Use of sialic acid as a complementary method in the cytological diagnosis: Preliminary study
Introduction: Cellular morphology does not allow, in many cases, to safely establish a diagnosis of malignancy or benignity. Sialic acid is found in the membranes of well-differentiated mature cells, normally located in the alpha-2,3 position. During tumor progression, changes occur in glycosylation...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2018-01-01
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| Series: | Journal of Cytology |
| Subjects: | |
| Online Access: | http://www.jcytol.org/article.asp?issn=0970-9371;year=2018;volume=35;issue=4;spage=242;epage=246;aulast=Rocher |
| _version_ | 1818285570263089152 |
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| author | Adriana E Rocher Anabela A. G. Angeleri Fernando Guerra Luis A Palaoro |
| author_facet | Adriana E Rocher Anabela A. G. Angeleri Fernando Guerra Luis A Palaoro |
| author_sort | Adriana E Rocher |
| collection | DOAJ |
| description | Introduction: Cellular morphology does not allow, in many cases, to safely establish a diagnosis of malignancy or benignity. Sialic acid is found in the membranes of well-differentiated mature cells, normally located in the alpha-2,3 position. During tumor progression, changes occur in glycosylation of proteins and lipids, including alterations in the sialylation patterns of tumor cells. Objective: To confirm the overexpression of alpha-2,6 sialinization in exfoliated cells of body fluids and bronchoalveolar lavage (BAL) as a malignant indicator mechanism, using glycan-binding lectins. Materials and Methods: Thirty samples (20 effusion liquids and 10 BAL) diagnosed by Giemsa and Papanicolaou staining as negative and positive for malignancy, were studied. They were then stained with fluorescein-labeled Sambucus nigra lectin (Sigma Chemicals, USA), which specifically recognizes sialic acid in alpha-2,6 position. The fluorescence obtained at 515 nm evidenced the presence of sialic acid in the 2,6 position. Results: Negative body fluids for malignancy showed a fine and homogeneous fluorescence pattern for reactive mesothelial cells. Neoplastic cells revealed a thick, heterogeneous pattern. In BAL, benign hyperplastic cells showed a homogeneous fine pattern while neoplastic cells showed a thick and heterogeneous fluorescence pattern. The pattern described was observed in all cases in the cell membrane. Conclusion: It was observed that the change in sialic acid conformation detected through Sambucus nigra Lectin could be used as a complementary method for the diagnosis of malignancy in different cytological samples. |
| first_indexed | 2024-12-13T01:10:48Z |
| format | Article |
| id | doaj.art-ce1bb9d4e6624f7190903be10cbf2e58 |
| institution | Directory Open Access Journal |
| issn | 0970-9371 |
| language | English |
| last_indexed | 2024-12-13T01:10:48Z |
| publishDate | 2018-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Cytology |
| spelling | doaj.art-ce1bb9d4e6624f7190903be10cbf2e582022-12-22T00:04:29ZengWolters Kluwer Medknow PublicationsJournal of Cytology0970-93712018-01-0135424224610.4103/JOC.JOC_161_17Use of sialic acid as a complementary method in the cytological diagnosis: Preliminary studyAdriana E RocherAnabela A. G. AngeleriFernando GuerraLuis A PalaoroIntroduction: Cellular morphology does not allow, in many cases, to safely establish a diagnosis of malignancy or benignity. Sialic acid is found in the membranes of well-differentiated mature cells, normally located in the alpha-2,3 position. During tumor progression, changes occur in glycosylation of proteins and lipids, including alterations in the sialylation patterns of tumor cells. Objective: To confirm the overexpression of alpha-2,6 sialinization in exfoliated cells of body fluids and bronchoalveolar lavage (BAL) as a malignant indicator mechanism, using glycan-binding lectins. Materials and Methods: Thirty samples (20 effusion liquids and 10 BAL) diagnosed by Giemsa and Papanicolaou staining as negative and positive for malignancy, were studied. They were then stained with fluorescein-labeled Sambucus nigra lectin (Sigma Chemicals, USA), which specifically recognizes sialic acid in alpha-2,6 position. The fluorescence obtained at 515 nm evidenced the presence of sialic acid in the 2,6 position. Results: Negative body fluids for malignancy showed a fine and homogeneous fluorescence pattern for reactive mesothelial cells. Neoplastic cells revealed a thick, heterogeneous pattern. In BAL, benign hyperplastic cells showed a homogeneous fine pattern while neoplastic cells showed a thick and heterogeneous fluorescence pattern. The pattern described was observed in all cases in the cell membrane. Conclusion: It was observed that the change in sialic acid conformation detected through Sambucus nigra Lectin could be used as a complementary method for the diagnosis of malignancy in different cytological samples.http://www.jcytol.org/article.asp?issn=0970-9371;year=2018;volume=35;issue=4;spage=242;epage=246;aulast=RocherLectinsmesothelial cellsneoplastic cellssialic acid |
| spellingShingle | Adriana E Rocher Anabela A. G. Angeleri Fernando Guerra Luis A Palaoro Use of sialic acid as a complementary method in the cytological diagnosis: Preliminary study Journal of Cytology Lectins mesothelial cells neoplastic cells sialic acid |
| title | Use of sialic acid as a complementary method in the cytological diagnosis: Preliminary study |
| title_full | Use of sialic acid as a complementary method in the cytological diagnosis: Preliminary study |
| title_fullStr | Use of sialic acid as a complementary method in the cytological diagnosis: Preliminary study |
| title_full_unstemmed | Use of sialic acid as a complementary method in the cytological diagnosis: Preliminary study |
| title_short | Use of sialic acid as a complementary method in the cytological diagnosis: Preliminary study |
| title_sort | use of sialic acid as a complementary method in the cytological diagnosis preliminary study |
| topic | Lectins mesothelial cells neoplastic cells sialic acid |
| url | http://www.jcytol.org/article.asp?issn=0970-9371;year=2018;volume=35;issue=4;spage=242;epage=246;aulast=Rocher |
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