Identification and Comprehensive Co-Detection of Necrotic and Viable Aneuploid Cancer Cells in Peripheral Blood
Aneuploid circulating tumor cells (CTCs, CD31<sup>−</sup>) and circulating tumor endothelial cells (CTECs, CD31<sup>+</sup>) exhibit an active interplay in peripheral blood, and play an essential role in tumorigenesis, neoangiogenesis, disease progression, therapy-resistant m...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-10-01
|
| Series: | Cancers |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6694/13/20/5108 |
| _version_ | 1797515103194578944 |
|---|---|
| author | Alexander Y. Lin Daisy Dandan Wang Linda Li Peter Ping Lin |
| author_facet | Alexander Y. Lin Daisy Dandan Wang Linda Li Peter Ping Lin |
| author_sort | Alexander Y. Lin |
| collection | DOAJ |
| description | Aneuploid circulating tumor cells (CTCs, CD31<sup>−</sup>) and circulating tumor endothelial cells (CTECs, CD31<sup>+</sup>) exhibit an active interplay in peripheral blood, and play an essential role in tumorigenesis, neoangiogenesis, disease progression, therapy-resistant minimal residual disease (MRD), cancer metastasis and relapse. Currently, most CTC detection techniques are restricted to the indistinguishable quantification of circulating rare cells, including both necrotic and viable cells in cancer patients. Clinically imperative demands to distinguish and detect live and/or dead non-hematological aneuploid cancer cells in peripheral blood, which will assist in the rapid evaluation of therapeutic effects, real-time monitoring of treatment resistance longitudinally developed along with therapy and the effective detection of post-therapeutic MRD, have not yet been achieved. The integrated subtraction enrichment and immunostaining-fluorescence in situ hybridization (SE-iFISH)-derived novel strategy was developed in this study, aiming to precisely identify and detect live and necrotic cancer cells (NC) enriched from carcinoma patients’ biofluids. The innovative SE-iFISH (NC) provides a meaningful and practical approach to co-detect various viable and necrotic aneuploid CTCs and CTECs. The detected circulating rare cells can be characterized and categorized into diverse subtypes based upon cell viability, morphology, multiple tumor markers’ expression, and the degree of aneuploidy relevant to both malignancy and therapeutic resistance. Each subtype of live or necrotic CTCs and CTECs possesses distinct utility in anti-cancer drug development, translational research, and clinical practice. |
| first_indexed | 2024-03-10T06:40:52Z |
| format | Article |
| id | doaj.art-ce183a4186dd41818694e119011cec45 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-10T06:40:52Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-ce183a4186dd41818694e119011cec452023-11-22T17:40:23ZengMDPI AGCancers2072-66942021-10-011320510810.3390/cancers13205108Identification and Comprehensive Co-Detection of Necrotic and Viable Aneuploid Cancer Cells in Peripheral BloodAlexander Y. Lin0Daisy Dandan Wang1Linda Li2Peter Ping Lin3Cytelligen, San Diego, CA 92121, USACytelligen, San Diego, CA 92121, USACytelligen, San Diego, CA 92121, USACytelligen, San Diego, CA 92121, USAAneuploid circulating tumor cells (CTCs, CD31<sup>−</sup>) and circulating tumor endothelial cells (CTECs, CD31<sup>+</sup>) exhibit an active interplay in peripheral blood, and play an essential role in tumorigenesis, neoangiogenesis, disease progression, therapy-resistant minimal residual disease (MRD), cancer metastasis and relapse. Currently, most CTC detection techniques are restricted to the indistinguishable quantification of circulating rare cells, including both necrotic and viable cells in cancer patients. Clinically imperative demands to distinguish and detect live and/or dead non-hematological aneuploid cancer cells in peripheral blood, which will assist in the rapid evaluation of therapeutic effects, real-time monitoring of treatment resistance longitudinally developed along with therapy and the effective detection of post-therapeutic MRD, have not yet been achieved. The integrated subtraction enrichment and immunostaining-fluorescence in situ hybridization (SE-iFISH)-derived novel strategy was developed in this study, aiming to precisely identify and detect live and necrotic cancer cells (NC) enriched from carcinoma patients’ biofluids. The innovative SE-iFISH (NC) provides a meaningful and practical approach to co-detect various viable and necrotic aneuploid CTCs and CTECs. The detected circulating rare cells can be characterized and categorized into diverse subtypes based upon cell viability, morphology, multiple tumor markers’ expression, and the degree of aneuploidy relevant to both malignancy and therapeutic resistance. Each subtype of live or necrotic CTCs and CTECs possesses distinct utility in anti-cancer drug development, translational research, and clinical practice.https://www.mdpi.com/2072-6694/13/20/5108necrotic CTCs and CTECsrapid evaluation of therapeutic effectsMRDliquid biopsySE-iFISH |
| spellingShingle | Alexander Y. Lin Daisy Dandan Wang Linda Li Peter Ping Lin Identification and Comprehensive Co-Detection of Necrotic and Viable Aneuploid Cancer Cells in Peripheral Blood Cancers necrotic CTCs and CTECs rapid evaluation of therapeutic effects MRD liquid biopsy SE-iFISH |
| title | Identification and Comprehensive Co-Detection of Necrotic and Viable Aneuploid Cancer Cells in Peripheral Blood |
| title_full | Identification and Comprehensive Co-Detection of Necrotic and Viable Aneuploid Cancer Cells in Peripheral Blood |
| title_fullStr | Identification and Comprehensive Co-Detection of Necrotic and Viable Aneuploid Cancer Cells in Peripheral Blood |
| title_full_unstemmed | Identification and Comprehensive Co-Detection of Necrotic and Viable Aneuploid Cancer Cells in Peripheral Blood |
| title_short | Identification and Comprehensive Co-Detection of Necrotic and Viable Aneuploid Cancer Cells in Peripheral Blood |
| title_sort | identification and comprehensive co detection of necrotic and viable aneuploid cancer cells in peripheral blood |
| topic | necrotic CTCs and CTECs rapid evaluation of therapeutic effects MRD liquid biopsy SE-iFISH |
| url | https://www.mdpi.com/2072-6694/13/20/5108 |
| work_keys_str_mv | AT alexanderylin identificationandcomprehensivecodetectionofnecroticandviableaneuploidcancercellsinperipheralblood AT daisydandanwang identificationandcomprehensivecodetectionofnecroticandviableaneuploidcancercellsinperipheralblood AT lindali identificationandcomprehensivecodetectionofnecroticandviableaneuploidcancercellsinperipheralblood AT peterpinglin identificationandcomprehensivecodetectionofnecroticandviableaneuploidcancercellsinperipheralblood |