organic tomato ( Lycopersicon esculentum ) extract on the genotoxicity of doxorubicin in the Drosophila wing spot test |
| title_full | Effect of organic tomato ( Lycopersicon esculentum ) extract on the genotoxicity of doxorubicin in the Drosophila wing spot test |
| title_fullStr | Effect of organic tomato ( Lycopersicon esculentum ) extract on the genotoxicity of doxorubicin in the Drosophila wing spot test |
| title_full_unstemmed | Effect of organic tomato ( Lycopersicon esculentum ) extract on the genotoxicity of doxorubicin in the Drosophila wing spot test |
| title_short | Effect of organic tomato ( Lycopersicon esculentum ) extract on the genotoxicity of doxorubicin in the Drosophila wing spot test |
| title_sort | effect of organic tomato lycopersicon esculentum extract on the genotoxicity of doxorubicin in the drosophila wing spot test |
| topic | antioxidants co-genotoxicity Drosophila melanogaster organic tomato SMART |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100020 |
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