Expression and Association of the Yersinia pestis Translocon Proteins, YopB and YopD, Are Facilitated by Nanolipoprotein Particles.
Yersinia pestis enters host cells and evades host defenses, in part, through interactions between Yersinia pestis proteins and host membranes. One such interaction is through the type III secretion system, which uses a highly conserved and ordered complex for Yersinia pestis outer membrane effector...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4807764?pdf=render |
| _version_ | 1818909198135590912 |
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| author | Matthew A Coleman Jenny A Cappuccio Craig D Blanchette Tingjuan Gao Erin S Arroyo Angela K Hinz Feliza A Bourguet Brent Segelke Paul D Hoeprich Thomas Huser Ted A Laurence Vladimir L Motin Brett A Chromy |
| author_facet | Matthew A Coleman Jenny A Cappuccio Craig D Blanchette Tingjuan Gao Erin S Arroyo Angela K Hinz Feliza A Bourguet Brent Segelke Paul D Hoeprich Thomas Huser Ted A Laurence Vladimir L Motin Brett A Chromy |
| author_sort | Matthew A Coleman |
| collection | DOAJ |
| description | Yersinia pestis enters host cells and evades host defenses, in part, through interactions between Yersinia pestis proteins and host membranes. One such interaction is through the type III secretion system, which uses a highly conserved and ordered complex for Yersinia pestis outer membrane effector protein translocation called the injectisome. The portion of the injectisome that interacts directly with host cell membranes is referred to as the translocon. The translocon is believed to form a pore allowing effector molecules to enter host cells. To facilitate mechanistic studies of the translocon, we have developed a cell-free approach for expressing translocon pore proteins as a complex supported in a bilayer membrane mimetic nano-scaffold known as a nanolipoprotein particle (NLP) Initial results show cell-free expression of Yersinia pestis outer membrane proteins YopB and YopD was enhanced in the presence of liposomes. However, these complexes tended to aggregate and precipitate. With the addition of co-expressed (NLP) forming components, the YopB and/or YopD complex was rendered soluble, increasing the yield of protein for biophysical studies. Biophysical methods such as Atomic Force Microscopy and Fluorescence Correlation Spectroscopy were used to confirm that the soluble YopB/D complex was associated with NLPs. An interaction between the YopB/D complex and NLP was validated by immunoprecipitation. The YopB/D translocon complex embedded in a NLP provides a platform for protein interaction studies between pathogen and host proteins. These studies will help elucidate the poorly understood mechanism which enables this pathogen to inject effector proteins into host cells, thus evading host defenses. |
| first_indexed | 2024-12-19T22:23:06Z |
| format | Article |
| id | doaj.art-ce20e916630041188cd3ee34a7e0cc71 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-19T22:23:06Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-ce20e916630041188cd3ee34a7e0cc712022-12-21T20:03:34ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01113e015016610.1371/journal.pone.0150166Expression and Association of the Yersinia pestis Translocon Proteins, YopB and YopD, Are Facilitated by Nanolipoprotein Particles.Matthew A ColemanJenny A CappuccioCraig D BlanchetteTingjuan GaoErin S ArroyoAngela K HinzFeliza A BourguetBrent SegelkePaul D HoeprichThomas HuserTed A LaurenceVladimir L MotinBrett A ChromyYersinia pestis enters host cells and evades host defenses, in part, through interactions between Yersinia pestis proteins and host membranes. One such interaction is through the type III secretion system, which uses a highly conserved and ordered complex for Yersinia pestis outer membrane effector protein translocation called the injectisome. The portion of the injectisome that interacts directly with host cell membranes is referred to as the translocon. The translocon is believed to form a pore allowing effector molecules to enter host cells. To facilitate mechanistic studies of the translocon, we have developed a cell-free approach for expressing translocon pore proteins as a complex supported in a bilayer membrane mimetic nano-scaffold known as a nanolipoprotein particle (NLP) Initial results show cell-free expression of Yersinia pestis outer membrane proteins YopB and YopD was enhanced in the presence of liposomes. However, these complexes tended to aggregate and precipitate. With the addition of co-expressed (NLP) forming components, the YopB and/or YopD complex was rendered soluble, increasing the yield of protein for biophysical studies. Biophysical methods such as Atomic Force Microscopy and Fluorescence Correlation Spectroscopy were used to confirm that the soluble YopB/D complex was associated with NLPs. An interaction between the YopB/D complex and NLP was validated by immunoprecipitation. The YopB/D translocon complex embedded in a NLP provides a platform for protein interaction studies between pathogen and host proteins. These studies will help elucidate the poorly understood mechanism which enables this pathogen to inject effector proteins into host cells, thus evading host defenses.http://europepmc.org/articles/PMC4807764?pdf=render |
| spellingShingle | Matthew A Coleman Jenny A Cappuccio Craig D Blanchette Tingjuan Gao Erin S Arroyo Angela K Hinz Feliza A Bourguet Brent Segelke Paul D Hoeprich Thomas Huser Ted A Laurence Vladimir L Motin Brett A Chromy Expression and Association of the Yersinia pestis Translocon Proteins, YopB and YopD, Are Facilitated by Nanolipoprotein Particles. PLoS ONE |
| title | Expression and Association of the Yersinia pestis Translocon Proteins, YopB and YopD, Are Facilitated by Nanolipoprotein Particles. |
| title_full | Expression and Association of the Yersinia pestis Translocon Proteins, YopB and YopD, Are Facilitated by Nanolipoprotein Particles. |
| title_fullStr | Expression and Association of the Yersinia pestis Translocon Proteins, YopB and YopD, Are Facilitated by Nanolipoprotein Particles. |
| title_full_unstemmed | Expression and Association of the Yersinia pestis Translocon Proteins, YopB and YopD, Are Facilitated by Nanolipoprotein Particles. |
| title_short | Expression and Association of the Yersinia pestis Translocon Proteins, YopB and YopD, Are Facilitated by Nanolipoprotein Particles. |
| title_sort | expression and association of the yersinia pestis translocon proteins yopb and yopd are facilitated by nanolipoprotein particles |
| url | http://europepmc.org/articles/PMC4807764?pdf=render |
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