An Assessment of the AdenoPlus Point-of-Care Test for Diagnosing Adenoviral Conjunctivitis and Its Effect on Antibiotic Stewardship
Objective: To determine the sensitivity and specificity of the AdenoPlus test compared with real-time polymerase chain reaction (PCR) and to determine whether there was a reduction in antibiotic prescriptions with the use of AdenoPlus compared with the previous year. Patients and Methods: A total of...
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| Format: | Article |
| Language: | English |
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Elsevier
2017-09-01
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| Series: | Mayo Clinic Proceedings: Innovations, Quality & Outcomes |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2542454817300310 |
| _version_ | 1819230436773068800 |
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| author | Kelly K. Holtz, DNP, APRN, CNP Kalie R. Townsend, DNP, APRN, CNP Joseph W. Furst, MD Jane F. Myers, MSN, APRN, CNP Matthew J. Binnicker, PhD, D(ABMM) Stephanie M. Quigg, MS Julie A. Maxson, BA Mark J. Espy, MS |
| author_facet | Kelly K. Holtz, DNP, APRN, CNP Kalie R. Townsend, DNP, APRN, CNP Joseph W. Furst, MD Jane F. Myers, MSN, APRN, CNP Matthew J. Binnicker, PhD, D(ABMM) Stephanie M. Quigg, MS Julie A. Maxson, BA Mark J. Espy, MS |
| author_sort | Kelly K. Holtz, DNP, APRN, CNP |
| collection | DOAJ |
| description | Objective: To determine the sensitivity and specificity of the AdenoPlus test compared with real-time polymerase chain reaction (PCR) and to determine whether there was a reduction in antibiotic prescriptions with the use of AdenoPlus compared with the previous year. Patients and Methods: A total of 125 patients with suspected infectious conjunctivitis were accrued from June 4, 2015, through September 27, 2015. Forty-six participants from the prospective cohort completed both AdenoPlus and PCR testing. Two hundred fifty age-matched individuals were in the retrospective cohort. Results: There was a significant reduction in the percentage of patients who received an antibiotic ophthalmic prescription in the prospective cohort vs the retrospective cohort (32% vs 45%; χ2 P=.01). AdenoPlus test sensitivity was 50% (5 of 10) and specificity was 92% (33 of 36) compared with real-time PCR testing. Conclusion: The AdenoPlus test has high specificity for diagnosing adenoviral conjunctivitis but lower sensitivity than has been previously published. These data suggest that negative AdenoPlus results should be confirmed by real-time PCR owing to the low overall sensitivity of AdenoPlus observed. |
| first_indexed | 2024-12-23T11:29:03Z |
| format | Article |
| id | doaj.art-ce1ea838e9d441c6b26437eb78aafaaf |
| institution | Directory Open Access Journal |
| issn | 2542-4548 |
| language | English |
| last_indexed | 2024-12-23T11:29:03Z |
| publishDate | 2017-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Mayo Clinic Proceedings: Innovations, Quality & Outcomes |
| spelling | doaj.art-ce1ea838e9d441c6b26437eb78aafaaf2022-12-21T17:48:52ZengElsevierMayo Clinic Proceedings: Innovations, Quality & Outcomes2542-45482017-09-0112170175An Assessment of the AdenoPlus Point-of-Care Test for Diagnosing Adenoviral Conjunctivitis and Its Effect on Antibiotic StewardshipKelly K. Holtz, DNP, APRN, CNP0Kalie R. Townsend, DNP, APRN, CNP1Joseph W. Furst, MD2Jane F. Myers, MSN, APRN, CNP3Matthew J. Binnicker, PhD, D(ABMM)4Stephanie M. Quigg, MS5Julie A. Maxson, BA6Mark J. Espy, MS7Department of Family Medicine, Mayo Clinic, Rochester, MN; Correspondence: Address to Kelly K. Holtz, DNP, APRN, CNP, Department of Family Medicine, Mayo Clinic, 200 First St SW, Rochester, MN 55905.Department of Family Medicine, Mayo Clinic, Rochester, MN; Urgent Care and Emergency Medicine Department, Mayo Clinic Health System Red Cedar, Menomonie, WIDepartment of Family Medicine, Mayo Clinic, Rochester, MNDepartment of Family Medicine, Mayo Clinic, Rochester, MNDivision of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MNDepartment of Family Medicine, Mayo Clinic, Rochester, MNDepartment of Family Medicine, Mayo Clinic, Rochester, MNDivision of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MNObjective: To determine the sensitivity and specificity of the AdenoPlus test compared with real-time polymerase chain reaction (PCR) and to determine whether there was a reduction in antibiotic prescriptions with the use of AdenoPlus compared with the previous year. Patients and Methods: A total of 125 patients with suspected infectious conjunctivitis were accrued from June 4, 2015, through September 27, 2015. Forty-six participants from the prospective cohort completed both AdenoPlus and PCR testing. Two hundred fifty age-matched individuals were in the retrospective cohort. Results: There was a significant reduction in the percentage of patients who received an antibiotic ophthalmic prescription in the prospective cohort vs the retrospective cohort (32% vs 45%; χ2 P=.01). AdenoPlus test sensitivity was 50% (5 of 10) and specificity was 92% (33 of 36) compared with real-time PCR testing. Conclusion: The AdenoPlus test has high specificity for diagnosing adenoviral conjunctivitis but lower sensitivity than has been previously published. These data suggest that negative AdenoPlus results should be confirmed by real-time PCR owing to the low overall sensitivity of AdenoPlus observed.http://www.sciencedirect.com/science/article/pii/S2542454817300310 |
| spellingShingle | Kelly K. Holtz, DNP, APRN, CNP Kalie R. Townsend, DNP, APRN, CNP Joseph W. Furst, MD Jane F. Myers, MSN, APRN, CNP Matthew J. Binnicker, PhD, D(ABMM) Stephanie M. Quigg, MS Julie A. Maxson, BA Mark J. Espy, MS An Assessment of the AdenoPlus Point-of-Care Test for Diagnosing Adenoviral Conjunctivitis and Its Effect on Antibiotic Stewardship Mayo Clinic Proceedings: Innovations, Quality & Outcomes |
| title | An Assessment of the AdenoPlus Point-of-Care Test for Diagnosing Adenoviral Conjunctivitis and Its Effect on Antibiotic Stewardship |
| title_full | An Assessment of the AdenoPlus Point-of-Care Test for Diagnosing Adenoviral Conjunctivitis and Its Effect on Antibiotic Stewardship |
| title_fullStr | An Assessment of the AdenoPlus Point-of-Care Test for Diagnosing Adenoviral Conjunctivitis and Its Effect on Antibiotic Stewardship |
| title_full_unstemmed | An Assessment of the AdenoPlus Point-of-Care Test for Diagnosing Adenoviral Conjunctivitis and Its Effect on Antibiotic Stewardship |
| title_short | An Assessment of the AdenoPlus Point-of-Care Test for Diagnosing Adenoviral Conjunctivitis and Its Effect on Antibiotic Stewardship |
| title_sort | assessment of the adenoplus point of care test for diagnosing adenoviral conjunctivitis and its effect on antibiotic stewardship |
| url | http://www.sciencedirect.com/science/article/pii/S2542454817300310 |
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