Evidence of eosinophil extracellular trap cell death in COPD: does it represent the trigger that switches on the disease?
Loli Uribe Echevarría,1 Carolina Leimgruber,2 Jorge García González,1 Alberto Nevado,1 Ruth Álvarez,3 Luciana N García,2 Amado A Quintar,2 Cristina A Maldonado2 1Pneumonology Service, Sanatorio Allende (Nueva Córdoba), Cór...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2017-03-01
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| Series: | International Journal of COPD |
| Subjects: | |
| Online Access: | https://www.dovepress.com/evidence-of-eosinophil-extracellular-trap-cell-death-in-copd-does-it-r-peer-reviewed-article-COPD |
| _version_ | 1818518643765411840 |
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| author | Uribe Echevarría L Leimgruber C García González J Nevado A Álvarez R García LN Quintar AA Maldonado CA |
| author_facet | Uribe Echevarría L Leimgruber C García González J Nevado A Álvarez R García LN Quintar AA Maldonado CA |
| author_sort | Uribe Echevarría L |
| collection | DOAJ |
| description | Loli Uribe Echevarría,1 Carolina Leimgruber,2 Jorge García González,1 Alberto Nevado,1 Ruth Álvarez,3 Luciana N García,2 Amado A Quintar,2 Cristina A Maldonado2 1Pneumonology Service, Sanatorio Allende (Nueva Córdoba), Córdoba, Argentina; 2Electronic Microscopy Center, Instituto de Investigaciones en Ciencias de la Salud (INICSA-CONICET), Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Córdoba, Argentina; 3Smoking Cessation Cente, Nuevo Hospital San Roque of Córdoba, Córdoba, Argentina Abstract: In spite of the numerous studies on chronic obstructive pulmonary disease (COPD), the cellular and molecular basis of the disease’s development remain unclear. Neutrophils and eosinophils are known to be key players in COPD. Recently, neutrophil extracellular trap cell death (NETosis), a mechanism due to decondensation and extrusion of chromatin to form extracellular traps, has been demonstrated in COPD. However, there is limited knowledge about eosinophil extracellular trap cell death (EETosis) and its role in the pathogenesis of COPD. The aim of this study was to evaluate EETosis in stable COPD. Induced sputum obtained from healthy smokers and low exacerbation risk COPD A or B group patients or high exacerbation risk COPD C or D group patients were included. Samples were examined using electron microscopy and immunofluorescence. Healthy smokers (n=10) and COPD A (n=19) group exhibited neutrophilic or paucigranulocytic phenotypes, with NETosis being absent in these patients. In contrast, COPD B (n=29), with eosinophilic or mixed phenotypes, showed EETosis and incipient NETosis. COPD C (n=18) and COPD D groups (n=13) were differentiated from low exacerbation rate-COPD group by the abundant cellular debris, with COPD C group having an eosinophilic pattern and numerous cells undergoing EETosis. A hallmark of this group was the abundant released membranes that often appeared phagocytosed by neutrophils, which coincidentally exhibited early NETosis changes. The COPD D group included patients with a neutrophilic or mixed pattern, with abundant neutrophil extracellular trap-derived material. This study is the first to demonstrate EETosis at different stages of stable COPD. The results suggest a role for eosinophils in COPD pathophysiology, especially at the beginning and during the persistence of the disease, regardless of whether the patient quit smoking, with EETosis debris probably triggering uncontrolled NETosis. The main target of these findings should be young smokers with the potential to develop COPD. Keywords: COPD, eosinophils, neutrophils, NETosis, EETosis, induced sputum |
| first_indexed | 2024-12-11T01:13:02Z |
| format | Article |
| id | doaj.art-ce2330ae69e64416af2df189a8807a74 |
| institution | Directory Open Access Journal |
| issn | 1178-2005 |
| language | English |
| last_indexed | 2024-12-11T01:13:02Z |
| publishDate | 2017-03-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of COPD |
| spelling | doaj.art-ce2330ae69e64416af2df189a8807a742022-12-22T01:25:59ZengDove Medical PressInternational Journal of COPD1178-20052017-03-01Volume 1288589631864Evidence of eosinophil extracellular trap cell death in COPD: does it represent the trigger that switches on the disease?Uribe Echevarría LLeimgruber CGarcía González JNevado AÁlvarez RGarcía LNQuintar AAMaldonado CALoli Uribe Echevarría,1 Carolina Leimgruber,2 Jorge García González,1 Alberto Nevado,1 Ruth Álvarez,3 Luciana N García,2 Amado A Quintar,2 Cristina A Maldonado2 1Pneumonology Service, Sanatorio Allende (Nueva Córdoba), Córdoba, Argentina; 2Electronic Microscopy Center, Instituto de Investigaciones en Ciencias de la Salud (INICSA-CONICET), Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Córdoba, Argentina; 3Smoking Cessation Cente, Nuevo Hospital San Roque of Córdoba, Córdoba, Argentina Abstract: In spite of the numerous studies on chronic obstructive pulmonary disease (COPD), the cellular and molecular basis of the disease’s development remain unclear. Neutrophils and eosinophils are known to be key players in COPD. Recently, neutrophil extracellular trap cell death (NETosis), a mechanism due to decondensation and extrusion of chromatin to form extracellular traps, has been demonstrated in COPD. However, there is limited knowledge about eosinophil extracellular trap cell death (EETosis) and its role in the pathogenesis of COPD. The aim of this study was to evaluate EETosis in stable COPD. Induced sputum obtained from healthy smokers and low exacerbation risk COPD A or B group patients or high exacerbation risk COPD C or D group patients were included. Samples were examined using electron microscopy and immunofluorescence. Healthy smokers (n=10) and COPD A (n=19) group exhibited neutrophilic or paucigranulocytic phenotypes, with NETosis being absent in these patients. In contrast, COPD B (n=29), with eosinophilic or mixed phenotypes, showed EETosis and incipient NETosis. COPD C (n=18) and COPD D groups (n=13) were differentiated from low exacerbation rate-COPD group by the abundant cellular debris, with COPD C group having an eosinophilic pattern and numerous cells undergoing EETosis. A hallmark of this group was the abundant released membranes that often appeared phagocytosed by neutrophils, which coincidentally exhibited early NETosis changes. The COPD D group included patients with a neutrophilic or mixed pattern, with abundant neutrophil extracellular trap-derived material. This study is the first to demonstrate EETosis at different stages of stable COPD. The results suggest a role for eosinophils in COPD pathophysiology, especially at the beginning and during the persistence of the disease, regardless of whether the patient quit smoking, with EETosis debris probably triggering uncontrolled NETosis. The main target of these findings should be young smokers with the potential to develop COPD. Keywords: COPD, eosinophils, neutrophils, NETosis, EETosis, induced sputumhttps://www.dovepress.com/evidence-of-eosinophil-extracellular-trap-cell-death-in-copd-does-it-r-peer-reviewed-article-COPDCOPDeosinophilsneutrophilsNETosisEETosisinduced sputum |
| spellingShingle | Uribe Echevarría L Leimgruber C García González J Nevado A Álvarez R García LN Quintar AA Maldonado CA Evidence of eosinophil extracellular trap cell death in COPD: does it represent the trigger that switches on the disease? International Journal of COPD COPD eosinophils neutrophils NETosis EETosis induced sputum |
| title | Evidence of eosinophil extracellular trap cell death in COPD: does it represent the trigger that switches on the disease? |
| title_full | Evidence of eosinophil extracellular trap cell death in COPD: does it represent the trigger that switches on the disease? |
| title_fullStr | Evidence of eosinophil extracellular trap cell death in COPD: does it represent the trigger that switches on the disease? |
| title_full_unstemmed | Evidence of eosinophil extracellular trap cell death in COPD: does it represent the trigger that switches on the disease? |
| title_short | Evidence of eosinophil extracellular trap cell death in COPD: does it represent the trigger that switches on the disease? |
| title_sort | evidence of eosinophil extracellular trap cell death in copd does it represent the trigger that switches on the disease |
| topic | COPD eosinophils neutrophils NETosis EETosis induced sputum |
| url | https://www.dovepress.com/evidence-of-eosinophil-extracellular-trap-cell-death-in-copd-does-it-r-peer-reviewed-article-COPD |
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