Correlation between clinical signs of feline conjunctivitis and molecular detection of felid herpesvirus-1, feline calicivirus, chlamydophila felis and mycoplasma felis in cats from shelters in Rio de Janeiro
Objectives: To perform molecular diagnosis of microbial agents (FHV-1, FCV, Mycoplasma felis, and Chlamydophila felis) in kittens with conjunctivitis and correlate the clinical signs with clinical severity. Material and Methods: A total of 108 conjunctival swab were collected from kittens without (G...
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| Format: | Article |
| Language: | English |
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Universidade de São Paulo
2017-05-01
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| Series: | Brazilian Journal of Veterinary Research and Animal Science |
| Subjects: | |
| Online Access: | http://www.revistas.usp.br/bjvras/article/view/104588 |
| _version_ | 1828846695346077696 |
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| author | Natasha Baumworcel Ana Maria Barros Soares Sheila Bruna Silva Núbia Karla Oliveira Almeida Tatiana Xavier de Castro |
| author_facet | Natasha Baumworcel Ana Maria Barros Soares Sheila Bruna Silva Núbia Karla Oliveira Almeida Tatiana Xavier de Castro |
| author_sort | Natasha Baumworcel |
| collection | DOAJ |
| description | Objectives: To perform molecular diagnosis of microbial agents (FHV-1, FCV, Mycoplasma felis, and Chlamydophila felis) in kittens with conjunctivitis and correlate the clinical signs with clinical severity. Material and Methods: A total of 108 conjunctival swab were collected from kittens without (G1; n = 40) and with (G2; n = 68) clinical signs of conjunctivitis. Animals from G2 group were scored from 1 (mild) to 4 (severe) according to the severity of conjunctivitis. All samples were submitted to PCR and RT-PCR. Results: FHV-1 was detected in 62/108 (57.4%) of samples, FCV in 40/108 (37.0%), M. felis in 11/108 (10.2%) and C. felis in 26/108 (24.1%). Mixed infections were detected in 39/108 (36.1%). In G1, 28/40 (70.0%) were positive for one or more agents, in G2, 58/68 (85.3%) were positive (P = 0.03). In 1, single infections by FHV-1were found in 21/40 (52.5%) samples, FCV in 2/40 (5.0%), C. felis in 1/40 (2.5%), and no pathogens were detected in 12/40 (30%) of samples, while mixed infections accounted for 29/40 (72.5%) of the cases. In G2, single FHV-1 infections were found in 31/68 (45.6%) samples, FCV in 10/68 (14.7 %), M. felis in 2/68 (3.0%) and C. felis also in 2/68 (3.0%), and no pathogens were detected in 10/68 (14.7%) samples, while mixed infections accounted for 36/68 (52.0%) of the cases. They were categorized as grade 1, 20/68 (29.4%), grade 2, 14/68 (20.6%), grade 3, 21/68 (30.9%) and grade 4, 13/68 (19.1%). The presence of FHV-1 and FCV is equally distributed among the four categories. More severe clinical signs, scores 3 and 4, are related to coinfections by C. felis and M. felis. Conclusions: FHV-1, FCV, C. felis and M. felis were identified in feline conjunctivitis. Co-infections are related to more severe cases of conjunctivitis. Molecular diagnosis is helpful to detect asymptomatic carriers and is a rapid and accurate method to determine the pathogen of feline conjunctivitis. |
| first_indexed | 2024-12-12T21:53:57Z |
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| id | doaj.art-ce231c350eeb4912a39fb54f63f41d4f |
| institution | Directory Open Access Journal |
| issn | 1413-9596 1678-4456 |
| language | English |
| last_indexed | 2024-12-12T21:53:57Z |
| publishDate | 2017-05-01 |
| publisher | Universidade de São Paulo |
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| series | Brazilian Journal of Veterinary Research and Animal Science |
| spelling | doaj.art-ce231c350eeb4912a39fb54f63f41d4f2022-12-22T00:10:43ZengUniversidade de São PauloBrazilian Journal of Veterinary Research and Animal Science1413-95961678-44562017-05-0154110.11606/issn.1678-4456.bjvras.2017.104588113209Correlation between clinical signs of feline conjunctivitis and molecular detection of felid herpesvirus-1, feline calicivirus, chlamydophila felis and mycoplasma felis in cats from shelters in Rio de JaneiroNatasha Baumworcel0Ana Maria Barros Soares1Sheila Bruna Silva2Núbia Karla Oliveira Almeida3Tatiana Xavier de Castro4Universidade Federal Fluminense, Instituto Biomédico, Departamento de Microbiologia e ParasitologiaUniversidade Federal Fluminense, Faculdade de VeterináriaInstituto Biomédico Universidade Federal Fluminense, Departamento de Microbiologia e ParasitologiaUniversidade Federal Fluminense, Instituto de Matemática e Estatística, Departamento de EstatísticaUniversidade Federal Fluminense, Instituto Biomédico, Departamento de Microbiologia e ParasitologiaObjectives: To perform molecular diagnosis of microbial agents (FHV-1, FCV, Mycoplasma felis, and Chlamydophila felis) in kittens with conjunctivitis and correlate the clinical signs with clinical severity. Material and Methods: A total of 108 conjunctival swab were collected from kittens without (G1; n = 40) and with (G2; n = 68) clinical signs of conjunctivitis. Animals from G2 group were scored from 1 (mild) to 4 (severe) according to the severity of conjunctivitis. All samples were submitted to PCR and RT-PCR. Results: FHV-1 was detected in 62/108 (57.4%) of samples, FCV in 40/108 (37.0%), M. felis in 11/108 (10.2%) and C. felis in 26/108 (24.1%). Mixed infections were detected in 39/108 (36.1%). In G1, 28/40 (70.0%) were positive for one or more agents, in G2, 58/68 (85.3%) were positive (P = 0.03). In 1, single infections by FHV-1were found in 21/40 (52.5%) samples, FCV in 2/40 (5.0%), C. felis in 1/40 (2.5%), and no pathogens were detected in 12/40 (30%) of samples, while mixed infections accounted for 29/40 (72.5%) of the cases. In G2, single FHV-1 infections were found in 31/68 (45.6%) samples, FCV in 10/68 (14.7 %), M. felis in 2/68 (3.0%) and C. felis also in 2/68 (3.0%), and no pathogens were detected in 10/68 (14.7%) samples, while mixed infections accounted for 36/68 (52.0%) of the cases. They were categorized as grade 1, 20/68 (29.4%), grade 2, 14/68 (20.6%), grade 3, 21/68 (30.9%) and grade 4, 13/68 (19.1%). The presence of FHV-1 and FCV is equally distributed among the four categories. More severe clinical signs, scores 3 and 4, are related to coinfections by C. felis and M. felis. Conclusions: FHV-1, FCV, C. felis and M. felis were identified in feline conjunctivitis. Co-infections are related to more severe cases of conjunctivitis. Molecular diagnosis is helpful to detect asymptomatic carriers and is a rapid and accurate method to determine the pathogen of feline conjunctivitis.http://www.revistas.usp.br/bjvras/article/view/104588Ocular infectionFelis catusPolymerase chain reactionUpper respiratory tract diseaseEye |
| spellingShingle | Natasha Baumworcel Ana Maria Barros Soares Sheila Bruna Silva Núbia Karla Oliveira Almeida Tatiana Xavier de Castro Correlation between clinical signs of feline conjunctivitis and molecular detection of felid herpesvirus-1, feline calicivirus, chlamydophila felis and mycoplasma felis in cats from shelters in Rio de Janeiro Brazilian Journal of Veterinary Research and Animal Science Ocular infection Felis catus Polymerase chain reaction Upper respiratory tract disease Eye |
| title | Correlation between clinical signs of feline conjunctivitis and molecular detection of felid herpesvirus-1, feline calicivirus, chlamydophila felis and mycoplasma felis in cats from shelters in Rio de Janeiro |
| title_full | Correlation between clinical signs of feline conjunctivitis and molecular detection of felid herpesvirus-1, feline calicivirus, chlamydophila felis and mycoplasma felis in cats from shelters in Rio de Janeiro |
| title_fullStr | Correlation between clinical signs of feline conjunctivitis and molecular detection of felid herpesvirus-1, feline calicivirus, chlamydophila felis and mycoplasma felis in cats from shelters in Rio de Janeiro |
| title_full_unstemmed | Correlation between clinical signs of feline conjunctivitis and molecular detection of felid herpesvirus-1, feline calicivirus, chlamydophila felis and mycoplasma felis in cats from shelters in Rio de Janeiro |
| title_short | Correlation between clinical signs of feline conjunctivitis and molecular detection of felid herpesvirus-1, feline calicivirus, chlamydophila felis and mycoplasma felis in cats from shelters in Rio de Janeiro |
| title_sort | correlation between clinical signs of feline conjunctivitis and molecular detection of felid herpesvirus 1 feline calicivirus chlamydophila felis and mycoplasma felis in cats from shelters in rio de janeiro |
| topic | Ocular infection Felis catus Polymerase chain reaction Upper respiratory tract disease Eye |
| url | http://www.revistas.usp.br/bjvras/article/view/104588 |
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