A Comprehensive Analysis of MicroRNAs in Human Osteoporosis
MicroRNAs (miRNAs) are single-stranded RNA molecules that control gene expression in various processes, such as cancers, Alzheimer’s disease, and bone metabolic diseases. However, the regulatory roles of miRNAs in osteoporosis have not been systematically analyzed. Here, we performed a comprehensive...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-10-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2020.516213/full |
| _version_ | 1819228879223521280 |
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| author | Ying Huai Ying Huai Ying Huai Wenjuan Zhang Wenjuan Zhang Wenjuan Zhang Zhihao Chen Zhihao Chen Zhihao Chen Fan Zhao Fan Zhao Fan Zhao Wei Wang Wei Wang Wei Wang Kai Dang Kai Dang Kai Dang Kaiyue Xue Kaiyue Xue Kaiyue Xue Yongguang Gao Yongguang Gao Yongguang Gao Shanfeng Jiang Shanfeng Jiang Shanfeng Jiang Zhiping Miao Zhiping Miao Zhiping Miao Meng Li Qiang Hao Chu Chen Airong Qian Airong Qian Airong Qian |
| author_facet | Ying Huai Ying Huai Ying Huai Wenjuan Zhang Wenjuan Zhang Wenjuan Zhang Zhihao Chen Zhihao Chen Zhihao Chen Fan Zhao Fan Zhao Fan Zhao Wei Wang Wei Wang Wei Wang Kai Dang Kai Dang Kai Dang Kaiyue Xue Kaiyue Xue Kaiyue Xue Yongguang Gao Yongguang Gao Yongguang Gao Shanfeng Jiang Shanfeng Jiang Shanfeng Jiang Zhiping Miao Zhiping Miao Zhiping Miao Meng Li Qiang Hao Chu Chen Airong Qian Airong Qian Airong Qian |
| author_sort | Ying Huai |
| collection | DOAJ |
| description | MicroRNAs (miRNAs) are single-stranded RNA molecules that control gene expression in various processes, such as cancers, Alzheimer’s disease, and bone metabolic diseases. However, the regulatory roles of miRNAs in osteoporosis have not been systematically analyzed. Here, we performed a comprehensive analysis to identify the differentially expressed miRNAs involved in osteoporosis. MiRNAs associated with osteoporosis were collected through literature retrieval and further screened based on specific inclusion and exclusion criteria. The osteoporosis therapeutic targets of miRNAs were obtained by the integration of miRWalk 3.0 database and five human disease therapeutic target databases. Then, the network analysis and functional enrichment analysis of miRNAs and their targets were performed. As a result, 11 eligible miRNAs were identified highly associated with osteoporosis. MiRNA-mRNA network demonstrated there were the complex mutual interactions between miRNAs and their targets. Besides, ADRB2, AR, ESR1, FGFR1, TRAF6, etc., were identified as the top hub genes in protein-protein interaction (PPI) network. Functional enrichment analysis revealed that miRNAs and their targets were mainly mapped on processes associated with bone and immune system, such as bone remolding, bone mineralization, PI3K/AKt, TNF signaling pathways and Th17 cell differentiation. RT-PCR results showed that the expression of miR-335-3p was significantly down-regulated in hind limb unloading (HLU) mice tibia samples compared with controls, the remaining 10 miRNAs were significantly up-regulated after HLU (P < 0.01). In summary, we identified 11 differentially expressed miRNAs and their hub target genes in osteoporosis, which may be novel diagnostic biomarkers for osteoporosis. |
| first_indexed | 2024-12-23T11:04:17Z |
| format | Article |
| id | doaj.art-ce1b3658a899406486676ab3a29f3ac7 |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-12-23T11:04:17Z |
| publishDate | 2020-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-ce1b3658a899406486676ab3a29f3ac72022-12-21T17:49:32ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922020-10-011110.3389/fendo.2020.516213516213A Comprehensive Analysis of MicroRNAs in Human OsteoporosisYing Huai0Ying Huai1Ying Huai2Wenjuan Zhang3Wenjuan Zhang4Wenjuan Zhang5Zhihao Chen6Zhihao Chen7Zhihao Chen8Fan Zhao9Fan Zhao10Fan Zhao11Wei Wang12Wei Wang13Wei Wang14Kai Dang15Kai Dang16Kai Dang17Kaiyue Xue18Kaiyue Xue19Kaiyue Xue20Yongguang Gao21Yongguang Gao22Yongguang Gao23Shanfeng Jiang24Shanfeng Jiang25Shanfeng Jiang26Zhiping Miao27Zhiping Miao28Zhiping Miao29Meng Li30Qiang Hao31Chu Chen32Airong Qian33Airong Qian34Airong Qian35Lab for Bone Metabolism, Key Lab for Space Biosciences and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi’an, ChinaResearch Center for Special Medicine and Health Systems Engineering, School of Life Sciences, Northwestern Polytechnical University, Xi’an, ChinaNPU-UAB Joint Laboratory for Bone Metabolism, School of Life Sciences, Northwestern Polytechnical University, Xi’an, ChinaLab for Bone Metabolism, Key Lab for Space Biosciences and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi’an, ChinaResearch Center for Special Medicine and Health Systems Engineering, School of Life Sciences, Northwestern Polytechnical University, Xi’an, ChinaNPU-UAB Joint Laboratory for Bone Metabolism, School of Life Sciences, Northwestern Polytechnical University, Xi’an, ChinaLab for Bone Metabolism, Key Lab for Space Biosciences and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi’an, ChinaResearch Center for Special Medicine and Health Systems Engineering, School of Life Sciences, Northwestern Polytechnical University, Xi’an, ChinaNPU-UAB Joint Laboratory for Bone Metabolism, School of Life Sciences, Northwestern Polytechnical University, Xi’an, ChinaLab for Bone Metabolism, Key Lab for Space Biosciences and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi’an, ChinaResearch Center for Special Medicine and Health Systems Engineering, School of Life Sciences, Northwestern Polytechnical University, Xi’an, ChinaNPU-UAB Joint Laboratory for Bone Metabolism, School of Life Sciences, Northwestern Polytechnical University, Xi’an, ChinaLab for Bone Metabolism, Key Lab for Space Biosciences and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi’an, ChinaResearch Center for Special Medicine and Health Systems Engineering, School of Life Sciences, Northwestern Polytechnical University, Xi’an, ChinaNPU-UAB Joint Laboratory for Bone Metabolism, School of Life Sciences, Northwestern Polytechnical University, Xi’an, ChinaLab for Bone Metabolism, Key Lab for Space Biosciences and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi’an, ChinaResearch Center for Special Medicine and Health Systems Engineering, School of Life Sciences, Northwestern Polytechnical University, Xi’an, ChinaNPU-UAB Joint Laboratory for Bone Metabolism, School of Life Sciences, Northwestern Polytechnical University, Xi’an, ChinaLab for Bone Metabolism, Key Lab for Space Biosciences and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi’an, ChinaResearch Center for Special Medicine and Health Systems Engineering, School of Life Sciences, Northwestern Polytechnical University, Xi’an, ChinaNPU-UAB Joint Laboratory for Bone Metabolism, School of Life Sciences, Northwestern Polytechnical University, Xi’an, ChinaLab for Bone Metabolism, Key Lab for Space Biosciences and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi’an, ChinaResearch Center for Special Medicine and Health Systems Engineering, School of Life Sciences, Northwestern Polytechnical University, Xi’an, ChinaNPU-UAB Joint Laboratory for Bone Metabolism, School of Life Sciences, Northwestern Polytechnical University, Xi’an, ChinaLab for Bone Metabolism, Key Lab for Space Biosciences and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi’an, ChinaResearch Center for Special Medicine and Health Systems Engineering, School of Life Sciences, Northwestern Polytechnical University, Xi’an, ChinaNPU-UAB Joint Laboratory for Bone Metabolism, School of Life Sciences, Northwestern Polytechnical University, Xi’an, ChinaLab for Bone Metabolism, Key Lab for Space Biosciences and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi’an, ChinaResearch Center for Special Medicine and Health Systems Engineering, School of Life Sciences, Northwestern Polytechnical University, Xi’an, ChinaNPU-UAB Joint Laboratory for Bone Metabolism, School of Life Sciences, Northwestern Polytechnical University, Xi’an, ChinaState Key Laboratory of Cancer Biology, Biotechnology Center, School of Pharmacy, Fourth Military Medical University, Xi’an, ChinaState Key Laboratory of Cancer Biology, Biotechnology Center, School of Pharmacy, Fourth Military Medical University, Xi’an, ChinaClinical Laboratory of Honghui Hospital, Xi’an JiaoTong University College of Medicine, Xi’an, ChinaLab for Bone Metabolism, Key Lab for Space Biosciences and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi’an, ChinaResearch Center for Special Medicine and Health Systems Engineering, School of Life Sciences, Northwestern Polytechnical University, Xi’an, ChinaNPU-UAB Joint Laboratory for Bone Metabolism, School of Life Sciences, Northwestern Polytechnical University, Xi’an, ChinaMicroRNAs (miRNAs) are single-stranded RNA molecules that control gene expression in various processes, such as cancers, Alzheimer’s disease, and bone metabolic diseases. However, the regulatory roles of miRNAs in osteoporosis have not been systematically analyzed. Here, we performed a comprehensive analysis to identify the differentially expressed miRNAs involved in osteoporosis. MiRNAs associated with osteoporosis were collected through literature retrieval and further screened based on specific inclusion and exclusion criteria. The osteoporosis therapeutic targets of miRNAs were obtained by the integration of miRWalk 3.0 database and five human disease therapeutic target databases. Then, the network analysis and functional enrichment analysis of miRNAs and their targets were performed. As a result, 11 eligible miRNAs were identified highly associated with osteoporosis. MiRNA-mRNA network demonstrated there were the complex mutual interactions between miRNAs and their targets. Besides, ADRB2, AR, ESR1, FGFR1, TRAF6, etc., were identified as the top hub genes in protein-protein interaction (PPI) network. Functional enrichment analysis revealed that miRNAs and their targets were mainly mapped on processes associated with bone and immune system, such as bone remolding, bone mineralization, PI3K/AKt, TNF signaling pathways and Th17 cell differentiation. RT-PCR results showed that the expression of miR-335-3p was significantly down-regulated in hind limb unloading (HLU) mice tibia samples compared with controls, the remaining 10 miRNAs were significantly up-regulated after HLU (P < 0.01). In summary, we identified 11 differentially expressed miRNAs and their hub target genes in osteoporosis, which may be novel diagnostic biomarkers for osteoporosis.https://www.frontiersin.org/articles/10.3389/fendo.2020.516213/fullosteoporosismicroRNAsbioinformatics analysisbiomarkertarget genes |
| spellingShingle | Ying Huai Ying Huai Ying Huai Wenjuan Zhang Wenjuan Zhang Wenjuan Zhang Zhihao Chen Zhihao Chen Zhihao Chen Fan Zhao Fan Zhao Fan Zhao Wei Wang Wei Wang Wei Wang Kai Dang Kai Dang Kai Dang Kaiyue Xue Kaiyue Xue Kaiyue Xue Yongguang Gao Yongguang Gao Yongguang Gao Shanfeng Jiang Shanfeng Jiang Shanfeng Jiang Zhiping Miao Zhiping Miao Zhiping Miao Meng Li Qiang Hao Chu Chen Airong Qian Airong Qian Airong Qian A Comprehensive Analysis of MicroRNAs in Human Osteoporosis Frontiers in Endocrinology osteoporosis microRNAs bioinformatics analysis biomarker target genes |
| title | A Comprehensive Analysis of MicroRNAs in Human Osteoporosis |
| title_full | A Comprehensive Analysis of MicroRNAs in Human Osteoporosis |
| title_fullStr | A Comprehensive Analysis of MicroRNAs in Human Osteoporosis |
| title_full_unstemmed | A Comprehensive Analysis of MicroRNAs in Human Osteoporosis |
| title_short | A Comprehensive Analysis of MicroRNAs in Human Osteoporosis |
| title_sort | comprehensive analysis of micrornas in human osteoporosis |
| topic | osteoporosis microRNAs bioinformatics analysis biomarker target genes |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2020.516213/full |
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