Optimization extraction and bioactivities of polysaccharide from wild Russula griseocarnosa
The extraction conditions and biological activities of polysaccharides from wild Russula griseocarnosa (PRG) were investigated. Response Surface Methodology (RSM) with a Box-Behnken Design (BBD) was used to optimize extraction conditions. The optimal extraction parameters of PRG were as follows: ext...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2017-05-01
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| Series: | Saudi Pharmaceutical Journal |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1319016417300828 |
| _version_ | 1818315387676131328 |
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| author | Ye Yuan Yang Liu Mengdi Liu Qian Chen Yuanyuan Jiao Yan Liu Zhaoli Meng |
| author_facet | Ye Yuan Yang Liu Mengdi Liu Qian Chen Yuanyuan Jiao Yan Liu Zhaoli Meng |
| author_sort | Ye Yuan |
| collection | DOAJ |
| description | The extraction conditions and biological activities of polysaccharides from wild Russula griseocarnosa (PRG) were investigated. Response Surface Methodology (RSM) with a Box-Behnken Design (BBD) was used to optimize extraction conditions. The optimal extraction parameters of PRG were as follows: extracting time 4 h, extraction temperature 77.3 °C and liquid-solid ratio 42.5 g/L. Furthermore, the data demonstrated that PRG exhibited antioxidant activities evidenced by reducing power to scavenge the DPPH, ABTS, hydroxyl radical and superoxide radical. PRG showed the activity of anti-cervical carcinoma cells Hela and Siha. In conclusion this study offered an efficient extraction method of wild Russula griseocarnosa polysaccharide, and the results suggested PRG had good antioxidant and inhibitory activities against cervical carcinoma cells, and PRG could be developed as a novel natural functional food. Keywords: Russula griseocarnosa, Response surface methodology, Polysaccharide, Antioxidant activity, Anti-tumor activity |
| first_indexed | 2024-12-13T09:04:44Z |
| format | Article |
| id | doaj.art-ce26f7c37a144011b8834ca37af9413c |
| institution | Directory Open Access Journal |
| issn | 1319-0164 |
| language | English |
| last_indexed | 2024-12-13T09:04:44Z |
| publishDate | 2017-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Saudi Pharmaceutical Journal |
| spelling | doaj.art-ce26f7c37a144011b8834ca37af9413c2022-12-21T23:53:05ZengElsevierSaudi Pharmaceutical Journal1319-01642017-05-01254523530Optimization extraction and bioactivities of polysaccharide from wild Russula griseocarnosaYe Yuan0Yang Liu1Mengdi Liu2Qian Chen3Yuanyuan Jiao4Yan Liu5Zhaoli Meng6The Department of Medicine Laboratory, The First Hospital, Jilin University, Changchun 130061, ChinaThe Engineering Research Centre of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130018, ChinaThe Engineering Research Centre of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130018, ChinaInstitute of Translational Medicine, The First Hospital, Jilin University, Changchun 130061, ChinaInstitute of Translational Medicine, The First Hospital, Jilin University, Changchun 130061, ChinaSchool of Life Sciences, Jilin University, Changchun 130012, China; Corresponding authors.Institute of Translational Medicine, The First Hospital, Jilin University, Changchun 130061, China; Corresponding authors.The extraction conditions and biological activities of polysaccharides from wild Russula griseocarnosa (PRG) were investigated. Response Surface Methodology (RSM) with a Box-Behnken Design (BBD) was used to optimize extraction conditions. The optimal extraction parameters of PRG were as follows: extracting time 4 h, extraction temperature 77.3 °C and liquid-solid ratio 42.5 g/L. Furthermore, the data demonstrated that PRG exhibited antioxidant activities evidenced by reducing power to scavenge the DPPH, ABTS, hydroxyl radical and superoxide radical. PRG showed the activity of anti-cervical carcinoma cells Hela and Siha. In conclusion this study offered an efficient extraction method of wild Russula griseocarnosa polysaccharide, and the results suggested PRG had good antioxidant and inhibitory activities against cervical carcinoma cells, and PRG could be developed as a novel natural functional food. Keywords: Russula griseocarnosa, Response surface methodology, Polysaccharide, Antioxidant activity, Anti-tumor activityhttp://www.sciencedirect.com/science/article/pii/S1319016417300828 |
| spellingShingle | Ye Yuan Yang Liu Mengdi Liu Qian Chen Yuanyuan Jiao Yan Liu Zhaoli Meng Optimization extraction and bioactivities of polysaccharide from wild Russula griseocarnosa Saudi Pharmaceutical Journal |
| title | Optimization extraction and bioactivities of polysaccharide from wild Russula griseocarnosa |
| title_full | Optimization extraction and bioactivities of polysaccharide from wild Russula griseocarnosa |
| title_fullStr | Optimization extraction and bioactivities of polysaccharide from wild Russula griseocarnosa |
| title_full_unstemmed | Optimization extraction and bioactivities of polysaccharide from wild Russula griseocarnosa |
| title_short | Optimization extraction and bioactivities of polysaccharide from wild Russula griseocarnosa |
| title_sort | optimization extraction and bioactivities of polysaccharide from wild russula griseocarnosa |
| url | http://www.sciencedirect.com/science/article/pii/S1319016417300828 |
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