Determination of the dietary lysine requirement by measuring plasma free lysine concentrations in rainbow trout Oncorhynchus mykiss after dorsal aorta cannulation
Abstract This study evaluated the dietary lysine requirement by measuring the plasma free lysine concentrations in rainbow trout, Oncorhynchus mykiss after dorsal aorta cannulation. A basal diet containing 36.6 % crude protein (29.6 % crystalline amino acids mixture, 5 % casein and 2 % gelatin) was...
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| Format: | Article |
| Language: | English |
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The Korean Society of Fisheries and Aquatic Science
2016-03-01
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| Series: | Fisheries and Aquatic Sciences |
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| Online Access: | http://link.springer.com/article/10.1186/s41240-016-0004-1 |
| _version_ | 1818578117561679872 |
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| author | Hyeonho Yun Gunjun Park Imho Ok Kumar Katya Silas Hung Sungchul C. Bai |
| author_facet | Hyeonho Yun Gunjun Park Imho Ok Kumar Katya Silas Hung Sungchul C. Bai |
| author_sort | Hyeonho Yun |
| collection | DOAJ |
| description | Abstract This study evaluated the dietary lysine requirement by measuring the plasma free lysine concentrations in rainbow trout, Oncorhynchus mykiss after dorsal aorta cannulation. A basal diet containing 36.6 % crude protein (29.6 % crystalline amino acids mixture, 5 % casein and 2 % gelatin) was formulated to one of the seven L-amino acid based diets containing graded levels of lysine (0.72, 1.12, 1.52, 1.92, 2.32, 2.72 or 3.52 % dry diet). A total of 35 fish averaging 512 ± 6.8 g (mean ± SD) were randomly distributed into seven groups with five fish in each group. After 48 h of feed deprivation, each group of fish was fed one of the experimental diets by intubation at 1 % body weight. Blood samples were taken at 0, 5 and 24 h after intubation. Post-prandial plasma free lysine concentrations (PPlys, 5 h after intubation) of fish fed diets containing ≥ 2.32 % lysine were higher than those of fish fed diets containing ≤ 1.92 % lysine. Post-absorptive free lysine concentrations (PAlys, 24 h after intubation) of fish fed diets containing 2.32 and 3.52 % lysine were higher than those of fish fed diets containing ≤ 1.52 % lysine. The broken-line regression analysis on the basis of PPlys and PAlys indicated that the lysine requirement of rainbow trout could be 2.34 and 2.20 % in diet. Therefore, these results strongly suggested that the dietary lysine requirement based on the broken-line model analyses of PPlys and PAlys could be greater than 2.2 but less than 2.34 % (corresponding to be 6.01 % ≤, but ≤ 6.39 % in dietary protein basis, respectively) in rainbow trout. Also, these results shown that the quantitative estimation of lysine requirement by using PPlys and PAlys could be an acceptable method in fish. |
| first_indexed | 2024-12-16T06:40:43Z |
| format | Article |
| id | doaj.art-ce26681622c844ff9891f8017b789ac4 |
| institution | Directory Open Access Journal |
| issn | 2234-1757 |
| language | English |
| last_indexed | 2024-12-16T06:40:43Z |
| publishDate | 2016-03-01 |
| publisher | The Korean Society of Fisheries and Aquatic Science |
| record_format | Article |
| series | Fisheries and Aquatic Sciences |
| spelling | doaj.art-ce26681622c844ff9891f8017b789ac42022-12-21T22:40:42ZengThe Korean Society of Fisheries and Aquatic ScienceFisheries and Aquatic Sciences2234-17572016-03-011911710.1186/s41240-016-0004-1Determination of the dietary lysine requirement by measuring plasma free lysine concentrations in rainbow trout Oncorhynchus mykiss after dorsal aorta cannulationHyeonho Yun0Gunjun Park1Imho Ok2Kumar Katya3Silas Hung4Sungchul C. Bai5Department of Marine Bio-Materials and Aquaculture/Feeds and Foods Nutrition Research Center, Pukyong National UniversityWoosung Feed Co., LtdAqua Leader, Co., LtdDepartment of Marine Bio-Materials and Aquaculture/Feeds and Foods Nutrition Research Center, Pukyong National UniversityDepartment of Animal Science, University of CaliforniaDepartment of Marine Bio-Materials and Aquaculture/Feeds and Foods Nutrition Research Center, Pukyong National UniversityAbstract This study evaluated the dietary lysine requirement by measuring the plasma free lysine concentrations in rainbow trout, Oncorhynchus mykiss after dorsal aorta cannulation. A basal diet containing 36.6 % crude protein (29.6 % crystalline amino acids mixture, 5 % casein and 2 % gelatin) was formulated to one of the seven L-amino acid based diets containing graded levels of lysine (0.72, 1.12, 1.52, 1.92, 2.32, 2.72 or 3.52 % dry diet). A total of 35 fish averaging 512 ± 6.8 g (mean ± SD) were randomly distributed into seven groups with five fish in each group. After 48 h of feed deprivation, each group of fish was fed one of the experimental diets by intubation at 1 % body weight. Blood samples were taken at 0, 5 and 24 h after intubation. Post-prandial plasma free lysine concentrations (PPlys, 5 h after intubation) of fish fed diets containing ≥ 2.32 % lysine were higher than those of fish fed diets containing ≤ 1.92 % lysine. Post-absorptive free lysine concentrations (PAlys, 24 h after intubation) of fish fed diets containing 2.32 and 3.52 % lysine were higher than those of fish fed diets containing ≤ 1.52 % lysine. The broken-line regression analysis on the basis of PPlys and PAlys indicated that the lysine requirement of rainbow trout could be 2.34 and 2.20 % in diet. Therefore, these results strongly suggested that the dietary lysine requirement based on the broken-line model analyses of PPlys and PAlys could be greater than 2.2 but less than 2.34 % (corresponding to be 6.01 % ≤, but ≤ 6.39 % in dietary protein basis, respectively) in rainbow trout. Also, these results shown that the quantitative estimation of lysine requirement by using PPlys and PAlys could be an acceptable method in fish.http://link.springer.com/article/10.1186/s41240-016-0004-1Oncorhynchus mykissRainbow troutLysinePlasmaDorsal aorta cannulation |
| spellingShingle | Hyeonho Yun Gunjun Park Imho Ok Kumar Katya Silas Hung Sungchul C. Bai Determination of the dietary lysine requirement by measuring plasma free lysine concentrations in rainbow trout Oncorhynchus mykiss after dorsal aorta cannulation Fisheries and Aquatic Sciences Oncorhynchus mykiss Rainbow trout Lysine Plasma Dorsal aorta cannulation |
| title | Determination of the dietary lysine requirement by measuring plasma free lysine concentrations in rainbow trout Oncorhynchus mykiss after dorsal aorta cannulation |
| title_full | Determination of the dietary lysine requirement by measuring plasma free lysine concentrations in rainbow trout Oncorhynchus mykiss after dorsal aorta cannulation |
| title_fullStr | Determination of the dietary lysine requirement by measuring plasma free lysine concentrations in rainbow trout Oncorhynchus mykiss after dorsal aorta cannulation |
| title_full_unstemmed | Determination of the dietary lysine requirement by measuring plasma free lysine concentrations in rainbow trout Oncorhynchus mykiss after dorsal aorta cannulation |
| title_short | Determination of the dietary lysine requirement by measuring plasma free lysine concentrations in rainbow trout Oncorhynchus mykiss after dorsal aorta cannulation |
| title_sort | determination of the dietary lysine requirement by measuring plasma free lysine concentrations in rainbow trout oncorhynchus mykiss after dorsal aorta cannulation |
| topic | Oncorhynchus mykiss Rainbow trout Lysine Plasma Dorsal aorta cannulation |
| url | http://link.springer.com/article/10.1186/s41240-016-0004-1 |
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